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Article: Validation of a new immunoblot assay for the diagnosis of Helicobacter pylori in the Asian population

TitleValidation of a new immunoblot assay for the diagnosis of Helicobacter pylori in the Asian population
Authors
Issue Date2001
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/APT
Citation
Alimentary Pharmacology And Therapeutics, 2001, v. 15 n. 3, p. 423-428 How to Cite?
AbstractBackground: Performance of commercial serological tests for Helicobacter pylori varies in different populations, largely due to strain heterogeneity and variations in antigenic preparations. Currently available serology tests often show sub-optimal accuracy when used for Asian patients. Aim: This study evaluated a recombinant antigen-based immunoblot for the diagnosis of H. pylori infection in Chinese patients, and compared it with a conventional ELISA test. Methods: Dyspeptic patients referred for diagnostic endoscopy were recruited. The gold standard for H. pylori infection was based on two or more positive results among rapid urease test, histology and 13C-urea breath test. Serological diagnosis of H. pylori infection was conducted by an ELISA test (pylori DTect; Diagnostic Technology) and an immunoblotting against a novel recombinant antigen (C1S; Genelab), which was constructed by immunological screening of the genomic DNA library of H. pylori. Results: A total of 87 patients were evaluated and H. pylori infection was diagnosed in 40 (46%) by the reference tests. The sensitivities of the ELISA and immunoblot were 80% (95% CI: 64-91%) and 90% (95% CI: 76-97%), whilst the specificities were 96% (95% CI: 86-96%) and 87% (95% CI: 74-95%), respectively. The respective likelihood ratios of the two tests were 18.6 and 7.0. Conclusions: Satisfactory performance is obtained by the use of the new recombinant antigen-based immunoblot for diagnosing H. pylori infection in Chinese patients.
Persistent Identifierhttp://hdl.handle.net/10722/162505
ISSN
2015 Impact Factor: 6.32
2015 SCImago Journal Rankings: 2.833
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLeung, WKen_US
dc.contributor.authorChow, TPen_US
dc.contributor.authorNg, EKWen_US
dc.contributor.authorChan, FKLen_US
dc.contributor.authorChung, SCSen_US
dc.contributor.authorSung, JJYen_US
dc.date.accessioned2012-09-05T05:20:35Z-
dc.date.available2012-09-05T05:20:35Z-
dc.date.issued2001en_US
dc.identifier.citationAlimentary Pharmacology And Therapeutics, 2001, v. 15 n. 3, p. 423-428en_US
dc.identifier.issn0269-2813en_US
dc.identifier.urihttp://hdl.handle.net/10722/162505-
dc.description.abstractBackground: Performance of commercial serological tests for Helicobacter pylori varies in different populations, largely due to strain heterogeneity and variations in antigenic preparations. Currently available serology tests often show sub-optimal accuracy when used for Asian patients. Aim: This study evaluated a recombinant antigen-based immunoblot for the diagnosis of H. pylori infection in Chinese patients, and compared it with a conventional ELISA test. Methods: Dyspeptic patients referred for diagnostic endoscopy were recruited. The gold standard for H. pylori infection was based on two or more positive results among rapid urease test, histology and 13C-urea breath test. Serological diagnosis of H. pylori infection was conducted by an ELISA test (pylori DTect; Diagnostic Technology) and an immunoblotting against a novel recombinant antigen (C1S; Genelab), which was constructed by immunological screening of the genomic DNA library of H. pylori. Results: A total of 87 patients were evaluated and H. pylori infection was diagnosed in 40 (46%) by the reference tests. The sensitivities of the ELISA and immunoblot were 80% (95% CI: 64-91%) and 90% (95% CI: 76-97%), whilst the specificities were 96% (95% CI: 86-96%) and 87% (95% CI: 74-95%), respectively. The respective likelihood ratios of the two tests were 18.6 and 7.0. Conclusions: Satisfactory performance is obtained by the use of the new recombinant antigen-based immunoblot for diagnosing H. pylori infection in Chinese patients.en_US
dc.languageengen_US
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/APTen_US
dc.relation.ispartofAlimentary Pharmacology and Therapeuticsen_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAged, 80 And Overen_US
dc.subject.meshAntigens, Bacterial - Analysisen_US
dc.subject.meshAsia - Ethnologyen_US
dc.subject.meshDna, Bacterial - Analysisen_US
dc.subject.meshDiagnosis, Differentialen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshFemaleen_US
dc.subject.meshHelicobacter Infections - Diagnosis - Ethnologyen_US
dc.subject.meshHelicobacter Pylori - Immunologyen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoblotting - Standardsen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshSensitivity And Specificityen_US
dc.titleValidation of a new immunoblot assay for the diagnosis of Helicobacter pylori in the Asian populationen_US
dc.typeArticleen_US
dc.identifier.emailLeung, WK:waikleung@hku.hken_US
dc.identifier.authorityLeung, WK=rp01479en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1046/j.1365-2036.2001.00899.xen_US
dc.identifier.pmid11207519en_US
dc.identifier.scopuseid_2-s2.0-0035111827en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035111827&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume15en_US
dc.identifier.issue3en_US
dc.identifier.spage423en_US
dc.identifier.epage428en_US
dc.identifier.isiWOS:000167004400017-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridLeung, WK=7201504523en_US
dc.identifier.scopusauthoridChow, TP=36798688500en_US
dc.identifier.scopusauthoridNg, EKW=7201647539en_US
dc.identifier.scopusauthoridChan, FKL=7202586434en_US
dc.identifier.scopusauthoridChung, SCS=19642462800en_US
dc.identifier.scopusauthoridSung, JJY=35405352400en_US

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