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Article: The shufflon of Salmonella enterica serovar Typhi regulates type IVB pilus-mediated bacterial self-association

TitleThe shufflon of Salmonella enterica serovar Typhi regulates type IVB pilus-mediated bacterial self-association
Authors
Issue Date2003
Citation
Infection And Immunity, 2003, v. 71 n. 3, p. 1141-1146 How to Cite?
AbstractPreviously, it was shown that type IVB pili encoded by the Salmonella enterica serovar Typhi pil operon are used to facilitate bacterial entry into human intestinal epithelial cells in vitro and that such entry is inhibited by purified prepilin (pre-PiIS) protein (X.-L. Zhang, I. S. M. Tsui, C. M. C. Yip, A. W. Y. Fung, D. K.-H. Wong, X. Dai, Y. Yang, J. Hackett, and C. Morris, Infect. Immun. 68:3067-3073, 2000). The pil operon concludes with a simple shufflon, and a recombinase gene product (Rci) inverts DNA in the C-terminal region of the pi/V gene to allow synthesis of two distinct PilV proteins, PilV1 and PilV2, which are presumptive minor pilus proteins. We show here that the type IVB pili mediate bacterial self-association, but only when the PilV1 and PilV2 proteins are not expressed. This may be achieved in wild-type serovar Typhi by rapid DNA inversion activity of the shufflon. We show that the inversion activity inhibits the expression of genes inserted between the 19-bp inverted repeats used for Rci-mediated recombination and that the activity of Rci increases when DNA is supercoiled. The data suggest that serovar Typhi self-associates under conditions (such as low oxygen tension in the gut) that favor DNA supercoiling. These results explain (i) the function of the serovar Typhi shufflon and (ii) why there are only two possible shufflon states, in contrast to the many possible states of other shufflon systems. The data further indicate that a very early step in serovar Typhi pathogenesis may be type IVB pilus-mediated self-association of bacteria in the anaerobic human small intestine prior to invasion of the human gut epithelium. The suggested type IVB pilus-dependent step in typhoid fever pathogenesis may partially explain the enhanced invasiveness of serovar Typhi for humans.
Persistent Identifierhttp://hdl.handle.net/10722/162676
ISSN
2015 Impact Factor: 3.603
2015 SCImago Journal Rankings: 2.342
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMorris, Cen_US
dc.contributor.authorYip, CMCen_US
dc.contributor.authorTsui, ISMen_US
dc.contributor.authorWong, DKHen_US
dc.contributor.authorHackett, Jen_US
dc.date.accessioned2012-09-05T05:22:15Z-
dc.date.available2012-09-05T05:22:15Z-
dc.date.issued2003en_US
dc.identifier.citationInfection And Immunity, 2003, v. 71 n. 3, p. 1141-1146en_US
dc.identifier.issn0019-9567en_US
dc.identifier.urihttp://hdl.handle.net/10722/162676-
dc.description.abstractPreviously, it was shown that type IVB pili encoded by the Salmonella enterica serovar Typhi pil operon are used to facilitate bacterial entry into human intestinal epithelial cells in vitro and that such entry is inhibited by purified prepilin (pre-PiIS) protein (X.-L. Zhang, I. S. M. Tsui, C. M. C. Yip, A. W. Y. Fung, D. K.-H. Wong, X. Dai, Y. Yang, J. Hackett, and C. Morris, Infect. Immun. 68:3067-3073, 2000). The pil operon concludes with a simple shufflon, and a recombinase gene product (Rci) inverts DNA in the C-terminal region of the pi/V gene to allow synthesis of two distinct PilV proteins, PilV1 and PilV2, which are presumptive minor pilus proteins. We show here that the type IVB pili mediate bacterial self-association, but only when the PilV1 and PilV2 proteins are not expressed. This may be achieved in wild-type serovar Typhi by rapid DNA inversion activity of the shufflon. We show that the inversion activity inhibits the expression of genes inserted between the 19-bp inverted repeats used for Rci-mediated recombination and that the activity of Rci increases when DNA is supercoiled. The data suggest that serovar Typhi self-associates under conditions (such as low oxygen tension in the gut) that favor DNA supercoiling. These results explain (i) the function of the serovar Typhi shufflon and (ii) why there are only two possible shufflon states, in contrast to the many possible states of other shufflon systems. The data further indicate that a very early step in serovar Typhi pathogenesis may be type IVB pilus-mediated self-association of bacteria in the anaerobic human small intestine prior to invasion of the human gut epithelium. The suggested type IVB pilus-dependent step in typhoid fever pathogenesis may partially explain the enhanced invasiveness of serovar Typhi for humans.en_US
dc.languageengen_US
dc.relation.ispartofInfection and Immunityen_US
dc.subject.meshBacterial Proteins - Genetics - Physiologyen_US
dc.subject.meshCell Lineen_US
dc.subject.meshDna, Superhelical - Chemistryen_US
dc.subject.meshFimbriae, Bacterial - Physiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshSalmonella Typhi - Genetics - Pathogenicityen_US
dc.subject.meshTranscription, Geneticen_US
dc.subject.meshTyphoid Fever - Etiologyen_US
dc.titleThe shufflon of Salmonella enterica serovar Typhi regulates type IVB pilus-mediated bacterial self-associationen_US
dc.typeArticleen_US
dc.identifier.emailWong, DKH:danywong@hku.hken_US
dc.identifier.authorityWong, DKH=rp00492en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1128/IAI.71.3.1141-1146.2003en_US
dc.identifier.pmid12595425-
dc.identifier.scopuseid_2-s2.0-0037369743en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037369743&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume71en_US
dc.identifier.issue3en_US
dc.identifier.spage1141en_US
dc.identifier.epage1146en_US
dc.identifier.isiWOS:000181270900013-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridMorris, C=7401472993en_US
dc.identifier.scopusauthoridYip, CMC=7101665509en_US
dc.identifier.scopusauthoridTsui, ISM=6602387676en_US
dc.identifier.scopusauthoridWong, DKH=7401535819en_US
dc.identifier.scopusauthoridHackett, J=24302040000en_US

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