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Article: Helicobacter pylori impairs DNA mismatch repair in gastric epithelial cells

TitleHelicobacter pylori impairs DNA mismatch repair in gastric epithelial cells
Authors
Issue Date2002
PublisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/gastro
Citation
Gastroenterology, 2002, v. 123 n. 2, p. 542-553 How to Cite?
AbstractBackground & Aims: Helicobacter pylori infection is a major gastric cancer risk factor. H. pylori gastritis occurs more frequently in individuals with microsatellite instability-positive than those with microsatellite instability-negative gastric cancers, raising the possibility that H. pylori infection affects DNA mismatch repair (MMR). The aim of this study was to determine the effect of H. pylori on the expression of DNA MMR proteins and RNA in gastric epithelial cells. Methods: Gastric cancer cell lines were cocultured with H. pylori, bacterial extracts, and Campylobacterjejuni or Escherichia coli. MutS (hMSH2 and hMSH6) and MutL (hMLH1, hPMS2, and hPMS1) DNA MMR protein and RNA levels were determined. Results: All cell lines examined showed decreased levels of MutS and MutL DNA MMR proteins in a dose-dependent manner after coculture with H. pylori strains. The reduction in DNA MMR protein levels was caused by heat-sensitive H. pylori products. The levels of DNA MMR proteins were affected by C. jejuni but not by E. coli. RNA levels of hMSH2 and hMSH6 were also reduced after exposure to H. pylori. Conclusions: H. pylori infection of gastric epithelial cells leads to a decrease in DNA MMR proteins that is at least in part related to an H. pylori-induced decrease in messenger RNA levels of repair genes. These data suggest that H. pylori infection might lead to a deficiency of DNA MMR in gastric epithelial cells that may increase the risk of mutation accumulation in gastric mucosa cells and the risk of gastric cancer during chronic H. pylori infection.
Persistent Identifierhttp://hdl.handle.net/10722/162604
ISSN
2015 Impact Factor: 18.187
2015 SCImago Journal Rankings: 7.170
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKim, JJen_US
dc.contributor.authorTao, Hen_US
dc.contributor.authorCarloni, Een_US
dc.contributor.authorLeung, WKen_US
dc.contributor.authorGraham, DYen_US
dc.contributor.authorSepulveda, ARen_US
dc.date.accessioned2012-09-05T05:21:35Z-
dc.date.available2012-09-05T05:21:35Z-
dc.date.issued2002en_US
dc.identifier.citationGastroenterology, 2002, v. 123 n. 2, p. 542-553en_US
dc.identifier.issn0016-5085en_US
dc.identifier.urihttp://hdl.handle.net/10722/162604-
dc.description.abstractBackground & Aims: Helicobacter pylori infection is a major gastric cancer risk factor. H. pylori gastritis occurs more frequently in individuals with microsatellite instability-positive than those with microsatellite instability-negative gastric cancers, raising the possibility that H. pylori infection affects DNA mismatch repair (MMR). The aim of this study was to determine the effect of H. pylori on the expression of DNA MMR proteins and RNA in gastric epithelial cells. Methods: Gastric cancer cell lines were cocultured with H. pylori, bacterial extracts, and Campylobacterjejuni or Escherichia coli. MutS (hMSH2 and hMSH6) and MutL (hMLH1, hPMS2, and hPMS1) DNA MMR protein and RNA levels were determined. Results: All cell lines examined showed decreased levels of MutS and MutL DNA MMR proteins in a dose-dependent manner after coculture with H. pylori strains. The reduction in DNA MMR protein levels was caused by heat-sensitive H. pylori products. The levels of DNA MMR proteins were affected by C. jejuni but not by E. coli. RNA levels of hMSH2 and hMSH6 were also reduced after exposure to H. pylori. Conclusions: H. pylori infection of gastric epithelial cells leads to a decrease in DNA MMR proteins that is at least in part related to an H. pylori-induced decrease in messenger RNA levels of repair genes. These data suggest that H. pylori infection might lead to a deficiency of DNA MMR in gastric epithelial cells that may increase the risk of mutation accumulation in gastric mucosa cells and the risk of gastric cancer during chronic H. pylori infection.en_US
dc.languageengen_US
dc.publisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/gastroen_US
dc.relation.ispartofGastroenterologyen_US
dc.subject.meshAdaptor Proteins, Signal Transducingen_US
dc.subject.meshBase Pair Mismatchen_US
dc.subject.meshCarrier Proteinsen_US
dc.subject.meshDna Repairen_US
dc.subject.meshDna-Binding Proteins - Analysisen_US
dc.subject.meshGastric Mucosa - Metabolismen_US
dc.subject.meshHelicobacter Pylori - Pathogenicityen_US
dc.subject.meshHumansen_US
dc.subject.meshMuts Homolog 2 Proteinen_US
dc.subject.meshNeoplasm Proteins - Analysisen_US
dc.subject.meshNuclear Proteinsen_US
dc.subject.meshProliferating Cell Nuclear Antigen - Analysisen_US
dc.subject.meshProto-Oncogene Proteins - Analysisen_US
dc.subject.meshStomach Neoplasms - Etiologyen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.titleHelicobacter pylori impairs DNA mismatch repair in gastric epithelial cellsen_US
dc.typeArticleen_US
dc.identifier.emailLeung, WK:waikleung@hku.hken_US
dc.identifier.authorityLeung, WK=rp01479en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1053/gast.2002.34751en_US
dc.identifier.pmid12145807-
dc.identifier.scopuseid_2-s2.0-0036320505en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036320505&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume123en_US
dc.identifier.issue2en_US
dc.identifier.spage542en_US
dc.identifier.epage553en_US
dc.identifier.isiWOS:000177085400018-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridKim, JJ=36067967700en_US
dc.identifier.scopusauthoridTao, H=36937832300en_US
dc.identifier.scopusauthoridCarloni, E=6603188197en_US
dc.identifier.scopusauthoridLeung, WK=7201504523en_US
dc.identifier.scopusauthoridGraham, DY=7403477677en_US
dc.identifier.scopusauthoridSepulveda, AR=35500186600en_US

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