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Article: Quantitative detection of methylated SOCS-1 , a tumor suppressor gene, by a modified protocol of quantitative real time methylation-specific PCR using SYBR green and its use in early gastric cancer detection.

TitleQuantitative detection of methylated SOCS-1 , a tumor suppressor gene, by a modified protocol of quantitative real time methylation-specific PCR using SYBR green and its use in early gastric cancer detection.
Authors
Issue Date2004
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0141-5492
Citation
Biotechnology Letters, 2004, v. 26 n. 16, p. 1289-1293 How to Cite?
AbstractAlthough methylation-specific PCR (MSP) is a sensitive technique in the detection of DNA hypermethylation, it is not quantitative. Here we described a modified PCR protocol to quantify methylated SOCS-1 gene by real time MSP using SYBR green, which involves an additional PCR step after the 72 degrees C extension step. This modified protocol is also useful in the quantitative detection of methylated SOCS-1 gene in serum samples of gastric cancer patients.
Persistent Identifierhttp://hdl.handle.net/10722/162819
ISSN
2015 Impact Factor: 1.639
2015 SCImago Journal Rankings: 0.591
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChan, MWen_US
dc.contributor.authorChu, ESen_US
dc.contributor.authorTo, KFen_US
dc.contributor.authorLeung, WKen_US
dc.date.accessioned2012-09-05T05:23:56Z-
dc.date.available2012-09-05T05:23:56Z-
dc.date.issued2004en_US
dc.identifier.citationBiotechnology Letters, 2004, v. 26 n. 16, p. 1289-1293en_US
dc.identifier.issn0141-5492en_US
dc.identifier.urihttp://hdl.handle.net/10722/162819-
dc.description.abstractAlthough methylation-specific PCR (MSP) is a sensitive technique in the detection of DNA hypermethylation, it is not quantitative. Here we described a modified PCR protocol to quantify methylated SOCS-1 gene by real time MSP using SYBR green, which involves an additional PCR step after the 72 degrees C extension step. This modified protocol is also useful in the quantitative detection of methylated SOCS-1 gene in serum samples of gastric cancer patients.en_US
dc.languageengen_US
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0141-5492en_US
dc.relation.ispartofBiotechnology lettersen_US
dc.subject.meshAdulten_US
dc.subject.meshDna Methylationen_US
dc.subject.meshFemaleen_US
dc.subject.meshGenes, Tumor Suppressoren_US
dc.subject.meshGenetic Predisposition To Disease - Geneticsen_US
dc.subject.meshGenetic Testing - Methodsen_US
dc.subject.meshHumansen_US
dc.subject.meshIntracellular Signaling Peptides And Proteins - Blood - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshMethylationen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshOrganic Chemicalsen_US
dc.subject.meshPolymerase Chain Reaction - Methodsen_US
dc.subject.meshRepressor Proteins - Blood - Metabolismen_US
dc.subject.meshReproducibility Of Resultsen_US
dc.subject.meshSensitivity And Specificityen_US
dc.subject.meshStomach Neoplasms - Blood - Genetics - Metabolismen_US
dc.subject.meshSuppressor Of Cytokine Signaling Proteinsen_US
dc.subject.meshTumor Markers, Biological - Blooden_US
dc.titleQuantitative detection of methylated SOCS-1 , a tumor suppressor gene, by a modified protocol of quantitative real time methylation-specific PCR using SYBR green and its use in early gastric cancer detection.en_US
dc.typeArticleen_US
dc.identifier.emailLeung, WK:waikleung@hku.hken_US
dc.identifier.authorityLeung, WK=rp01479en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1023/B:BILE.0000044922.43572.2d-
dc.identifier.pmid15483389-
dc.identifier.scopuseid_2-s2.0-16544367808en_US
dc.identifier.volume26en_US
dc.identifier.issue16en_US
dc.identifier.spage1289en_US
dc.identifier.epage1293en_US
dc.identifier.isiWOS:000224962100008-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridChan, MW=7402597788en_US
dc.identifier.scopusauthoridChu, ES=8631130300en_US
dc.identifier.scopusauthoridTo, KF=7101911940en_US
dc.identifier.scopusauthoridLeung, WK=7201504523en_US

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