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Article: Inhibitory effects of aspirin and indometacin on the growth of Helicobacter pylori in vitro

TitleInhibitory effects of aspirin and indometacin on the growth of Helicobacter pylori in vitro
Authors
KeywordsAmoxicillin
Aspirin
Clarithromycin
Helicobacter Pylori
Indometacin
Metronidazole
Non-Steroidal Anti-Inflammatory Drugs
Issue Date2002
PublisherBlackwell Publishing Asia. The Journal's web site is located at http://www.blackwellpublishing.com/journal.asp?ref=1443-9611&site=1
Citation
Chinese Journal Of Digestive Diseases, 2002, v. 3 n. 4, p. 172-177 How to Cite?
AbstractObjective: The interactions between non-steroidal anti-inflammatory drugs and Helicobacter pylori have not been sufficiently documented to date. The aim of this study was to investigate the possible effects of aspirin and indometacin on the growth of H. pylori and to determine the effects of aspirin on the susceptibility of H. pylori to some antimicrobials. Methods: Kinetic studies were performed by inoculating strains of H. pylori in brucella broth with different concentrations of aspirin and indometacin. Growth of bacteria in the broth was assessed spectrophotometrically and by viable colony counts after incubation for 24 and 48 h. Bacterial morphology was determined by Gram stain under light microscopy. The minimal inhibitory concentration (MIC) of aspirin and indometacin was determined by the standard agar dilution method. The MIC of amoxicillin, clarithromycin and metronidazole was measured in the presence and absence of aspirin by the E-test method. Results: Kinetic studies revealed that aspirin and indometacin inhibited the growth of H. pylori in a dose-dependent manner. The bactericidal activity of these agents was expressed by cell lysis. Aspirin at 400 μg/mL produced an almost 2-log decrease in the number of CFU/mL at 48 h. Similar inhibitory effects were obtained when 100 μg/ml, indometacin was tested. The MIC at which 90% of H. pylori was inhibited was 512 μg/mL and 128 μg/ml, for aspirin and indometacin, respectively. Increased susceptibility of H. pylori to amoxicillin, clarithromycin and metronidazole was found in the presence of aspirin. Conclusions: Aspirin and indometacin could significantly inhibit the growth of H. pylori when incubated in brucella broth in vitro. A subinhibitory concentration of aspirin enhanced the susceptibility of H. pylori to some antimicrobial agents.
Persistent Identifierhttp://hdl.handle.net/10722/162650
ISSN
References

 

DC FieldValueLanguage
dc.contributor.authorWang, WHen_US
dc.contributor.authorHu, FLen_US
dc.contributor.authorWong, BCYen_US
dc.contributor.authorBerg, DEen_US
dc.contributor.authorLam, SKen_US
dc.date.accessioned2012-09-05T05:22:04Z-
dc.date.available2012-09-05T05:22:04Z-
dc.date.issued2002en_US
dc.identifier.citationChinese Journal Of Digestive Diseases, 2002, v. 3 n. 4, p. 172-177en_US
dc.identifier.issn1443-9611en_US
dc.identifier.urihttp://hdl.handle.net/10722/162650-
dc.description.abstractObjective: The interactions between non-steroidal anti-inflammatory drugs and Helicobacter pylori have not been sufficiently documented to date. The aim of this study was to investigate the possible effects of aspirin and indometacin on the growth of H. pylori and to determine the effects of aspirin on the susceptibility of H. pylori to some antimicrobials. Methods: Kinetic studies were performed by inoculating strains of H. pylori in brucella broth with different concentrations of aspirin and indometacin. Growth of bacteria in the broth was assessed spectrophotometrically and by viable colony counts after incubation for 24 and 48 h. Bacterial morphology was determined by Gram stain under light microscopy. The minimal inhibitory concentration (MIC) of aspirin and indometacin was determined by the standard agar dilution method. The MIC of amoxicillin, clarithromycin and metronidazole was measured in the presence and absence of aspirin by the E-test method. Results: Kinetic studies revealed that aspirin and indometacin inhibited the growth of H. pylori in a dose-dependent manner. The bactericidal activity of these agents was expressed by cell lysis. Aspirin at 400 μg/mL produced an almost 2-log decrease in the number of CFU/mL at 48 h. Similar inhibitory effects were obtained when 100 μg/ml, indometacin was tested. The MIC at which 90% of H. pylori was inhibited was 512 μg/mL and 128 μg/ml, for aspirin and indometacin, respectively. Increased susceptibility of H. pylori to amoxicillin, clarithromycin and metronidazole was found in the presence of aspirin. Conclusions: Aspirin and indometacin could significantly inhibit the growth of H. pylori when incubated in brucella broth in vitro. A subinhibitory concentration of aspirin enhanced the susceptibility of H. pylori to some antimicrobial agents.en_US
dc.languageengen_US
dc.publisherBlackwell Publishing Asia. The Journal's web site is located at http://www.blackwellpublishing.com/journal.asp?ref=1443-9611&site=1en_US
dc.relation.ispartofChinese Journal of Digestive Diseasesen_US
dc.subjectAmoxicillinen_US
dc.subjectAspirinen_US
dc.subjectClarithromycinen_US
dc.subjectHelicobacter Pylorien_US
dc.subjectIndometacinen_US
dc.subjectMetronidazoleen_US
dc.subjectNon-Steroidal Anti-Inflammatory Drugsen_US
dc.titleInhibitory effects of aspirin and indometacin on the growth of Helicobacter pylori in vitroen_US
dc.typeArticleen_US
dc.identifier.emailCy Wong, B:bcywong@hku.hken_US
dc.identifier.authorityWong, BCY=rp00429en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1046/j.1443-9573.2002.00098.xen_US
dc.identifier.scopuseid_2-s2.0-0037002470en_US
dc.identifier.hkuros82285-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037002470&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume3en_US
dc.identifier.issue4en_US
dc.identifier.spage172en_US
dc.identifier.epage177en_US
dc.publisher.placeAustraliaen_US
dc.identifier.scopusauthoridWang, WH=23390847100en_US
dc.identifier.scopusauthoridHu, FL=7202526345en_US
dc.identifier.scopusauthoridCy Wong, B=7402023340en_US
dc.identifier.scopusauthoridBerg, DE=7202401139en_US
dc.identifier.scopusauthoridLam, SK=7402279473en_US
dc.customcontrol.immutablejt 131009-

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