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Article: Coronaviral hypothetical and structural proteins were found in the intestinal surface enterocytes and pneumocytes of severe acute respiratory syndrome (SARS)

TitleCoronaviral hypothetical and structural proteins were found in the intestinal surface enterocytes and pneumocytes of severe acute respiratory syndrome (SARS)
Authors
Issue Date2005
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/modpathol/
Citation
Modern Pathology, 2005, v. 18 n. 11, p. 1432-1439 How to Cite?
AbstractSevere acute respiratory syndrome (SARS) is a newly emerging infectious disease that haunted the world from November 2002 to July 2003. Little is known about the biology and pathophysiology of the novel coronavirus that causes SARS. The tissue and cellular distributions of coronaviral hypothetical and structural proteins in SARS were investigated. Antibodies against the hypothetical (SARS 3a, 3b, 6, 7a and 9b) and structural proteins (envelope, membrane, nucleocapsid and spike) of the coronavirus were generated from predicted antigenic epitopes of each protein. The presence of these proteins were first verified in coronavirus-infected Vero E6 tissue culture model. Immunohistochemical studies on different human tissues, including a cohort of nine autopsies, two liver biopsies and intestinal biopsies of SARS patients, further confirmed the existence of coronaviral hypothetical and structural proteins in the cytoplasm of pneumocytes and small intestinal surface enterocytes in SARS patients. With this vast array of antibodies, no signal was observed in other cell types including those organs in which reverse transcriptase-polymerase chain reactions were reported to be positive. Structural proteins and the functionally undefined hypothetical proteins were expressed in coronavirus-infected cells with distinct expression pattern in different organs in SARS patients. These antipeptide antibodies can be useful for the diagnosis of SARS at the tissue level. © 2005 USCAP, Inc All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/162891
ISSN
2015 Impact Factor: 5.485
2015 SCImago Journal Rankings: 2.803
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, WSen_US
dc.contributor.authorWu, Cen_US
dc.contributor.authorChow, SCSen_US
dc.contributor.authorCheung, Ten_US
dc.contributor.authorTo, KFen_US
dc.contributor.authorLeung, WKen_US
dc.contributor.authorChan, PKSen_US
dc.contributor.authorLee, KCen_US
dc.contributor.authorNg, HKen_US
dc.contributor.authorAu, DMYen_US
dc.contributor.authorLo, AWIen_US
dc.date.accessioned2012-09-05T05:24:52Z-
dc.date.available2012-09-05T05:24:52Z-
dc.date.issued2005en_US
dc.identifier.citationModern Pathology, 2005, v. 18 n. 11, p. 1432-1439en_US
dc.identifier.issn0893-3952en_US
dc.identifier.urihttp://hdl.handle.net/10722/162891-
dc.description.abstractSevere acute respiratory syndrome (SARS) is a newly emerging infectious disease that haunted the world from November 2002 to July 2003. Little is known about the biology and pathophysiology of the novel coronavirus that causes SARS. The tissue and cellular distributions of coronaviral hypothetical and structural proteins in SARS were investigated. Antibodies against the hypothetical (SARS 3a, 3b, 6, 7a and 9b) and structural proteins (envelope, membrane, nucleocapsid and spike) of the coronavirus were generated from predicted antigenic epitopes of each protein. The presence of these proteins were first verified in coronavirus-infected Vero E6 tissue culture model. Immunohistochemical studies on different human tissues, including a cohort of nine autopsies, two liver biopsies and intestinal biopsies of SARS patients, further confirmed the existence of coronaviral hypothetical and structural proteins in the cytoplasm of pneumocytes and small intestinal surface enterocytes in SARS patients. With this vast array of antibodies, no signal was observed in other cell types including those organs in which reverse transcriptase-polymerase chain reactions were reported to be positive. Structural proteins and the functionally undefined hypothetical proteins were expressed in coronavirus-infected cells with distinct expression pattern in different organs in SARS patients. These antipeptide antibodies can be useful for the diagnosis of SARS at the tissue level. © 2005 USCAP, Inc All rights reserved.en_US
dc.languageengen_US
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/modpathol/en_US
dc.relation.ispartofModern Pathologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Viral - Diagnostic Useen_US
dc.subject.meshEnterocytes - Virologyen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshIn Situ Hybridization, Fluorescenceen_US
dc.subject.meshLung - Virologyen_US
dc.subject.meshSars Virus - Immunologyen_US
dc.subject.meshSevere Acute Respiratory Syndrome - Virologyen_US
dc.subject.meshViral Structural Proteins - Immunology - Metabolismen_US
dc.titleCoronaviral hypothetical and structural proteins were found in the intestinal surface enterocytes and pneumocytes of severe acute respiratory syndrome (SARS)en_US
dc.typeArticleen_US
dc.identifier.emailLeung, WK:waikleung@hku.hken_US
dc.identifier.authorityLeung, WK=rp01479en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1038/modpathol.3800439en_US
dc.identifier.pmid15920543-
dc.identifier.scopuseid_2-s2.0-27144482376en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-27144482376&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume18en_US
dc.identifier.issue11en_US
dc.identifier.spage1432en_US
dc.identifier.epage1439en_US
dc.identifier.isiWOS:000232807500005-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridChan, WS=37041929300en_US
dc.identifier.scopusauthoridWu, C=7501670669en_US
dc.identifier.scopusauthoridChow, SCS=8929262700en_US
dc.identifier.scopusauthoridCheung, T=8929262800en_US
dc.identifier.scopusauthoridTo, KF=7101911940en_US
dc.identifier.scopusauthoridLeung, WK=7201504523en_US
dc.identifier.scopusauthoridChan, PKS=7403497792en_US
dc.identifier.scopusauthoridLee, KC=7501503975en_US
dc.identifier.scopusauthoridNg, HK=7401619354en_US
dc.identifier.scopusauthoridAu, DMY=36796849400en_US
dc.identifier.scopusauthoridLo, AWI=7102780722en_US
dc.identifier.citeulike199224-

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