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Article: Identification of novel putative membrane proteins selectively expressed during adipose conversion of 3T3-L1 cells

TitleIdentification of novel putative membrane proteins selectively expressed during adipose conversion of 3T3-L1 cells
Authors
KeywordsAdipogenesis
Insulin
Membrane protein
Metabolism
Proteomics
Issue Date2002
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 2002, v. 293 n. 4, p. 1161-1167 How to Cite?
AbstractFat tissue plays a critical role in the regulation of energy metabolism. Here we report the proteomic identification of a novel fat tissue-specific low molecular weight protein (Falp) which responds to insulin. Falp is preferentially expressed in adipocytes but not in preadipocytes, as shown by two-dimensional gel electrophoresis. Northern blot analysis shows that the Falp gene is predominantly expressed in brown and white fat tissues, but not in any other tissues examined. Human homologs of mouse Falp are found to exist as two alternatively spliced isoforms, which share the same N-terminus but have different C-termini. Both human and mouse Falp contain a conserved putative transmembrane domain. Immunofluorescent analyses of 3T3-L1 adipocytes show that Falp protein strictly localizes at a compact perinuclear membrane compartment. Treatment of cells with insulin induces the redistribution of Falp into numerous discrete spotty structures spreading throughout the cytoplasm. Whereas the function of Falp is currently unclear, its tissue specific expression and the responsiveness to insulin suggest that Falp might be involved in a process specifically restricted to adipose tissue function, such as vesicular transport and protein secretion. © 2002 Elsevier Science (USA). All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/162574
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorXu, Aen_HK
dc.contributor.authorChoi, KLen_HK
dc.contributor.authorWang, Yuen_HK
dc.contributor.authorPermana, PAen_HK
dc.contributor.authorYi Xu, Len_HK
dc.contributor.authorBogardus, Cen_HK
dc.contributor.authorCooper, GJSen_HK
dc.date.accessioned2012-09-05T05:21:20Z-
dc.date.available2012-09-05T05:21:20Z-
dc.date.issued2002en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 2002, v. 293 n. 4, p. 1161-1167en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/162574-
dc.description.abstractFat tissue plays a critical role in the regulation of energy metabolism. Here we report the proteomic identification of a novel fat tissue-specific low molecular weight protein (Falp) which responds to insulin. Falp is preferentially expressed in adipocytes but not in preadipocytes, as shown by two-dimensional gel electrophoresis. Northern blot analysis shows that the Falp gene is predominantly expressed in brown and white fat tissues, but not in any other tissues examined. Human homologs of mouse Falp are found to exist as two alternatively spliced isoforms, which share the same N-terminus but have different C-termini. Both human and mouse Falp contain a conserved putative transmembrane domain. Immunofluorescent analyses of 3T3-L1 adipocytes show that Falp protein strictly localizes at a compact perinuclear membrane compartment. Treatment of cells with insulin induces the redistribution of Falp into numerous discrete spotty structures spreading throughout the cytoplasm. Whereas the function of Falp is currently unclear, its tissue specific expression and the responsiveness to insulin suggest that Falp might be involved in a process specifically restricted to adipose tissue function, such as vesicular transport and protein secretion. © 2002 Elsevier Science (USA). All rights reserved.en_HK
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.subjectAdipogenesisen_HK
dc.subjectInsulinen_HK
dc.subjectMembrane proteinen_HK
dc.subjectMetabolismen_HK
dc.subjectProteomicsen_HK
dc.subject.mesh3T3 Cellsen_US
dc.subject.meshAdipocytes - Metabolismen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBlotting, Northernen_US
dc.subject.meshCos Cellsen_US
dc.subject.meshCell Differentiationen_US
dc.subject.meshCell Membrane - Metabolismen_US
dc.subject.meshChromatography, High Pressure Liquiden_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshDna, Complementary - Metabolismen_US
dc.subject.meshElectrophoresis, Gel, Two-Dimensionalen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshInsulin - Pharmacologyen_US
dc.subject.meshMembrane Proteins - Biosynthesis - Chemistry - Physiologyen_US
dc.subject.meshMiceen_US
dc.subject.meshModels, Geneticen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshProtein Isoformsen_US
dc.subject.meshProtein Structure, Tertiaryen_US
dc.subject.meshRna, Messenger - Metabolismen_US
dc.subject.meshTime Factorsen_US
dc.subject.meshTissue Distributionen_US
dc.titleIdentification of novel putative membrane proteins selectively expressed during adipose conversion of 3T3-L1 cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailXu, A: amxu@hkucc.hku.hken_HK
dc.identifier.emailWang, Yu: yuwanghk@hku.hken_HK
dc.identifier.authorityXu, A=rp00485en_HK
dc.identifier.authorityWang, Yu=rp00239en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0006-291X(02)00354-6en_HK
dc.identifier.pmid12054497-
dc.identifier.scopuseid_2-s2.0-0036076186en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036076186&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume293en_HK
dc.identifier.issue4en_HK
dc.identifier.spage1161en_HK
dc.identifier.epage1167en_HK
dc.identifier.isiWOS:000175911900003-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridXu, A=7202655409en_HK
dc.identifier.scopusauthoridChoi, KL=7403949320en_HK
dc.identifier.scopusauthoridWang, Yu=34973733700en_HK
dc.identifier.scopusauthoridPermana, PA=6603873556en_HK
dc.identifier.scopusauthoridYi Xu, L=15827160000en_HK
dc.identifier.scopusauthoridBogardus, C=7102903658en_HK
dc.identifier.scopusauthoridCooper, GJS=7402355946en_HK

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