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Article: Expression of fas antigen (CD95) in peripheral blood lymphocytes and in liver-infiltrating, cytotoxic lymphocytes in patients with hepatocellular carcinoma

TitleExpression of fas antigen (CD95) in peripheral blood lymphocytes and in liver-infiltrating, cytotoxic lymphocytes in patients with hepatocellular carcinoma
Authors
Issue Date2001
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/28741
Citation
Cancer, 2001, v. 92 n. 8, p. 2136-2141 How to Cite?
AbstractBACKGROUND. Fas-expressing cytotoxic T lymphocytes (CTLs) are important antitumor immune effector cells in patients with hepatocellular carcinoma (HCC). The role of transforming growth factor β 1 (TGF-β1) in modulating the expression of Fas by CTLs is not known in HCC. The objectives of this study were to characterize the expression of Fas by CTLs and natural killer (NK) cells among peripheral blood lymphocytes (PBLs) and tumor-infiltrating lymphocytes (TILs) in patients with HCC and to correlate the association, if any, with serum TGF-β1 levels. METHODS. PBLs from 18 patients with HCC and TILs from 5 HCC liver specimens were isolated, and Fas expression was analyzed by three-color flow cytometry. The results were compared with results from normal control volunteers (n = 19 individuals). Serum TGF-β1 levels in patients with HCC were measured by enzyme-linked immunosorbent assay. RESULTS. The median percentage of Fas expression by CD3 positive T cells was significantly higher in patients with HCC compared with normal controls (54.37% vs. 32.03%, respectively; P = 0.0036), and this was attributable solely to Fas expression by CD4 positive PBLs (54.46% vs. 34.90%, respectively; P = 0.0234). In contrast, Fas expression was significantly higher in all the subtypes of TILs (CD3 positive, CD4 positive, CD8 positive, NK cells, and natural T cells) compared with controls (all P values were < 0.001). Tumor size was inversely proportional to the TGF-β1 levels (correlation coefficient [r] = -0.725; P < 0.0001), which were correlated inversely with Fas expression by CD4 positive PBLs (r = -0.516; P = 0.01). CONCLUSIONS. In patients with HCC, TILs exhibit significantly increased expression of Fas compared with PBLs that may enhance their susceptibility to apoptotic mechanisms. Larger tumors were associated with lower serum TGFβ1 levels, and this was correlated with greater Fas expression by CD4 positive PBLs. © 2001 American Cancer Society.
Persistent Identifierhttp://hdl.handle.net/10722/162558
ISSN
2015 Impact Factor: 5.649
2015 SCImago Journal Rankings: 3.188
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYuen, MFen_US
dc.contributor.authorHughes, RDen_US
dc.contributor.authorHeneghan, MAen_US
dc.contributor.authorLangley, PGen_US
dc.contributor.authorNorris, Sen_US
dc.date.accessioned2012-09-05T05:21:08Z-
dc.date.available2012-09-05T05:21:08Z-
dc.date.issued2001en_US
dc.identifier.citationCancer, 2001, v. 92 n. 8, p. 2136-2141en_US
dc.identifier.issn0008-543Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/162558-
dc.description.abstractBACKGROUND. Fas-expressing cytotoxic T lymphocytes (CTLs) are important antitumor immune effector cells in patients with hepatocellular carcinoma (HCC). The role of transforming growth factor β 1 (TGF-β1) in modulating the expression of Fas by CTLs is not known in HCC. The objectives of this study were to characterize the expression of Fas by CTLs and natural killer (NK) cells among peripheral blood lymphocytes (PBLs) and tumor-infiltrating lymphocytes (TILs) in patients with HCC and to correlate the association, if any, with serum TGF-β1 levels. METHODS. PBLs from 18 patients with HCC and TILs from 5 HCC liver specimens were isolated, and Fas expression was analyzed by three-color flow cytometry. The results were compared with results from normal control volunteers (n = 19 individuals). Serum TGF-β1 levels in patients with HCC were measured by enzyme-linked immunosorbent assay. RESULTS. The median percentage of Fas expression by CD3 positive T cells was significantly higher in patients with HCC compared with normal controls (54.37% vs. 32.03%, respectively; P = 0.0036), and this was attributable solely to Fas expression by CD4 positive PBLs (54.46% vs. 34.90%, respectively; P = 0.0234). In contrast, Fas expression was significantly higher in all the subtypes of TILs (CD3 positive, CD4 positive, CD8 positive, NK cells, and natural T cells) compared with controls (all P values were < 0.001). Tumor size was inversely proportional to the TGF-β1 levels (correlation coefficient [r] = -0.725; P < 0.0001), which were correlated inversely with Fas expression by CD4 positive PBLs (r = -0.516; P = 0.01). CONCLUSIONS. In patients with HCC, TILs exhibit significantly increased expression of Fas compared with PBLs that may enhance their susceptibility to apoptotic mechanisms. Larger tumors were associated with lower serum TGFβ1 levels, and this was correlated with greater Fas expression by CD4 positive PBLs. © 2001 American Cancer Society.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/28741en_US
dc.relation.ispartofCanceren_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAntigens, Cd95 - Blood - Metabolismen_US
dc.subject.meshCd4-Positive T-Lymphocytes - Immunologyen_US
dc.subject.meshCd8-Positive T-Lymphocytes - Immunologyen_US
dc.subject.meshCarcinoma, Hepatocellular - Immunologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshFlow Cytometryen_US
dc.subject.meshHumansen_US
dc.subject.meshKiller Cells, Natural - Immunologyen_US
dc.subject.meshLiver Neoplasms - Immunologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshStatistics, Nonparametricen_US
dc.subject.meshT-Lymphocytes, Cytotoxic - Immunologyen_US
dc.subject.meshTransforming Growth Factor Beta - Blooden_US
dc.titleExpression of fas antigen (CD95) in peripheral blood lymphocytes and in liver-infiltrating, cytotoxic lymphocytes in patients with hepatocellular carcinomaen_US
dc.typeArticleen_US
dc.identifier.emailYuen, MF:mfyuen@hkucc.hku.hken_US
dc.identifier.authorityYuen, MF=rp00479en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/1097-0142(20011015)92:8<2136::AID-CNCR1555>3.0.CO;2-Jen_US
dc.identifier.pmid11596030-
dc.identifier.scopuseid_2-s2.0-0035886635en_US
dc.identifier.hkuros131148-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035886635&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume92en_US
dc.identifier.issue8en_US
dc.identifier.spage2136en_US
dc.identifier.epage2141en_US
dc.identifier.isiWOS:000171618100018-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridYuen, MF=7102031955en_US
dc.identifier.scopusauthoridHughes, RD=7404305534en_US
dc.identifier.scopusauthoridHeneghan, MA=7004014958en_US
dc.identifier.scopusauthoridLangley, PG=7102314638en_US
dc.identifier.scopusauthoridNorris, S=7103213449en_US

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