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Article: Increased expression of G protein-coupled receptor kinases in cystic fibrosis lung

TitleIncreased expression of G protein-coupled receptor kinases in cystic fibrosis lung
Authors
Issue Date2002
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ejphar
Citation
European Journal Of Pharmacology, 2002, v. 436 n. 3, p. 165-172 How to Cite?
AbstractA reduction in airway β-adrenoceptor density has been reported in cystic fibrosis lung but the mechanism underlying this defect remains unclear. In this study, we have investigated whether the decrease in β2-adrenoceptor associates with altered G protein-coupled receptor kinase (GRK) levels. We assessed GRK activity by rhodopsin phosphorylation, and β2-adrenoceptor and GRK at the mRNA and protein levels by Northern and Western blotting in peripheral lung samples from normal donors and patients with cystic fibrosis. GRK activity was significantly increased in peripheral cystic fibrosis lung with parallel increases in GRK2/5 mRNAs and protein expression. Functionally, isoproterenol-stimulated adenylyl cyclase activity was also diminished by 65% in cystic fibrosis lung homogenates. These data suggest that the increase in GRK activity may be one of the mechanisms underlying alterations in the coupling between β2-adrenoceptor and adenylyl cyclase via G-protein and may thus contribute to the downregulation of β2-adrenoceptor in cystic fibrosis lung. © 2002 Elsevier Science B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/162652
ISSN
2015 Impact Factor: 2.73
2015 SCImago Journal Rankings: 1.115
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMak, JCWen_US
dc.contributor.authorChuang, TTen_US
dc.contributor.authorHarris, CAen_US
dc.contributor.authorBarnes, PJen_US
dc.date.accessioned2012-09-05T05:22:05Z-
dc.date.available2012-09-05T05:22:05Z-
dc.date.issued2002en_US
dc.identifier.citationEuropean Journal Of Pharmacology, 2002, v. 436 n. 3, p. 165-172en_US
dc.identifier.issn0014-2999en_US
dc.identifier.urihttp://hdl.handle.net/10722/162652-
dc.description.abstractA reduction in airway β-adrenoceptor density has been reported in cystic fibrosis lung but the mechanism underlying this defect remains unclear. In this study, we have investigated whether the decrease in β2-adrenoceptor associates with altered G protein-coupled receptor kinase (GRK) levels. We assessed GRK activity by rhodopsin phosphorylation, and β2-adrenoceptor and GRK at the mRNA and protein levels by Northern and Western blotting in peripheral lung samples from normal donors and patients with cystic fibrosis. GRK activity was significantly increased in peripheral cystic fibrosis lung with parallel increases in GRK2/5 mRNAs and protein expression. Functionally, isoproterenol-stimulated adenylyl cyclase activity was also diminished by 65% in cystic fibrosis lung homogenates. These data suggest that the increase in GRK activity may be one of the mechanisms underlying alterations in the coupling between β2-adrenoceptor and adenylyl cyclase via G-protein and may thus contribute to the downregulation of β2-adrenoceptor in cystic fibrosis lung. © 2002 Elsevier Science B.V. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ejpharen_US
dc.relation.ispartofEuropean Journal of Pharmacologyen_US
dc.subject.meshAdenylate Cyclase - Metabolismen_US
dc.subject.meshBlotting, Northernen_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshCyclic Amp-Dependent Protein Kinases - Genetics - Metabolismen_US
dc.subject.meshCystic Fibrosis - Enzymology - Geneticsen_US
dc.subject.meshG-Protein-Coupled Receptor Kinase 5en_US
dc.subject.meshGtp-Binding Proteins - Metabolismen_US
dc.subject.meshGene Expression Regulationen_US
dc.subject.meshHumansen_US
dc.subject.meshLung Diseases - Enzymology - Geneticsen_US
dc.subject.meshProtein-Serine-Threonine Kinases - Genetics - Metabolismen_US
dc.subject.meshRna, Messenger - Genetics - Metabolismen_US
dc.subject.meshReceptors, Adrenergic, Beta-2 - Genetics - Metabolismen_US
dc.subject.meshBeta-Adrenergic Receptor Kinasesen_US
dc.titleIncreased expression of G protein-coupled receptor kinases in cystic fibrosis lungen_US
dc.typeArticleen_US
dc.identifier.emailMak, JCW:judymak@hku.hken_US
dc.identifier.authorityMak, JCW=rp00352en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0014-2999(01)01625-9en_US
dc.identifier.pmid11858796-
dc.identifier.scopuseid_2-s2.0-0037006417en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037006417&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume436en_US
dc.identifier.issue3en_US
dc.identifier.spage165en_US
dc.identifier.epage172en_US
dc.identifier.isiWOS:000174129600003-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridMak, JCW=7103323094en_US
dc.identifier.scopusauthoridChuang, TT=7202737767en_US
dc.identifier.scopusauthoridHarris, CA=15823840800en_US
dc.identifier.scopusauthoridBarnes, PJ=36064679400en_US

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