File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Immunomodulatory compounds from Pestalotiopsis leucothës, an endophytic fungus from Tripterygium wilfordii

TitleImmunomodulatory compounds from Pestalotiopsis leucothës, an endophytic fungus from Tripterygium wilfordii
Authors
KeywordsCytokines
Cytotoxicity
Fungal compounds
Immune mediated diseases
Immunomodulation
Immunomodulatory drugs
Medicinal fungal compounds
Pestalotiopsis leucothës
Traditional Chinese medicine
Tripterygium wilfordii
Issue Date2005
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie
Citation
Life Sciences, 2005, v. 78 n. 2, p. 147-156 How to Cite?
AbstractThe immunomodulatory effects of three compounds designated BS, GS, and YS produced by Pestalotiopsis leucothës, an endophytic fungus isolated from Tripterygium wilfordii, were evaluated. The 50% inhibition concentration (IC50) value of BS in the proliferative assay with various stimulating agents such as phytohemagglutinin-M (PHA-M), phorbol myristate acetate (PMA)/ionomycin, mixed lymphocyte reaction (MLR) and poke weed mitogen (PWM) was 0.35, 1.6, 0.8 and 5.4 μg/ml, respectively. In addition, BS significantly inhibited the production of cytokines such as interleukin (IL)-1β, IL-2, interferon (IFN)-γ and tumor necrosis factor (TNF)-α, by peripheral blood mononuclear cells (PBMNC) and soluble IL-2 receptor expression at concentrations greater than 1 μg/ml. Inhibition of PHA stimulated PBMNC proliferation and IL-2 and sIL-2R production by BS indicates that it is a T-cell specific immunosuppressant. However, BS also moderately inhibited immunoglobulin (Ig) G and M at concentrations greater than 1 μg/ml suggesting that it also has B cell immunosuppressive effects. YS was 10% less active than BS in all assay systems. In contrast, GS exhibited both suppression and enhancement of PBMNC proliferation in the presence of various stimulants. However, GS inhibited PWM stimulated PBMNC proliferation and IL-4 and IgG and IgM production at concentrations above 1 μg/ml. All three fungal compounds altered the percentage of T-lymphocyte subpopulations only at high concentrations. Cell viability was not affected at the immunosuppressive concentrations of these compounds. In conclusion, work from our laboratory has identified three potentially potent immunomodulatory compounds from P. leucothës. These compounds have variable effects on T- and B-cells and monocytes. They may partially explain the immunosuppressive activity of T. wilfordii. In addition, they may represent a new source of immunomodulatory compounds for the treatment of human immune mediated diseases. © 2005 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/162895
ISSN
2015 Impact Factor: 2.685
2015 SCImago Journal Rankings: 1.056
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKumar, DSSen_HK
dc.contributor.authorLau, CSen_HK
dc.contributor.authorWan, JMFen_HK
dc.contributor.authorYang, Den_HK
dc.contributor.authorHyde, KDen_HK
dc.date.accessioned2012-09-05T05:24:53Z-
dc.date.available2012-09-05T05:24:53Z-
dc.date.issued2005en_HK
dc.identifier.citationLife Sciences, 2005, v. 78 n. 2, p. 147-156en_HK
dc.identifier.issn0024-3205en_HK
dc.identifier.urihttp://hdl.handle.net/10722/162895-
dc.description.abstractThe immunomodulatory effects of three compounds designated BS, GS, and YS produced by Pestalotiopsis leucothës, an endophytic fungus isolated from Tripterygium wilfordii, were evaluated. The 50% inhibition concentration (IC50) value of BS in the proliferative assay with various stimulating agents such as phytohemagglutinin-M (PHA-M), phorbol myristate acetate (PMA)/ionomycin, mixed lymphocyte reaction (MLR) and poke weed mitogen (PWM) was 0.35, 1.6, 0.8 and 5.4 μg/ml, respectively. In addition, BS significantly inhibited the production of cytokines such as interleukin (IL)-1β, IL-2, interferon (IFN)-γ and tumor necrosis factor (TNF)-α, by peripheral blood mononuclear cells (PBMNC) and