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Article: Salmonella enterica serovar Typhi uses type IVB pili to enter human intestinal epithelial cells

TitleSalmonella enterica serovar Typhi uses type IVB pili to enter human intestinal epithelial cells
Authors
Issue Date2000
Citation
Infection And Immunity, 2000, v. 68 n. 6, p. 3067-3073 How to Cite?
AbstractDNA sequencing upstream of the Salmonella enterica serovar Typhi pilV and rci genes previously identified in the ca. 118-kb major pathogenicity island (X.-L. Zhang, C. Morris, and J. Hackett, Gene 202:139-146, 1997) identified a further 10 pil genes apparently forming a pil operon. The product of the pilS gene, prePilS protein (a putative type IVB structural prepilin) was purified, and an anti-prePilS antiserum was raised in mice. Mutants of serovar Typhi either lacking the whole pil operon or with an insertion mutation in the pilS gene were constructed, as was a strain in which the pilN to pilV genes were driven by the tac promoter. The pil + strains synthesized type IVB pili, as judged by (i) visualization in the electron microscope of thin pili in culture supernatants of one such strain and (ii) the presence of PilS protein (smaller than the prePilS protein by removal of the leader peptide) on immunoblotting of material pelleted by high-speed centrifugation of either the culture supernatant or sonicates of pil + strains. Control pil mutants did not express the PilS protein. A pilS mutant of serovar Typhi entered human intestinal INT407 cells in culture to levels only 5 to 25% of those of the wild-type strain, and serovar Typhi entry was strongly inhibited by soluble prePilS protein (50% inhibition of entry at 1.4 μM prePilS).
Persistent Identifierhttp://hdl.handle.net/10722/162427
ISSN
2015 Impact Factor: 3.603
2015 SCImago Journal Rankings: 2.342
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhang, XLen_US
dc.contributor.authorTsui, ISMen_US
dc.contributor.authorYip, CMCen_US
dc.contributor.authorFung, AWYen_US
dc.contributor.authorWong, DKHen_US
dc.contributor.authorDai, Xen_US
dc.contributor.authorYang, Yen_US
dc.contributor.authorHackett, Jen_US
dc.contributor.authorMorris, Cen_US
dc.date.accessioned2012-09-05T05:19:49Z-
dc.date.available2012-09-05T05:19:49Z-
dc.date.issued2000en_US
dc.identifier.citationInfection And Immunity, 2000, v. 68 n. 6, p. 3067-3073en_US
dc.identifier.issn0019-9567en_US
dc.identifier.urihttp://hdl.handle.net/10722/162427-
dc.description.abstractDNA sequencing upstream of the Salmonella enterica serovar Typhi pilV and rci genes previously identified in the ca. 118-kb major pathogenicity island (X.-L. Zhang, C. Morris, and J. Hackett, Gene 202:139-146, 1997) identified a further 10 pil genes apparently forming a pil operon. The product of the pilS gene, prePilS protein (a putative type IVB structural prepilin) was purified, and an anti-prePilS antiserum was raised in mice. Mutants of serovar Typhi either lacking the whole pil operon or with an insertion mutation in the pilS gene were constructed, as was a strain in which the pilN to pilV genes were driven by the tac promoter. The pil + strains synthesized type IVB pili, as judged by (i) visualization in the electron microscope of thin pili in culture supernatants of one such strain and (ii) the presence of PilS protein (smaller than the prePilS protein by removal of the leader peptide) on immunoblotting of material pelleted by high-speed centrifugation of either the culture supernatant or sonicates of pil + strains. Control pil mutants did not express the PilS protein. A pilS mutant of serovar Typhi entered human intestinal INT407 cells in culture to levels only 5 to 25% of those of the wild-type strain, and serovar Typhi entry was strongly inhibited by soluble prePilS protein (50% inhibition of entry at 1.4 μM prePilS).en_US
dc.languageengen_US
dc.relation.ispartofInfection and Immunityen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshBacterial Adhesionen_US
dc.subject.meshBacterial Proteins - Geneticsen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshEpithelial Cells - Microbiologyen_US
dc.subject.meshFimbriae Proteinsen_US
dc.subject.meshFimbriae, Bacterial - Genetics - Ultrastructureen_US
dc.subject.meshHumansen_US
dc.subject.meshIntestinal Mucosa - Microbiologyen_US
dc.subject.meshMembrane Proteins - Geneticsen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshOperonen_US
dc.subject.meshProtein Precursors - Geneticsen_US
dc.subject.meshSalmonella Typhi - Genetics - Pathogenicityen_US
dc.subject.meshSequence Homology, Amino Aciden_US
dc.subject.meshSerotypingen_US
dc.subject.meshTranscription Factors - Geneticsen_US
dc.subject.meshVirulence - Geneticsen_US
dc.titleSalmonella enterica serovar Typhi uses type IVB pili to enter human intestinal epithelial cellsen_US
dc.typeArticleen_US
dc.identifier.emailWong, DKH:danywong@hku.hken_US
dc.identifier.authorityWong, DKH=rp00492en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1128/IAI.68.6.3067-3073.2000en_US
dc.identifier.pmid10816445-
dc.identifier.scopuseid_2-s2.0-0034115689en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034115689&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume68en_US
dc.identifier.issue6en_US
dc.identifier.spage3067en_US
dc.identifier.epage3073en_US
dc.identifier.isiWOS:000087167900001-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridZhang, XL=35555551600en_US
dc.identifier.scopusauthoridTsui, ISM=6602387676en_US
dc.identifier.scopusauthoridYip, CMC=7101665509en_US
dc.identifier.scopusauthoridFung, AWY=7101926771en_US
dc.identifier.scopusauthoridWong, DKH=7401535819en_US
dc.identifier.scopusauthoridDai, X=55237284600en_US
dc.identifier.scopusauthoridYang, Y=7409385318en_US
dc.identifier.scopusauthoridHackett, J=24302040000en_US
dc.identifier.scopusauthoridMorris, C=7401472993en_US
dc.identifier.citeulike5183691-

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