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Article: Overexpression of protein kinase C-β1 isoenzyme suppresses indomethacin-induced apoptosis in gastric epithelial cells

TitleOverexpression of protein kinase C-β1 isoenzyme suppresses indomethacin-induced apoptosis in gastric epithelial cells
Authors
Issue Date2000
PublisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/gastro
Citation
Gastroenterology, 2000, v. 118 n. 3, p. 507-514 How to Cite?
AbstractBackground and Aims: We have previously reported that nonsteroidal anti- inflammatory drugs (NSAIDs) could induce apoptosis of gastric epithelial cells both in vivo and in vitro. This study investigated the role of protein kinase C (PKC) isoforms in the regulation of NSAID-induced apoptosis. Methods: Protein levels of 12 PKC isoforms in AGS cells, in the presence or absence of indomethacin, were determined by western blot. The effect of PKC- β1 overexpression by transfection with its complementary DNA (cDNA) on indomethacin-induced apoptosis and apoptosis-related genes, including p53, p21(waf1/cip1), and c-myc, was further investigated. Results: Treatment with indomethacin decreased the abundance of PKC-β1 and increased that of PKCβ2, η, and ε, but did not alter the expression of PKC α, γ, ζ, δ, ι, and μ. Overexpression of PKC-β1 attenuated the apoptotic response of AGS cells to indomethacin, associated with overexpression of p21(waf1/cip1) in both messenger RNA and protein levels. Inhibition of PKC-β1-mediated overexpression of p21(waf1/cip1) by its antisense cDNA partially reduced the antiapoptotic effect of PKC-β1. Conclusions: Indomethacin-induced apoptosis in gastric cancer cells is partly mediated by differential regulation of PKC isoform expression. Enhanced expression of exogenous PKC-β1 protects against indomethacin-induced apoptosis through up-regulation of p21(waf1/cip1).
Persistent Identifierhttp://hdl.handle.net/10722/162415
ISSN
2015 Impact Factor: 18.187
2015 SCImago Journal Rankings: 7.170
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhu, GHen_US
dc.contributor.authorWong, BCYen_US
dc.contributor.authorSlosberg, EDen_US
dc.contributor.authorEggo, MCen_US
dc.contributor.authorChing, CKen_US
dc.contributor.authorYuen, STen_US
dc.contributor.authorLai, KCen_US
dc.contributor.authorSoh, JWen_US
dc.contributor.authorWeinstein, IBen_US
dc.contributor.authorLam, SKen_US
dc.date.accessioned2012-09-05T05:19:44Z-
dc.date.available2012-09-05T05:19:44Z-
dc.date.issued2000en_US
dc.identifier.citationGastroenterology, 2000, v. 118 n. 3, p. 507-514en_US
dc.identifier.issn0016-5085en_US
dc.identifier.urihttp://hdl.handle.net/10722/162415-
dc.description.abstractBackground and Aims: We have previously reported that nonsteroidal anti- inflammatory drugs (NSAIDs) could induce apoptosis of gastric epithelial cells both in vivo and in vitro. This study investigated the role of protein kinase C (PKC) isoforms in the regulation of NSAID-induced apoptosis. Methods: Protein levels of 12 PKC isoforms in AGS cells, in the presence or absence of indomethacin, were determined by western blot. The effect of PKC- β1 overexpression by transfection with its complementary DNA (cDNA) on indomethacin-induced apoptosis and apoptosis-related genes, including p53, p21(waf1/cip1), and c-myc, was further investigated. Results: Treatment with indomethacin decreased the abundance of PKC-β1 and increased that of PKCβ2, η, and ε, but did not alter the expression of PKC α, γ, ζ, δ, ι, and μ. Overexpression of PKC-β1 attenuated the apoptotic response of AGS cells to indomethacin, associated with overexpression of p21(waf1/cip1) in both messenger RNA and protein levels. Inhibition of PKC-β1-mediated overexpression of p21(waf1/cip1) by its antisense cDNA partially reduced the antiapoptotic effect of PKC-β1. Conclusions: Indomethacin-induced apoptosis in gastric cancer cells is partly mediated by differential regulation of PKC isoform expression. Enhanced expression of exogenous PKC-β1 protects against indomethacin-induced apoptosis through up-regulation of p21(waf1/cip1).en_US
dc.languageengen_US
dc.publisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/gastroen_US
dc.relation.ispartofGastroenterologyen_US
dc.subject.meshAnti-Inflammatory Agents, Non-Steroidal - Pharmacologyen_US
dc.subject.meshAntisense Elements (Genetics) - Pharmacologyen_US
dc.subject.meshApoptosis - Drug Effectsen_US
dc.subject.meshCyclin-Dependent Kinase Inhibitor P21en_US
dc.subject.meshCyclins - Genetics - Metabolismen_US
dc.subject.meshDna, Complementary - Pharmacologyen_US
dc.subject.meshGastric Mucosa - Enzymology - Pathology - Physiopathologyen_US
dc.subject.meshIndomethacin - Pharmacologyen_US
dc.subject.meshIsoenzymes - Metabolismen_US
dc.subject.meshProtein Kinase C - Metabolismen_US
dc.subject.meshProto-Oncogene Proteins C-Myc - Metabolismen_US
dc.subject.meshTransfectionen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.subject.meshTumor Suppressor Protein P53 - Metabolismen_US
dc.titleOverexpression of protein kinase C-β1 isoenzyme suppresses indomethacin-induced apoptosis in gastric epithelial cellsen_US
dc.typeArticleen_US
dc.identifier.emailWong, BCY:bcywong@hku.hken_US
dc.identifier.authorityWong, BCY=rp00429en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0016-5085(00)70256-3-
dc.identifier.pmid10702201-
dc.identifier.scopuseid_2-s2.0-0034056322en_US
dc.identifier.hkuros50516-
dc.identifier.volume118en_US
dc.identifier.issue3en_US
dc.identifier.spage507en_US
dc.identifier.epage514en_US
dc.identifier.isiWOS:000085710500011-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridZhu, GH=7402633170en_US
dc.identifier.scopusauthoridWong, BCY=7402023340en_US
dc.identifier.scopusauthoridSlosberg, ED=6604090681en_US
dc.identifier.scopusauthoridEggo, MC=7006000548en_US
dc.identifier.scopusauthoridChing, CK=7102130825en_US
dc.identifier.scopusauthoridYuen, ST=7103160927en_US
dc.identifier.scopusauthoridLai, KC=7402135595en_US
dc.identifier.scopusauthoridSoh, JW=7006815014en_US
dc.identifier.scopusauthoridWeinstein, IB=36048534800en_US
dc.identifier.scopusauthoridLam, SK=7402279473en_US

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