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Article: Intestinal trefoil factor promotes invasion in non-tumorigenic Rat-2 fibroblast cell

TitleIntestinal trefoil factor promotes invasion in non-tumorigenic Rat-2 fibroblast cell
Authors
Issue Date2005
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/regpep
Citation
Regulatory Peptides, 2005, v. 127 n. 1-3, p. 87-94 How to Cite?
AbstractIntestinal trefoil factor (TFF3) is essential in regulating cell migration and maintaining mucosal integrity in gastrointestinal tract. We previously showed that TFF3 was overexpressed in gastric carcinoma. Whether TFF3 possesses malignant potential is not fully elucidated. We sought to investigate the effects of inducting TFF3 expression in a non-malignant rat fibroblast cell line (Rat-2) on the cell proliferation, invasion and the genes regulating cell invasion. Invasiveness and proliferation of transfected Rat-2 cell line were assessed using in vitro invasion chamber assay and colorimetric MTS assay. Differential mRNA expressions of invasion-related genes, namely, metalloproteinases (MMP-9), tissue inhibitors of metalloproteinases (TIMP-1), β-catenin and E-cadherin, were determined by quantitative real-time polymerase chain reaction (PCR). We showed that TFF3 did not inhibit the proliferation of Rat-2 cells. We also demonstrated that transfection of TFF3 significantly promoted invasion of Rat-2 cells by 1.4- to 2.2-folds. There was an upregulation of β-catenin (13.1-23.0%) and MMP-9 (43.4-92.2%) mRNA expression levels, and downregulation of E-cadherin (25.6-33.8%) and TIMP-1 (31.5-37.8%) in TFF3-transfected cells compared to controls during 48-h incubation. Our results suggested that TFF3 possesses malignant potential through promotion of cell invasiveness and alteration of invasion-related genes. © 2004 Published by Elsevier B.V.
Persistent Identifierhttp://hdl.handle.net/10722/162783
ISSN
2015 Impact Factor: 1.813
2015 SCImago Journal Rankings: 0.915
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, VYWen_US
dc.contributor.authorChan, MWYen_US
dc.contributor.authorLeung, WKen_US
dc.contributor.authorLeung, PSen_US
dc.contributor.authorSung, JJYen_US
dc.contributor.authorChan, FKLen_US
dc.date.accessioned2012-09-05T05:23:28Z-
dc.date.available2012-09-05T05:23:28Z-
dc.date.issued2005en_US
dc.identifier.citationRegulatory Peptides, 2005, v. 127 n. 1-3, p. 87-94en_US
dc.identifier.issn0167-0115en_US
dc.identifier.urihttp://hdl.handle.net/10722/162783-
dc.description.abstractIntestinal trefoil factor (TFF3) is essential in regulating cell migration and maintaining mucosal integrity in gastrointestinal tract. We previously showed that TFF3 was overexpressed in gastric carcinoma. Whether TFF3 possesses malignant potential is not fully elucidated. We sought to investigate the effects of inducting TFF3 expression in a non-malignant rat fibroblast cell line (Rat-2) on the cell proliferation, invasion and the genes regulating cell invasion. Invasiveness and proliferation of transfected Rat-2 cell line were assessed using in vitro invasion chamber assay and colorimetric MTS assay. Differential mRNA expressions of invasion-related genes, namely, metalloproteinases (MMP-9), tissue inhibitors of metalloproteinases (TIMP-1), β-catenin and E-cadherin, were determined by quantitative real-time polymerase chain reaction (PCR). We showed that TFF3 did not inhibit the proliferation of Rat-2 cells. We also demonstrated that transfection of TFF3 significantly promoted invasion of Rat-2 cells by 1.4- to 2.2-folds. There was an upregulation of β-catenin (13.1-23.0%) and MMP-9 (43.4-92.2%) mRNA expression levels, and downregulation of E-cadherin (25.6-33.8%) and TIMP-1 (31.5-37.8%) in TFF3-transfected cells compared to controls during 48-h incubation. Our results suggested that TFF3 possesses malignant potential through promotion of cell invasiveness and alteration of invasion-related genes. © 2004 Published by Elsevier B.V.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/regpepen_US
dc.relation.ispartofRegulatory Peptidesen_US
dc.subject.meshActins - Genetics - Metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCadherins - Genetics - Metabolismen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCell Proliferationen_US
dc.subject.meshCytoskeletal Proteins - Genetics - Metabolismen_US
dc.subject.meshFibroblasts - Cytology - Metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshMatrix Metalloproteinase 9 - Genetics - Metabolismen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshNeoplasm Invasivenessen_US
dc.subject.meshNeuropeptides - Genetics - Metabolismen_US
dc.subject.meshRna, Messenger - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshTissue Inhibitor Of Metalloproteinase-1 - Genetics - Metabolismen_US
dc.subject.meshTrans-Activators - Genetics - Metabolismen_US
dc.subject.meshBeta Cateninen_US
dc.titleIntestinal trefoil factor promotes invasion in non-tumorigenic Rat-2 fibroblast cellen_US
dc.typeArticleen_US
dc.identifier.emailLeung, WK:waikleung@hku.hken_US
dc.identifier.authorityLeung, WK=rp01479en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.regpep.2004.10.016en_US
dc.identifier.pmid15680474-
dc.identifier.scopuseid_2-s2.0-13144257729en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-13144257729&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume127en_US
dc.identifier.issue1-3en_US
dc.identifier.spage87en_US
dc.identifier.epage94en_US
dc.identifier.isiWOS:000227023900011-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridChan, VYW=8599737600en_US
dc.identifier.scopusauthoridChan, MWY=7402597788en_US
dc.identifier.scopusauthoridLeung, WK=7201504523en_US
dc.identifier.scopusauthoridLeung, PS=7401748938en_US
dc.identifier.scopusauthoridSung, JJY=24473715000en_US
dc.identifier.scopusauthoridChan, FKL=7202586434en_US

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