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Article: Anti-proliferative and pro-apoptotic effects of herbal medicine on hepatic stellate cell
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TitleAnti-proliferative and pro-apoptotic effects of herbal medicine on hepatic stellate cell
 
AuthorsChor, SY1
Hui, AY1
To, KF2
Chan, KK1
Go, YY1
Chan, HLY1
Leung, WK1
Sung, JJY1
 
Issue Date2005
 
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharm
 
CitationJournal Of Ethnopharmacology, 2005, v. 100 n. 1-2, p. 180-186 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jep.2005.02.036
 
AbstractHepatic stellate cells (HSC) play a central role in hepatic fibrosis and compounds that promote apoptosis in HSC may have anti-fibrotic potentials. Herbal medicine has long been used in chronic liver disease but there is little scientific evidence for their actions. The present study investigated the effects of 14 commonly used herbs on cellular proliferation and apoptosis of a rat hepatic stellate cell line, HSC-T6 and the underlying mechanism of herb-induced apoptosis. HSC-T6 cell were incubated with herbal extracts and their proliferation was assessed by colorimetric assay. Apoptosis was measured and confirmed by flow cytometry, terminal transferase uridyl nick end labeling (TUNEL) assay and morphological features in hematoxylin and eosin staining. Apoptotic pathways involving Fas receptor and Bcl-2 family were investigated by Western blot. Five herbs, namely Angelica sinensis (AS), Carthamus tinctorius (CT), Ligusticum chuanxiong (LC), Salvia miltiorrhiza (SM) and Stephania tetrandra (ST) demonstrated both anti-proliferative and pro-apoptotic activities in HSC-T6. The highest potency was detected in SM and ST with 51.63 and 44.52% of HSC-T6 showing apoptotic changes, respectively. This was associated with upregulation of Fas and Bax and down-regulation of Bcl-xL in HSC. Fas ligand and Bcl2 expressions remained unchanged. The potential anti-fibrotic effect of herbal medicine warrants further evaluation. © 2005 Elsevier Ireland Ltd. All rights reserved.
 
ISSN0378-8741
2012 Impact Factor: 2.755
2012 SCImago Journal Rankings: 0.994
 
DOIhttp://dx.doi.org/10.1016/j.jep.2005.02.036
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorChor, SY
 
dc.contributor.authorHui, AY
 
dc.contributor.authorTo, KF
 
dc.contributor.authorChan, KK
 
dc.contributor.authorGo, YY
 
dc.contributor.authorChan, HLY
 
dc.contributor.authorLeung, WK
 
dc.contributor.authorSung, JJY
 
dc.date.accessioned2012-09-05T05:24:35Z
 
dc.date.available2012-09-05T05:24:35Z
 
dc.date.issued2005
 
dc.description.abstractHepatic stellate cells (HSC) play a central role in hepatic fibrosis and compounds that promote apoptosis in HSC may have anti-fibrotic potentials. Herbal medicine has long been used in chronic liver disease but there is little scientific evidence for their actions. The present study investigated the effects of 14 commonly used herbs on cellular proliferation and apoptosis of a rat hepatic stellate cell line, HSC-T6 and the underlying mechanism of herb-induced apoptosis. HSC-T6 cell were incubated with herbal extracts and their proliferation was assessed by colorimetric assay. Apoptosis was measured and confirmed by flow cytometry, terminal transferase uridyl nick end labeling (TUNEL) assay and morphological features in hematoxylin and eosin staining. Apoptotic pathways involving Fas receptor and Bcl-2 family were investigated by Western blot. Five herbs, namely Angelica sinensis (AS), Carthamus tinctorius (CT), Ligusticum chuanxiong (LC), Salvia miltiorrhiza (SM) and Stephania tetrandra (ST) demonstrated both anti-proliferative and pro-apoptotic activities in HSC-T6. The highest potency was detected in SM and ST with 51.63 and 44.52% of HSC-T6 showing apoptotic changes, respectively. This was associated with upregulation of Fas and Bax and down-regulation of Bcl-xL in HSC. Fas ligand and Bcl2 expressions remained unchanged. The potential anti-fibrotic effect of herbal medicine warrants further evaluation. © 2005 Elsevier Ireland Ltd. All rights reserved.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationJournal Of Ethnopharmacology, 2005, v. 100 n. 1-2, p. 180-186 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jep.2005.02.036
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.jep.2005.02.036
 
dc.identifier.epage186
 
dc.identifier.issn0378-8741
2012 Impact Factor: 2.755
2012 SCImago Journal Rankings: 0.994
 
dc.identifier.issue1-2
 
dc.identifier.pmid15950418
 
dc.identifier.scopuseid_2-s2.0-23044482864
 
dc.identifier.spage180
 
dc.identifier.urihttp://hdl.handle.net/10722/162868
 
dc.identifier.volume100
 
dc.languageeng
 
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharm
 
dc.publisher.placeIreland
 
dc.relation.ispartofJournal of Ethnopharmacology
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAnimals
 
dc.subject.meshApoptosis - Drug Effects
 
dc.subject.meshCell Line
 
dc.subject.meshCell Proliferation - Drug Effects
 
dc.subject.meshDna Fragmentation
 
dc.subject.meshDose-Response Relationship, Drug
 
dc.subject.meshDrugs, Chinese Herbal - Pharmacology
 
dc.subject.meshHepatocytes - Drug Effects
 
dc.subject.meshRats
 
dc.titleAnti-proliferative and pro-apoptotic effects of herbal medicine on hepatic stellate cell
 
dc.typeArticle
 
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Author Affiliations
  1. Prince of Wales Hospital Hong Kong
  2. Chinese University of Hong Kong