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Article: A human TERT C-terminal polypeptide sensitizes HeLa cells to H2O2-induced senescence without affecting telomerase enzymatic activity

TitleA human TERT C-terminal polypeptide sensitizes HeLa cells to H2O2-induced senescence without affecting telomerase enzymatic activity
Authors
KeywordsHeLa cells
hTERT C-terminus
Oxidative stress
Senescence
Issue Date2003
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 2003, v. 301 n. 3, p. 627-632 How to Cite?
AbstractWe have constructed a 27-kDa hTERT C-terminal polypeptide (hTERTC27) devoid of domains required for telomerase activity and demonstrated that it is capable of nuclear translocation/telomere-end targeting. Here we showed that expression of a low level of hTERTC27 renders hTERT positive HeLa cells sensitive to H2O2-induced oxidative stress and subsequent cell senescence. The senescence-associated gene, the cyclin/cdk inhibitor p21 Waf1, was up-regulated. This occurs without changing the expression of endogenous hTERT, causing significant telomere shortening or inhibiting telomerase activity. Results from this study suggest for the first time that in addition to telomerase activity, the C-terminus of hTERT also plays a role in hTERT-mediated cellular resistance to oxidative stress. © 2003 Elsevier Science (USA). All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/162684
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHuang, JJen_HK
dc.contributor.authorBai, YXen_HK
dc.contributor.authorHan, SWen_HK
dc.contributor.authorNg, SSMen_HK
dc.contributor.authorJing, DDen_HK
dc.contributor.authorWong, BCYen_HK
dc.contributor.authorHuang, CFen_HK
dc.contributor.authorKung, HFen_HK
dc.contributor.authorLin, MCMen_HK
dc.date.accessioned2012-09-05T05:22:19Z-
dc.date.available2012-09-05T05:22:19Z-
dc.date.issued2003en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 2003, v. 301 n. 3, p. 627-632en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/162684-
dc.description.abstractWe have constructed a 27-kDa hTERT C-terminal polypeptide (hTERTC27) devoid of domains required for telomerase activity and demonstrated that it is capable of nuclear translocation/telomere-end targeting. Here we showed that expression of a low level of hTERTC27 renders hTERT positive HeLa cells sensitive to H2O2-induced oxidative stress and subsequent cell senescence. The senescence-associated gene, the cyclin/cdk inhibitor p21 Waf1, was up-regulated. This occurs without changing the expression of endogenous hTERT, causing significant telomere shortening or inhibiting telomerase activity. Results from this study suggest for the first time that in addition to telomerase activity, the C-terminus of hTERT also plays a role in hTERT-mediated cellular resistance to oxidative stress. © 2003 Elsevier Science (USA). All rights reserved.en_HK
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.subjectHeLa cellsen_HK
dc.subjecthTERT C-terminusen_HK
dc.subjectOxidative stressen_HK
dc.subjectSenescenceen_HK
dc.subject.meshCell Agingen_US
dc.subject.meshClone Cellsen_US
dc.subject.meshCyclin-Dependent Kinase Inhibitor P21en_US
dc.subject.meshCyclins - Biosynthesisen_US
dc.subject.meshDna-Binding Proteinsen_US
dc.subject.meshHela Cellsen_US
dc.subject.meshHumansen_US
dc.subject.meshHydrogen Peroxide - Pharmacologyen_US
dc.subject.meshOxidants - Pharmacologyen_US
dc.subject.meshOxidative Stressen_US
dc.subject.meshPeptides - Genetics - Pharmacologyen_US
dc.subject.meshRecombinant Fusion Proteins - Pharmacologyen_US
dc.subject.meshTelomerase - Chemistry - Genetics - Metabolismen_US
dc.titleA human TERT C-terminal polypeptide sensitizes HeLa cells to H2O2-induced senescence without affecting telomerase enzymatic activityen_HK
dc.typeArticleen_HK
dc.identifier.emailNg, SSM: ssmng@hku.hken_HK
dc.identifier.emailWong, BCY: bcywong@hku.hken_HK
dc.identifier.emailLin, MCM: mcllin@hkucc.hku.hken_HK
dc.identifier.authorityNg, SSM=rp00767en_HK
dc.identifier.authorityWong, BCY=rp00429en_HK
dc.identifier.authorityLin, MCM=rp00746en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0006-291X(02)03049-8en_HK
dc.identifier.pmid12565825-
dc.identifier.scopuseid_2-s2.0-0037436268en_HK
dc.identifier.hkuros79129-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037436268&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume301en_HK
dc.identifier.issue3en_HK
dc.identifier.spage627en_HK
dc.identifier.epage632en_HK
dc.identifier.isiWOS:000181143800001-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHuang, JJ=7407194640en_HK
dc.identifier.scopusauthoridBai, YX=36127318700en_HK
dc.identifier.scopusauthoridHan, SW=7405942704en_HK
dc.identifier.scopusauthoridNg, SSM=7403358718en_HK
dc.identifier.scopusauthoridJing, DD=15026705900en_HK
dc.identifier.scopusauthoridWong, BCY=7402023340en_HK
dc.identifier.scopusauthoridHuang, CF=7406879416en_HK
dc.identifier.scopusauthoridKung, HF=7402514190en_HK
dc.identifier.scopusauthoridLin, MCM=7404816359en_HK

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