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Article: Postnatal changes in β-adrenoceptors in the lung and the effect of hypoxia induced pulmonary hypertension of the newborn

TitlePostnatal changes in β-adrenoceptors in the lung and the effect of hypoxia induced pulmonary hypertension of the newborn
Authors
Issue Date2002
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0007-1188&site=1
Citation
British Journal Of Pharmacology, 2002, v. 135 n. 6, p. 1415-1424 How to Cite?
Abstract1. β-adrenoceptor activation leads to pulmonary vasodilatation. The increase in circulating catecholamines at birth may assist the postnatal fall in vascular resistance by their activation. To study β 1- and β 2-adrenoceptors during postnatal adaptation, we used [ 125I]-iodocyanopindolol (ICYP) binding to lung membranes and sections to quantify and locate the binding sites in piglets from birth to 14 days of age and compared them with those in adult pigs. In addition, pulmonary hypertension was induced in newborn piglets by hypobaric hypoxia. 2. In lung membranes the equilibrium dissociation constant (K d) did not change with age for total β-adrenoceptors or for β 2-adrenoceptors, but there was a significant increase in maximum binding sites (B max) between birth and 3 days of age. On tissue sections, B max increased between 3 days and adulthood with no change in K d. 3. Binding sites of β 1- and β 2-adrenoceptors were localized to the bronchial epithelium, to endothelium of extra- and intra-pulmonary arteries and to lung parenchyma. Total β-adrenoceptor density increased with age at all locations (P <0.05-0.01). At birth intrapulmonary arteries showed no binding, β 2-adrenoceptors appeared on day 1 and increased up to 14 days of age. β 1-adrenoceptors appeared by 3 days of age and increased with age. 4. Hypobaric hypoxia from birth led to attenuation in the normal postnatal increase in receptor number, but hypoxia from 3-6 days did not decrease receptor density. 5. The normal postnatal increase in β-adrenoceptors suggests a potential for catecholamine induced dilatation in the lung during adaptation which is attenuated in pulmonary hypertension.
Persistent Identifierhttp://hdl.handle.net/10722/162594
ISSN
2015 Impact Factor: 5.259
2015 SCImago Journal Rankings: 2.368
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHislop, AAen_US
dc.contributor.authorMak, JCWen_US
dc.contributor.authorKelly, Den_US
dc.contributor.authorReader, JAen_US
dc.contributor.authorBarnes, PJen_US
dc.contributor.authorHaworth, SGen_US
dc.date.accessioned2012-09-05T05:21:32Z-
dc.date.available2012-09-05T05:21:32Z-
dc.date.issued2002en_US
dc.identifier.citationBritish Journal Of Pharmacology, 2002, v. 135 n. 6, p. 1415-1424en_US
dc.identifier.issn0007-1188en_US
dc.identifier.urihttp://hdl.handle.net/10722/162594-
dc.description.abstract1. β-adrenoceptor activation leads to pulmonary vasodilatation. The increase in circulating catecholamines at birth may assist the postnatal fall in vascular resistance by their activation. To study β 1- and β 2-adrenoceptors during postnatal adaptation, we used [ 125I]-iodocyanopindolol (ICYP) binding to lung membranes and sections to quantify and locate the binding sites in piglets from birth to 14 days of age and compared them with those in adult pigs. In addition, pulmonary hypertension was induced in newborn piglets by hypobaric hypoxia. 2. In lung membranes the equilibrium dissociation constant (K d) did not change with age for total β-adrenoceptors or for β 2-adrenoceptors, but there was a significant increase in maximum binding sites (B max) between birth and 3 days of age. On tissue sections, B max increased between 3 days and adulthood with no change in K d. 3. Binding sites of β 1- and β 2-adrenoceptors were localized to the bronchial epithelium, to endothelium of extra- and intra-pulmonary arteries and to lung parenchyma. Total β-adrenoceptor density increased with age at all locations (P <0.05-0.01). At birth intrapulmonary arteries showed no binding, β 2-adrenoceptors appeared on day 1 and increased up to 14 days of age. β 1-adrenoceptors appeared by 3 days of age and increased with age. 4. Hypobaric hypoxia from birth led to attenuation in the normal postnatal increase in receptor number, but hypoxia from 3-6 days did not decrease receptor density. 5. The normal postnatal increase in β-adrenoceptors suggests a potential for catecholamine induced dilatation in the lung during adaptation which is attenuated in pulmonary hypertension.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0007-1188&site=1en_US
dc.relation.ispartofBritish Journal of Pharmacologyen_US
dc.subject.meshAging - Metabolism - Physiologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnimals, Newborn - Physiologyen_US
dc.subject.meshAnoxia - Metabolismen_US
dc.subject.meshBinding Sites - Physiologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshHypertension, Pulmonary - Etiology - Metabolismen_US
dc.subject.meshLung - Growth & Development - Metabolismen_US
dc.subject.meshPregnancyen_US
dc.subject.meshReceptors, Adrenergic, Beta - Metabolismen_US
dc.subject.meshSwineen_US
dc.titlePostnatal changes in β-adrenoceptors in the lung and the effect of hypoxia induced pulmonary hypertension of the newbornen_US
dc.typeArticleen_US
dc.identifier.emailMak, JCW:judymak@hku.hken_US
dc.identifier.authorityMak, JCW=rp00352en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1038/sj.bjp.0704597-
dc.identifier.pmid11906954-
dc.identifier.scopuseid_2-s2.0-0036217752en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036217752&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume135en_US
dc.identifier.issue6en_US
dc.identifier.spage1415en_US
dc.identifier.epage1424en_US
dc.identifier.isiWOS:000174644400011-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridHislop, AA=7005801811en_US
dc.identifier.scopusauthoridMak, JCW=7103323094en_US
dc.identifier.scopusauthoridKelly, D=7403136812en_US
dc.identifier.scopusauthoridReader, JA=55160661300en_US
dc.identifier.scopusauthoridBarnes, PJ=36064679400en_US
dc.identifier.scopusauthoridHaworth, SG=35513064700en_US

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