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Article: Intracellular antibody capture technology: Application to selection of intracellular antibodies recognising the BCR-ABL oncogenic protein

TitleIntracellular antibody capture technology: Application to selection of intracellular antibodies recognising the BCR-ABL oncogenic protein
Authors
Issue Date2002
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/jmb
Citation
Journal Of Molecular Biology, 2002, v. 317 n. 1, p. 85-94 How to Cite?
AbstractThe expression of antibodies inside cells to ablate protein function has the potential for disease therapy and for target validation in functional genomics. However, due to inefficient expression or folding, only a few antibodies or antibody fragments, usually as single-chain Fv antibody fragments (scFv), bind their antigens in an intracellular environment. We have established a genetic-selection technology (intracellular antibody capture, IAC) to facilitate the isolation of functional intracellular scFv from a diverse repertoire. This approach comprises an in vitro library screen with scFv-expressing bacteriophage, employing bacterially expressed antigen, followed by a yeast in vivo antibody-antigen interaction screen of the sub-library of in vitro scFv antigen-binders. Accordingly, we have isolated panels of scFv that bind intracellularly to the BCR or the ABL parts of the BCR-ABL oncogenic protein. Sequence analysis of the intracellular antibody scFv panels revealed a sequence conservation indicating an intracellular antibody consensus for both VH and VL, which could form the basis for the de novo synthesis of intracellular antibody libraries to be used with intracellular antibody-capture technology. © 2002 Elsevier Science Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/162600
ISSN
2015 Impact Factor: 4.517
2015 SCImago Journal Rankings: 3.002
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTse, Een_US
dc.contributor.authorLobato, MNen_US
dc.contributor.authorForster, Aen_US
dc.contributor.authorTanaka, Ten_US
dc.contributor.authorChung, GTYen_US
dc.contributor.authorRabbitts, THen_US
dc.date.accessioned2012-09-05T05:21:34Z-
dc.date.available2012-09-05T05:21:34Z-
dc.date.issued2002en_US
dc.identifier.citationJournal Of Molecular Biology, 2002, v. 317 n. 1, p. 85-94en_US
dc.identifier.issn0022-2836en_US
dc.identifier.urihttp://hdl.handle.net/10722/162600-
dc.description.abstractThe expression of antibodies inside cells to ablate protein function has the potential for disease therapy and for target validation in functional genomics. However, due to inefficient expression or folding, only a few antibodies or antibody fragments, usually as single-chain Fv antibody fragments (scFv), bind their antigens in an intracellular environment. We have established a genetic-selection technology (intracellular antibody capture, IAC) to facilitate the isolation of functional intracellular scFv from a diverse repertoire. This approach comprises an in vitro library screen with scFv-expressing bacteriophage, employing bacterially expressed antigen, followed by a yeast in vivo antibody-antigen interaction screen of the sub-library of in vitro scFv antigen-binders. Accordingly, we have isolated panels of scFv that bind intracellularly to the BCR or the ABL parts of the BCR-ABL oncogenic protein. Sequence analysis of the intracellular antibody scFv panels revealed a sequence conservation indicating an intracellular antibody consensus for both VH and VL, which could form the basis for the de novo synthesis of intracellular antibody libraries to be used with intracellular antibody-capture technology. © 2002 Elsevier Science Ltd.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/jmben_US
dc.relation.ispartofJournal of Molecular Biologyen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies - Chemistry - Genetics - Immunologyen_US
dc.subject.meshAntibody Specificity - Immunologyen_US
dc.subject.meshAntigen-Antibody Reactions - Immunologyen_US
dc.subject.meshBacteriophages - Geneticsen_US
dc.subject.meshBinding Sites, Antibody - Immunologyen_US
dc.subject.meshCho Cellsen_US
dc.subject.meshCricetinaeen_US
dc.subject.meshFusion Proteins, Bcr-Abl - Genetics - Immunologyen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoglobulin Fab Fragments - Chemistry - Genetics - Immunologyen_US
dc.subject.meshImmunoglobulin Variable Region - Chemistry - Genetics - Immunologyen_US
dc.subject.meshIntracellular Fluid - Immunologyen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPeptide Libraryen_US
dc.subject.meshProtein-Tyrosine Kinasesen_US
dc.subject.meshProto-Oncogene Proteins - Genetics - Immunologyen_US
dc.subject.meshProto-Oncogene Proteins C-Abl - Genetics - Immunologyen_US
dc.subject.meshProto-Oncogene Proteins C-Bcren_US
dc.subject.meshRecombinant Proteins - Chemistry - Genetics - Immunologyen_US
dc.subject.meshSequence Alignmenten_US
dc.titleIntracellular antibody capture technology: Application to selection of intracellular antibodies recognising the BCR-ABL oncogenic proteinen_US
dc.typeArticleen_US
dc.identifier.emailTse, E:ewctse@hku.hken_US
dc.identifier.authorityTse, E=rp00471en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1006/jmbi.2002.5403en_US
dc.identifier.pmid11916380-
dc.identifier.scopuseid_2-s2.0-0036299007en_US
dc.identifier.hkuros77705-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036299007&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume317en_US
dc.identifier.issue1en_US
dc.identifier.spage85en_US
dc.identifier.epage94en_US
dc.identifier.isiWOS:000174613300005-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridTse, E=7005019454en_US
dc.identifier.scopusauthoridLobato, MN=35870245400en_US
dc.identifier.scopusauthoridForster, A=7201638425en_US
dc.identifier.scopusauthoridTanaka, T=7406725547en_US
dc.identifier.scopusauthoridChung, GTY=9246670000en_US
dc.identifier.scopusauthoridRabbitts, TH=7103136845en_US

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