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Article: Detection of gene promoter hypermethylation in the tumor and serum of patients with gastric carcinoma

TitleDetection of gene promoter hypermethylation in the tumor and serum of patients with gastric carcinoma
Authors
Issue Date2002
Citation
Clinical Cancer Research, 2002, v. 8 n. 6, p. 1761-1766 How to Cite?
AbstractPurpose: Aberrant promoter methylation, an alternative mechanism for gene silencing, is frequently detected in gastric cancer. We studied the feasibility of detecting aberrant methylation in serum of gastric cancer patients. Experimental Design: Patients (54) with gastric adenocarcinoma were studied. The tumor and the paired serum were examined for aberrant methylation in DAP-kinase, E-cadherin, GSTP1, p15, and p16 by methylation-specific PCR. Serum from 30 age-matched noncancer patients was used as control. Results: Promoter methylation in DAP-kinase, Ecadherin, GSTP1, p15, and p16 were detected in 70.3, 75.9, 18.5, 68.5, and 66.7% of primary tumor. In serum of gastric cancer patients, methylation in DAP-kinase, Ecadherin, GSTP1, p15, and p16 were detected in 48.1, 57.4, 14.8, 55.6, and 51.9%, respectively. None of the control serum showed aberrant methylation. Aberrant methylation in serum DNA was all accompanied with methylation in the corresponding tumor samples. In general, >60% of serum from cancers with aberrant methylation demonstrated these epigenetic alterations. Conclusion: Our findings suggest that aberrant promoter methylation in serum can be detected in a substantial proportion of gastric cancer patients, and this strategy should be evaluated in the screening and surveillance of gastric cancer.
Persistent Identifierhttp://hdl.handle.net/10722/162599
ISSN
2015 Impact Factor: 8.738
2015 SCImago Journal Rankings: 5.314
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLee, TLen_US
dc.contributor.authorLeung, WKen_US
dc.contributor.authorChan, MWYen_US
dc.contributor.authorNg, EKWen_US
dc.contributor.authorTong, JHMen_US
dc.contributor.authorLo, KWen_US
dc.contributor.authorChung, SCSen_US
dc.contributor.authorSung, JJYen_US
dc.contributor.authorTo, KFen_US
dc.date.accessioned2012-09-05T05:21:34Z-
dc.date.available2012-09-05T05:21:34Z-
dc.date.issued2002en_US
dc.identifier.citationClinical Cancer Research, 2002, v. 8 n. 6, p. 1761-1766en_US
dc.identifier.issn1078-0432en_US
dc.identifier.urihttp://hdl.handle.net/10722/162599-
dc.description.abstractPurpose: Aberrant promoter methylation, an alternative mechanism for gene silencing, is frequently detected in gastric cancer. We studied the feasibility of detecting aberrant methylation in serum of gastric cancer patients. Experimental Design: Patients (54) with gastric adenocarcinoma were studied. The tumor and the paired serum were examined for aberrant methylation in DAP-kinase, E-cadherin, GSTP1, p15, and p16 by methylation-specific PCR. Serum from 30 age-matched noncancer patients was used as control. Results: Promoter methylation in DAP-kinase, Ecadherin, GSTP1, p15, and p16 were detected in 70.3, 75.9, 18.5, 68.5, and 66.7% of primary tumor. In serum of gastric cancer patients, methylation in DAP-kinase, Ecadherin, GSTP1, p15, and p16 were detected in 48.1, 57.4, 14.8, 55.6, and 51.9%, respectively. None of the control serum showed aberrant methylation. Aberrant methylation in serum DNA was all accompanied with methylation in the corresponding tumor samples. In general, >60% of serum from cancers with aberrant methylation demonstrated these epigenetic alterations. Conclusion: Our findings suggest that aberrant promoter methylation in serum can be detected in a substantial proportion of gastric cancer patients, and this strategy should be evaluated in the screening and surveillance of gastric cancer.en_US
dc.languageengen_US
dc.relation.ispartofClinical Cancer Researchen_US
dc.subject.meshAdenocarcinoma - Blood - Metabolism - Pathologyen_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAged, 80 And Overen_US
dc.subject.meshApoptosis Regulatory Proteinsen_US
dc.subject.meshCadherins - Metabolismen_US
dc.subject.meshCalcium-Calmodulin-Dependent Protein Kinases - Metabolismen_US
dc.subject.meshCase-Control Studiesen_US
dc.subject.meshCell Cycle Proteinsen_US
dc.subject.meshCyclin-Dependent Kinase Inhibitor P15en_US
dc.subject.meshCyclin-Dependent Kinase Inhibitor P16 - Metabolismen_US
dc.subject.meshDna Methylationen_US
dc.subject.meshDna, Neoplasm - Blooden_US
dc.subject.meshGlutathione S-Transferase Pien_US
dc.subject.meshGlutathione Transferase - Metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshIsoenzymes - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshNeoplasm Proteins - Metabolismen_US
dc.subject.meshNeoplasm Stagingen_US
dc.subject.meshPromoter Regions, Geneticen_US
dc.subject.meshStomach Neoplasms - Blood - Metabolism - Pathologyen_US
dc.subject.meshTranscription Factors - Metabolismen_US
dc.subject.meshTumor Suppressor Proteinsen_US
dc.titleDetection of gene promoter hypermethylation in the tumor and serum of patients with gastric carcinomaen_US
dc.typeArticleen_US
dc.identifier.emailLeung, WK:waikleung@hku.hken_US
dc.identifier.authorityLeung, WK=rp01479en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid12060614-
dc.identifier.scopuseid_2-s2.0-0036284631en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036284631&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume8en_US
dc.identifier.issue6en_US
dc.identifier.spage1761en_US
dc.identifier.epage1766en_US
dc.identifier.isiWOS:000176141900011-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLee, TL=35292432600en_US
dc.identifier.scopusauthoridLeung, WK=7201504523en_US
dc.identifier.scopusauthoridChan, MWY=7402597788en_US
dc.identifier.scopusauthoridNg, EKW=7201647539en_US
dc.identifier.scopusauthoridTong, JHM=7202724564en_US
dc.identifier.scopusauthoridLo, KW=7402101603en_US
dc.identifier.scopusauthoridChung, SCS=19642462800en_US
dc.identifier.scopusauthoridSung, JJY=35405352400en_US
dc.identifier.scopusauthoridTo, KF=7101911940en_US

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