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Article: Increased expression of survivin in gastric cancer patients and in first degree relatives
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TitleIncreased expression of survivin in gastric cancer patients and in first degree relatives
 
AuthorsYu, J1
Leung, WK1
Ebert, MPA1
Ng, EKW1
Go, MYY1
Wang, HB1
Chung, SCS1
Malfertheiner, P1
Sung, JJY1
 
Issue Date2002
 
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/bjc
 
CitationBritish Journal Of Cancer, 2002, v. 87 n. 1, p. 91-97 [How to Cite?]
DOI: http://dx.doi.org/10.1038/sj.bjc.6600421
 
AbstractSurvivin was recently described as an apoptosis inhibitor. Its pathogenic role in gastric cancer is largely unknown. Expression of survivin in gastric cancer and non-cancer first-degree relatives, and its association with apoptosis and cyclo-oxygenase-2 expression was investigated. Fifty gastric cancer, 30 non-cancer first-degree relatives, 20 normal controls and five gastric cancer cell lines were studied. Survivin and cyclo-oxygenase-2 were evaluated by reverse transcriptase-polymerase chain reaction, immunohistochemistry and Western blot. Survivin expression was absent from normal gastric mucosa. All five cancer cell lines and 34 out of 50 (68%) human gastric cancer tissues expressed survivin mRNA. Survivin expression was less frequent (22%; P<0.001) in adjacent non-tumour gastric tissues. Immunohistochemistry and Western blot obtained similar findings. Gastric cancers with survivin expression displayed significantly reduced apoptosis (P=0.02), and associated with cyclo-oxygenase-2 overexpression at both mRNA (P=0.001) and protein levels (P=0.041). Moreover, survivin mRNA was detected in the gastric mucosa of eight (27%) non-cancer relatives. Expression in non-cancer patients showed positive correlation with H. pylori infection (P=0.004). This demonstrates the frequent expression of survivin in gastric cancer and in first-degree relatives. Co-expression of survivin and cyclo-oxygenase-2 may suggest multiple pathways contributing to the inhibition of apoptosis in gastric cancer. © 2002 Cancer Research UK.
 
ISSN0007-0920
2012 Impact Factor: 5.082
2012 SCImago Journal Rankings: 2.311
 
DOIhttp://dx.doi.org/10.1038/sj.bjc.6600421
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorYu, J
 
dc.contributor.authorLeung, WK
 
dc.contributor.authorEbert, MPA
 
dc.contributor.authorNg, EKW
 
dc.contributor.authorGo, MYY
 
dc.contributor.authorWang, HB
 
dc.contributor.authorChung, SCS
 
dc.contributor.authorMalfertheiner, P
 
dc.contributor.authorSung, JJY
 
dc.date.accessioned2012-09-05T05:21:48Z
 
dc.date.available2012-09-05T05:21:48Z
 
dc.date.issued2002
 
dc.description.abstractSurvivin was recently described as an apoptosis inhibitor. Its pathogenic role in gastric cancer is largely unknown. Expression of survivin in gastric cancer and non-cancer first-degree relatives, and its association with apoptosis and cyclo-oxygenase-2 expression was investigated. Fifty gastric cancer, 30 non-cancer first-degree relatives, 20 normal controls and five gastric cancer cell lines were studied. Survivin and cyclo-oxygenase-2 were evaluated by reverse transcriptase-polymerase chain reaction, immunohistochemistry and Western blot. Survivin expression was absent from normal gastric mucosa. All five cancer cell lines and 34 out of 50 (68%) human gastric cancer tissues expressed survivin mRNA. Survivin expression was less frequent (22%; P<0.001) in adjacent non-tumour gastric tissues. Immunohistochemistry and Western blot obtained similar findings. Gastric cancers with survivin expression displayed significantly reduced apoptosis (P=0.02), and associated with cyclo-oxygenase-2 overexpression at both mRNA (P=0.001) and protein levels (P=0.041). Moreover, survivin mRNA was detected in the gastric mucosa of eight (27%) non-cancer relatives. Expression in non-cancer patients showed positive correlation with H. pylori infection (P=0.004). This demonstrates the frequent expression of survivin in gastric cancer and in first-degree relatives. Co-expression of survivin and cyclo-oxygenase-2 may suggest multiple pathways contributing to the inhibition of apoptosis in gastric cancer. © 2002 Cancer Research UK.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationBritish Journal Of Cancer, 2002, v. 87 n. 1, p. 91-97 [How to Cite?]
DOI: http://dx.doi.org/10.1038/sj.bjc.6600421
 
dc.identifier.doihttp://dx.doi.org/10.1038/sj.bjc.6600421
 
dc.identifier.epage97
 
dc.identifier.issn0007-0920
2012 Impact Factor: 5.082
2012 SCImago Journal Rankings: 2.311
 
dc.identifier.issue1
 
dc.identifier.pmid12085263
 
dc.identifier.scopuseid_2-s2.0-0036648414
 
dc.identifier.spage91
 
dc.identifier.urihttp://hdl.handle.net/10722/162624
 
dc.identifier.volume87
 
dc.languageeng
 
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/bjc
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofBritish Journal of Cancer
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAdult
 
dc.subject.meshAged
 
dc.subject.meshAged, 80 And Over
 
dc.subject.meshApoptosis
 
dc.subject.meshBlotting, Western
 
dc.subject.meshChromosomal Proteins, Non-Histone - Biosynthesis
 
dc.subject.meshCyclooxygenase 2
 
dc.subject.meshFemale
 
dc.subject.meshGene Expression Regulation, Neoplastic
 
dc.subject.meshGenetic Predisposition To Disease
 
dc.subject.meshHelicobacter Infections - Complications
 
dc.subject.meshHumans
 
dc.subject.meshImmunohistochemistry
 
dc.subject.meshInhibitor Of Apoptosis Proteins
 
dc.subject.meshIsoenzymes - Biosynthesis
 
dc.subject.meshMale
 
dc.subject.meshMembrane Proteins
 
dc.subject.meshMicrotubule-Associated Proteins
 
dc.subject.meshMiddle Aged
 
dc.subject.meshNeoplasm Proteins
 
dc.subject.meshPedigree
 
dc.subject.meshProstaglandin-Endoperoxide Synthases - Biosynthesis
 
dc.subject.meshRna, Messenger - Analysis
 
dc.subject.meshStomach Neoplasms - Genetics - Physiopathology
 
dc.subject.meshTumor Cells, Cultured
 
dc.titleIncreased expression of survivin in gastric cancer patients and in first degree relatives
 
dc.typeArticle
 
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Author Affiliations
  1. Prince of Wales Hospital Hong Kong