soluble IL-2 receptor expression at concentrations greater than 1 μg/ml. Inhibition of PHA stimulated PBMNC proliferation and IL-2 and sIL-2R production by BS indicates that it is a T-cell specific immunosuppressant. However, BS also moderately inhibited immunoglobulin (Ig) G and M at concentrations greater than 1 μg/ml suggesting that it also has B cell immunosuppressive effects. YS was 10% less active than BS in all assay systems. In contrast, GS exhibited both suppression and enhancement of PBMNC proliferation in the presence of various stimulants. However, GS inhibited PWM stimulated PBMNC proliferation and IL-4 and IgG and IgM production at concentrations above 1 μg/ml. All three fungal compounds altered the percentage of T-lymphocyte subpopulations only at high concentrations. Cell viability was not affected at the immunosuppressive concentrations of these compounds. In conclusion, work from our laboratory has identified three potentially potent immunomodulatory compounds from P. leucothës. These compounds have variable effects on T- and B-cells and monocytes. They may partially explain the immunosuppressive activity of T. wilfordii. In addition, they may represent a new source of immunomodulatory compounds for the treatment of human immune mediated diseases. © 2005 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescieen_HK
dc.relation.ispartofLife Sciencesen_HK
dc.rightsLife Sciences. Copyright © Elsevier Inc.-
dc.subjectCytokinesen_HK
dc.subjectCytotoxicityen_HK
dc.subjectFungal compoundsen_HK
dc.subjectImmune mediated diseasesen_HK
dc.subjectImmunomodulationen_HK
dc.subjectImmunomodulatory drugsen_HK
dc.subjectMedicinal fungal compoundsen_HK
dc.subjectPestalotiopsis leucothësen_HK
dc.subjectTraditional Chinese medicineen_HK
dc.subjectTripterygium wilfordiien_HK
dc.subject.meshAscomycota - Chemistryen_US
dc.subject.meshCell Proliferation - Drug Effectsen_US
dc.subject.meshCell Survival - Drug Effectsen_US
dc.subject.meshCytokines - Biosynthesisen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshFlow Cytometryen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoglobulin G - Biosynthesisen_US
dc.subject.meshImmunoglobulin M - Biosynthesisen_US
dc.subject.meshImmunologic Factors - Chemistry - Pharmacologyen_US
dc.subject.meshLymphocyte Culture Test, Mixeden_US
dc.subject.meshLymphocytes - Drug Effectsen_US
dc.subject.meshMitogens - Metabolismen_US
dc.subject.meshMonocytes - Drug Effects - Metabolismen_US
dc.subject.meshPhenotypeen_US
dc.subject.meshReceptors, Interleukin-2 - Biosynthesisen_US
dc.subject.meshTh1 Cells - Drug Effects - Metabolismen_US
dc.subject.meshTh2 Cells - Drug Effects - Metabolismen_US
dc.subject.meshTripterygium - Microbiologyen_US
dc.titleImmunomodulatory compounds from Pestalotiopsis leucothës, an endophytic fungus from Tripterygium wilfordiien_HK
dc.typeArticleen_HK
dc.identifier.emailLau, CS: cslau@hku.hken_HK
dc.identifier.emailWan, JMF: jmfwan@hku.hken_HK
dc.identifier.emailYang, D: yangdan@hku.hken_HK
dc.identifier.authorityLau, CS=rp01348en_HK
dc.identifier.authorityWan, JMF=rp00798en_HK
dc.identifier.authorityYang, D=rp00825en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.lfs.2005.04.050en_HK
dc.identifier.pmid16107268-
dc.identifier.scopuseid_2-s2.0-27644447426en_HK
dc.identifier.hkuros115119-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-27644447426&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume78en_HK
dc.identifier.issue2en_HK
dc.identifier.spage147en_HK
dc.identifier.epage156en_HK
dc.identifier.isiWOS:000233187400005-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridKumar, DSS=7402293889en_HK
dc.identifier.scopusauthoridLau, CS=14035682100en_HK
dc.identifier.scopusauthoridWan, JMF=8930305000en_HK
dc.identifier.scopusauthoridYang, D=7404800756en_HK
dc.identifier.scopusauthoridHyde, KD=7102588111en_HK
dc.identifier.citeulike9255471-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats