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Article: Immunization with Attenuated Salmonella typhimurium Producing Catalase in Protection against Gastric Helicobacter pylori Infection in Mice

TitleImmunization with Attenuated Salmonella typhimurium Producing Catalase in Protection against Gastric Helicobacter pylori Infection in Mice
Authors
Issue Date2003
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/HEL
Citation
Helicobacter, 2003, v. 8 n. 6, p. 613-625 How to Cite?
AbstractAim. To evaluate the protective effect of live attenuated Salmonella typhimurium expressing catalase against gastric Helicobacter pylori infection in mice, and to explore the underlying mechanisms of the protective immune reaction. Materials and Methods The H. pylori catalase gene was introduced into attenuated S. typhimurium strain SL3261. C57BL/6 mice were orally immunized with the SL3261 vaccine strain expressing catalase or with SL3261 alone or phosphate-buffered saline (PBS). Mice were sacrificed 4 weeks after immunization and 5 weeks after H. pylori challenge, respectively. Results. All PBS control mice were infected. Eight of 13 (61.5%) mice immunized with the SL3261 vaccine strain and three of 14 (21%) mice immunized with SL3261 alone showed protection against H. pylori infection. Serum anti-H. pylori IgG2a levels of S. typhimurium-immunized mice were higher than those of PBS controls, both before and after H. pylori challenge, while there were no differences for IgG1 and IgA. Similarly, mRNA expression of interleukin (IL)-2, IL-12 and interferon-γin the gastric mucosa of S. typhimurium-immunized mice was significantly higher than that of PBS controls both before and after challenge. Moreover, S. typhimurium-immunized mice were characterized by marked infiltration of lymphocyte and mononuclear cells in the gastric mucosa after challenge. IL-4 and IL-10 were not detected in any of the three groups. IL-6 expression was increased in the PBS group compared with the S. typhimurium-immunized groups after challenge. Conclusions. This study demonstrates that oral immunization of mice with catalase delivered by an attenuated S. typhimurium strain offers protection against H. pylori infection. This protective immunity was mediated through a predominantly Thl-type response and was associated with post-immunization gastritis.
Persistent Identifierhttp://hdl.handle.net/10722/162743
ISSN
2015 Impact Factor: 3.92
2015 SCImago Journal Rankings: 1.131
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, Men_US
dc.contributor.authorChen, Jen_US
dc.contributor.authorLiao, Wen_US
dc.contributor.authorZhu, Sen_US
dc.contributor.authorYu, Jen_US
dc.contributor.authorLeung, WKen_US
dc.contributor.authorHu, Pen_US
dc.contributor.authorSung, JJYen_US
dc.date.accessioned2012-09-05T05:23:02Z-
dc.date.available2012-09-05T05:23:02Z-
dc.date.issued2003en_US
dc.identifier.citationHelicobacter, 2003, v. 8 n. 6, p. 613-625en_US
dc.identifier.issn1083-4389en_US
dc.identifier.urihttp://hdl.handle.net/10722/162743-
dc.description.abstractAim. To evaluate the protective effect of live attenuated Salmonella typhimurium expressing catalase against gastric Helicobacter pylori infection in mice, and to explore the underlying mechanisms of the protective immune reaction. Materials and Methods The H. pylori catalase gene was introduced into attenuated S. typhimurium strain SL3261. C57BL/6 mice were orally immunized with the SL3261 vaccine strain expressing catalase or with SL3261 alone or phosphate-buffered saline (PBS). Mice were sacrificed 4 weeks after immunization and 5 weeks after H. pylori challenge, respectively. Results. All PBS control mice were infected. Eight of 13 (61.5%) mice immunized with the SL3261 vaccine strain and three of 14 (21%) mice immunized with SL3261 alone showed protection against H. pylori infection. Serum anti-H. pylori IgG2a levels of S. typhimurium-immunized mice were higher than those of PBS controls, both before and after H. pylori challenge, while there were no differences for IgG1 and IgA. Similarly, mRNA expression of interleukin (IL)-2, IL-12 and interferon-γin the gastric mucosa of S. typhimurium-immunized mice was significantly higher than that of PBS controls both before and after challenge. Moreover, S. typhimurium-immunized mice were characterized by marked infiltration of lymphocyte and mononuclear cells in the gastric mucosa after challenge. IL-4 and IL-10 were not detected in any of the three groups. IL-6 expression was increased in the PBS group compared with the S. typhimurium-immunized groups after challenge. Conclusions. This study demonstrates that oral immunization of mice with catalase delivered by an attenuated S. typhimurium strain offers protection against H. pylori infection. This protective immunity was mediated through a predominantly Thl-type response and was associated with post-immunization gastritis.en_US
dc.languageengen_US
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/HELen_US
dc.relation.ispartofHelicobacteren_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Bacterial - Blooden_US
dc.subject.meshAntigens, Bacterial - Immunologyen_US
dc.subject.meshBacterial Vaccinesen_US
dc.subject.meshCatalase - Metabolismen_US
dc.subject.meshCytokines - Geneticsen_US
dc.subject.meshFemaleen_US
dc.subject.meshGastric Mucosa - Microbiology - Physiologyen_US
dc.subject.meshGastritis - Immunology - Microbiology - Prevention & Controlen_US
dc.subject.meshHelicobacter Infections - Immunology - Prevention & Controlen_US
dc.subject.meshHelicobacter Pylori - Immunologyen_US
dc.subject.meshImmunization - Methodsen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred C57blen_US
dc.subject.meshPlasmidsen_US
dc.subject.meshRna, Messenger - Analysisen_US
dc.subject.meshSalmonella Typhimurium - Enzymology - Genetics - Immunologyen_US
dc.subject.meshSonicationen_US
dc.titleImmunization with Attenuated Salmonella typhimurium Producing Catalase in Protection against Gastric Helicobacter pylori Infection in Miceen_US
dc.typeArticleen_US
dc.identifier.emailLeung, WK:waikleung@hku.hken_US
dc.identifier.authorityLeung, WK=rp01479en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1523-5378.2003.00182.xen_US
dc.identifier.pmid14632677-
dc.identifier.scopuseid_2-s2.0-0346093636en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0346093636&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume8en_US
dc.identifier.issue6en_US
dc.identifier.spage613en_US
dc.identifier.epage625en_US
dc.identifier.isiWOS:000186798400008-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridChen, M=8642044500en_US
dc.identifier.scopusauthoridChen, J=8347136200en_US
dc.identifier.scopusauthoridLiao, W=12773880500en_US
dc.identifier.scopusauthoridZhu, S=7404391208en_US
dc.identifier.scopusauthoridYu, J=35351306800en_US
dc.identifier.scopusauthoridLeung, WK=7201504523en_US
dc.identifier.scopusauthoridHu, P=7201989582en_US
dc.identifier.scopusauthoridSung, JJY=35405352400en_US

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