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Article: Identification of a second MutL DNA mismatch repair complex (hPMS1 and hMLH1) in human epithelial cells

TitleIdentification of a second MutL DNA mismatch repair complex (hPMS1 and hMLH1) in human epithelial cells
Authors
Issue Date2000
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
Citation
Journal Of Biological Chemistry, 2000, v. 275 n. 21, p. 15728-15732 How to Cite?
AbstractDeficiencies of MutL DNA mismatch repair-complex proteins (hMLH1, hPMS2, and hPMS1) typically result in microsatellite instability in human cancers. We examined the association patterns of MutL proteins in human epithelial cancer cell lines with (HCT-116, N87, SNU-1, and SNU-638) and without microsatellite instability (HeLa, AGS, KATO-III, and SNU-16). The analysis of hMLH1, hPMS2, and hPMS1 was performed using Northern blot, Western blot, and co-immunoprecipitation studies. Our data provide evidence that MutL proteins form two different complexes, MutL-α (hPMS2 and hMLH1) and MutL-β (hPMS1 and hMLH1). Gastric and colorectal cancer cells lines with microsatellite instability lacked detectable hMLH1. Decreased levels of hMLH1 protein were associated with markedly reduced levels of hPMS2 and hPMS1 proteins, but the RNA levels of hPMS1 and hPMS2 were normal. In this study, we describe the association of hPMS1 with hMLH1 as a heterodimer, in human cells. Furthermore, normal levels of hMLH1 protein appear to be important in maintaining normal levels of hPMS1 and hPMS2 proteins.
Persistent Identifierhttp://hdl.handle.net/10722/162455
ISSN
2015 Impact Factor: 4.258
2015 SCImago Journal Rankings: 3.151
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLeung, WKen_US
dc.contributor.authorKim, JJen_US
dc.contributor.authorWu, Len_US
dc.contributor.authorSepulveda, JLen_US
dc.contributor.authorSepulveda, ARen_US
dc.date.accessioned2012-09-05T05:20:05Z-
dc.date.available2012-09-05T05:20:05Z-
dc.date.issued2000en_US
dc.identifier.citationJournal Of Biological Chemistry, 2000, v. 275 n. 21, p. 15728-15732en_US
dc.identifier.issn0021-9258en_US
dc.identifier.urihttp://hdl.handle.net/10722/162455-
dc.description.abstractDeficiencies of MutL DNA mismatch repair-complex proteins (hMLH1, hPMS2, and hPMS1) typically result in microsatellite instability in human cancers. We examined the association patterns of MutL proteins in human epithelial cancer cell lines with (HCT-116, N87, SNU-1, and SNU-638) and without microsatellite instability (HeLa, AGS, KATO-III, and SNU-16). The analysis of hMLH1, hPMS2, and hPMS1 was performed using Northern blot, Western blot, and co-immunoprecipitation studies. Our data provide evidence that MutL proteins form two different complexes, MutL-α (hPMS2 and hMLH1) and MutL-β (hPMS1 and hMLH1). Gastric and colorectal cancer cells lines with microsatellite instability lacked detectable hMLH1. Decreased levels of hMLH1 protein were associated with markedly reduced levels of hPMS2 and hPMS1 proteins, but the RNA levels of hPMS1 and hPMS2 were normal. In this study, we describe the association of hPMS1 with hMLH1 as a heterodimer, in human cells. Furthermore, normal levels of hMLH1 protein appear to be important in maintaining normal levels of hPMS1 and hPMS2 proteins.en_US
dc.languageengen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/en_US
dc.relation.ispartofJournal of Biological Chemistryen_US
dc.subject.meshAdaptor Proteins, Signal Transducingen_US
dc.subject.meshAdenosine Triphosphatasesen_US
dc.subject.meshBase Pair Mismatch - Geneticsen_US
dc.subject.meshCarrier Proteins - Genetics - Metabolismen_US
dc.subject.meshDna Repair - Geneticsen_US
dc.subject.meshDna Repair Enzymesen_US
dc.subject.meshDna-Binding Proteinsen_US
dc.subject.meshDimerizationen_US
dc.subject.meshGene Expression Regulation, Neoplasticen_US
dc.subject.meshHumansen_US
dc.subject.meshMicrosatellite Repeats - Geneticsen_US
dc.subject.meshNeoplasm Proteins - Genetics - Metabolismen_US
dc.subject.meshNuclear Proteinsen_US
dc.subject.meshPrecipitin Testsen_US
dc.subject.meshProteins - Genetics - Metabolismen_US
dc.subject.meshRna, Messenger - Metabolismen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.titleIdentification of a second MutL DNA mismatch repair complex (hPMS1 and hMLH1) in human epithelial cellsen_US
dc.typeArticleen_US
dc.identifier.emailLeung, WK:waikleung@hku.hken_US
dc.identifier.authorityLeung, WK=rp01479en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1074/jbc.M908768199en_US
dc.identifier.pmid10748105-
dc.identifier.scopuseid_2-s2.0-0034717283en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034717283&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume275en_US
dc.identifier.issue21en_US
dc.identifier.spage15728en_US
dc.identifier.epage15732en_US
dc.identifier.isiWOS:000087291400019-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLeung, WK=7201504523en_US
dc.identifier.scopusauthoridKim, JJ=36067967700en_US
dc.identifier.scopusauthoridWu, L=7404904649en_US
dc.identifier.scopusauthoridSepulveda, JL=7005033581en_US
dc.identifier.scopusauthoridSepulveda, AR=35500186600en_US

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