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Article: Increased expression of survivin in gastric cancer patients and in first degree relatives

TitleIncreased expression of survivin in gastric cancer patients and in first degree relatives
Authors
Issue Date2002
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/bjc
Citation
British Journal Of Cancer, 2002, v. 87 n. 1, p. 91-97 How to Cite?
Abstract
Survivin was recently described as an apoptosis inhibitor. Its pathogenic role in gastric cancer is largely unknown. Expression of survivin in gastric cancer and non-cancer first-degree relatives, and its association with apoptosis and cyclo-oxygenase-2 expression was investigated. Fifty gastric cancer, 30 non-cancer first-degree relatives, 20 normal controls and five gastric cancer cell lines were studied. Survivin and cyclo-oxygenase-2 were evaluated by reverse transcriptase-polymerase chain reaction, immunohistochemistry and Western blot. Survivin expression was absent from normal gastric mucosa. All five cancer cell lines and 34 out of 50 (68%) human gastric cancer tissues expressed survivin mRNA. Survivin expression was less frequent (22%; P<0.001) in adjacent non-tumour gastric tissues. Immunohistochemistry and Western blot obtained similar findings. Gastric cancers with survivin expression displayed significantly reduced apoptosis (P=0.02), and associated with cyclo-oxygenase-2 overexpression at both mRNA (P=0.001) and protein levels (P=0.041). Moreover, survivin mRNA was detected in the gastric mucosa of eight (27%) non-cancer relatives. Expression in non-cancer patients showed positive correlation with H. pylori infection (P=0.004). This demonstrates the frequent expression of survivin in gastric cancer and in first-degree relatives. Co-expression of survivin and cyclo-oxygenase-2 may suggest multiple pathways contributing to the inhibition of apoptosis in gastric cancer. © 2002 Cancer Research UK.
Persistent Identifierhttp://hdl.handle.net/10722/162624
ISSN
2013 Impact Factor: 4.817
ISI Accession Number ID
References

 

Author Affiliations
  1. Prince of Wales Hospital Hong Kong
DC FieldValueLanguage
dc.contributor.authorYu, Jen_US
dc.contributor.authorLeung, WKen_US
dc.contributor.authorEbert, MPAen_US
dc.contributor.authorNg, EKWen_US
dc.contributor.authorGo, MYYen_US
dc.contributor.authorWang, HBen_US
dc.contributor.authorChung, SCSen_US
dc.contributor.authorMalfertheiner, Pen_US
dc.contributor.authorSung, JJYen_US
dc.date.accessioned2012-09-05T05:21:48Z-
dc.date.available2012-09-05T05:21:48Z-
dc.date.issued2002en_US
dc.identifier.citationBritish Journal Of Cancer, 2002, v. 87 n. 1, p. 91-97en_US
dc.identifier.issn0007-0920en_US
dc.identifier.urihttp://hdl.handle.net/10722/162624-
dc.description.abstractSurvivin was recently described as an apoptosis inhibitor. Its pathogenic role in gastric cancer is largely unknown. Expression of survivin in gastric cancer and non-cancer first-degree relatives, and its association with apoptosis and cyclo-oxygenase-2 expression was investigated. Fifty gastric cancer, 30 non-cancer first-degree relatives, 20 normal controls and five gastric cancer cell lines were studied. Survivin and cyclo-oxygenase-2 were evaluated by reverse transcriptase-polymerase chain reaction, immunohistochemistry and Western blot. Survivin expression was absent from normal gastric mucosa. All five cancer cell lines and 34 out of 50 (68%) human gastric cancer tissues expressed survivin mRNA. Survivin expression was less frequent (22%; P<0.001) in adjacent non-tumour gastric tissues. Immunohistochemistry and Western blot obtained similar findings. Gastric cancers with survivin expression displayed significantly reduced apoptosis (P=0.02), and associated with cyclo-oxygenase-2 overexpression at both mRNA (P=0.001) and protein levels (P=0.041). Moreover, survivin mRNA was detected in the gastric mucosa of eight (27%) non-cancer relatives. Expression in non-cancer patients showed positive correlation with H. pylori infection (P=0.004). This demonstrates the frequent expression of survivin in gastric cancer and in first-degree relatives. Co-expression of survivin and cyclo-oxygenase-2 may suggest multiple pathways contributing to the inhibition of apoptosis in gastric cancer. © 2002 Cancer Research UK.en_US
dc.languageengen_US
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/bjcen_US
dc.relation.ispartofBritish Journal of Canceren_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAged, 80 And Overen_US
dc.subject.meshApoptosisen_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshChromosomal Proteins, Non-Histone - Biosynthesisen_US
dc.subject.meshCyclooxygenase 2en_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Expression Regulation, Neoplasticen_US
dc.subject.meshGenetic Predisposition To Diseaseen_US
dc.subject.meshHelicobacter Infections - Complicationsen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshInhibitor Of Apoptosis Proteinsen_US
dc.subject.meshIsoenzymes - Biosynthesisen_US
dc.subject.meshMaleen_US
dc.subject.meshMembrane Proteinsen_US
dc.subject.meshMicrotubule-Associated Proteinsen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshNeoplasm Proteinsen_US
dc.subject.meshPedigreeen_US
dc.subject.meshProstaglandin-Endoperoxide Synthases - Biosynthesisen_US
dc.subject.meshRna, Messenger - Analysisen_US
dc.subject.meshStomach Neoplasms - Genetics - Physiopathologyen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.titleIncreased expression of survivin in gastric cancer patients and in first degree relativesen_US
dc.typeArticleen_US
dc.identifier.emailLeung, WK:waikleung@hku.hken_US
dc.identifier.authorityLeung, WK=rp01479en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1038/sj.bjc.6600421en_US
dc.identifier.pmid12085263en_US
dc.identifier.scopuseid_2-s2.0-0036648414en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036648414&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume87en_US
dc.identifier.issue1en_US
dc.identifier.spage91en_US
dc.identifier.epage97en_US
dc.identifier.isiWOS:000176878400017-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridYu, J=35351306800en_US
dc.identifier.scopusauthoridLeung, WK=7201504523en_US
dc.identifier.scopusauthoridEbert, MPA=35239660600en_US
dc.identifier.scopusauthoridNg, EKW=7201647539en_US
dc.identifier.scopusauthoridGo, MYY=7101882939en_US
dc.identifier.scopusauthoridWang, HB=8870507500en_US
dc.identifier.scopusauthoridChung, SCS=19642462800en_US
dc.identifier.scopusauthoridMalfertheiner, P=36048150200en_US
dc.identifier.scopusauthoridSung, JJY=35405352400en_US

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