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Article: The plasmid-stabilizing Ytl2 protein coats DNA in a sequence-independent manner

TitleThe plasmid-stabilizing Ytl2 protein coats DNA in a sequence-independent manner
Authors
Issue Date2001
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yplas
Citation
Plasmid, 2001, v. 46 n. 1, p. 65-70 How to Cite?
AbstractPlasmids carrying the ytl2 gene from the large resident plasmid pSLT of Salmonella typhimurium were stabilized >10 5-fold (compared to control ytl2-free plasmids) in S. typhimurium cells. Purified Ytl2 protein was localized in the cell cytosol and bound to DNA in a sequence-independent manner to form a high-molecular-weight complex, suggesting cooperative binding to the DNA. A mutant ytl2 gene, with a modified C-terminus, did not mediate plasmid stabilization and the mutant Ytl2 protein did not bind cooperatively to DNA. In vivo, while a plasmid carrying the ytl2 gene was stabilized, another plasmid (lacking ytl2) coexisting in the same cell was not. This result suggests that the Ytl2 protein, newly synthesized in a transcription-translation complex, binds preferentially to DNA of the replicon which encodes it and that this binding initiates subsequent cooperative DNA coating by more Ytl2 molecules. Plasmid-encoded newly synthesized Ytl2 protein is thus unavailable to stabilize coresident plasmid DNA, which does not contain the ytl2 gene. © 2001 Academic Press.
Persistent Identifierhttp://hdl.handle.net/10722/162472
ISSN
2015 Impact Factor: 1.732
2015 SCImago Journal Rankings: 0.891
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWong, DKHen_US
dc.contributor.authorMorris, Cen_US
dc.contributor.authorHackett, Jen_US
dc.date.accessioned2012-09-05T05:20:17Z-
dc.date.available2012-09-05T05:20:17Z-
dc.date.issued2001en_US
dc.identifier.citationPlasmid, 2001, v. 46 n. 1, p. 65-70en_US
dc.identifier.issn0147-619Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/162472-
dc.description.abstractPlasmids carrying the ytl2 gene from the large resident plasmid pSLT of Salmonella typhimurium were stabilized >10 5-fold (compared to control ytl2-free plasmids) in S. typhimurium cells. Purified Ytl2 protein was localized in the cell cytosol and bound to DNA in a sequence-independent manner to form a high-molecular-weight complex, suggesting cooperative binding to the DNA. A mutant ytl2 gene, with a modified C-terminus, did not mediate plasmid stabilization and the mutant Ytl2 protein did not bind cooperatively to DNA. In vivo, while a plasmid carrying the ytl2 gene was stabilized, another plasmid (lacking ytl2) coexisting in the same cell was not. This result suggests that the Ytl2 protein, newly synthesized in a transcription-translation complex, binds preferentially to DNA of the replicon which encodes it and that this binding initiates subsequent cooperative DNA coating by more Ytl2 molecules. Plasmid-encoded newly synthesized Ytl2 protein is thus unavailable to stabilize coresident plasmid DNA, which does not contain the ytl2 gene. © 2001 Academic Press.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yplasen_US
dc.relation.ispartofPlasmiden_US
dc.subject.meshAnimalsen_US
dc.subject.meshBacterial Proteins - Genetics - Metabolismen_US
dc.subject.meshDna, Bacterial - Metabolismen_US
dc.subject.meshDna-Binding Proteins - Genetics - Metabolismen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred Balb Cen_US
dc.subject.meshPlasmidsen_US
dc.subject.meshSalmonella Typhimurium - Geneticsen_US
dc.titleThe plasmid-stabilizing Ytl2 protein coats DNA in a sequence-independent manneren_US
dc.typeArticleen_US
dc.identifier.emailWong, DKH:danywong@hku.hken_US
dc.identifier.authorityWong, DKH=rp00492en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1006/plas.2001.1520en_US
dc.identifier.pmid11535038-
dc.identifier.scopuseid_2-s2.0-0034853276en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034853276&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume46en_US
dc.identifier.issue1en_US
dc.identifier.spage65en_US
dc.identifier.epage70en_US
dc.identifier.isiWOS:000170966400009-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWong, DKH=7401535819en_US
dc.identifier.scopusauthoridMorris, C=7401472993en_US
dc.identifier.scopusauthoridHackett, J=24302040000en_US

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