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Article: Prolonged storage of frozen unbuffered rapid urease test

TitleProlonged storage of frozen unbuffered rapid urease test
Authors
Issue Date2000
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.jcge.com
Citation
Journal Of Clinical Gastroenterology, 2000, v. 31 n. 2, p. 142-143 How to Cite?
AbstractUnbuffered rapid urease test (RUT) is an accurate, rapid, and inexpensive method of detecting Helicobacter pylori. However, it is generally recommended to prepare the reagent daily. This prospective study was undertaken to evaluate the shelf-life of our unbuffered RUT at -20°C. Two sets of antral biopsies (X2) and body biopsies (X1) were taken from all patients. They were subjected to histologic examination and RUT stored at -20°C. We studied 303 patients. One hundred seventy-nine (59%) were infected as defined by histologic examination. The reagent was classified according to the storage time (group I, 0-30 days [n = 109]; group II, 31-60 days [n = 63]; group III, 61-90 days [n = 80]; and group IV, τ91 days [n = 51]). There was an excellent agreement between the histologic examination and the RUT of the four groups (kappa coefficient: group I, 0.88; group II, 0.80; group III, 0.87; group IV, 0.88). The sensitivity of our RUT in groups I, II, III, and IV were 92%, 92%, 91%, and 89%, respectively. The corresponding specificity were 97%, 88%, 97%, and 100%. Our RUT remains highly sensitive and specific when it is stored at -20°C for 4 months.
Persistent Identifierhttp://hdl.handle.net/10722/162387
ISSN
2015 Impact Factor: 3.163
2015 SCImago Journal Rankings: 1.274
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorNg, FHen_US
dc.contributor.authorWong, SYen_US
dc.contributor.authorNg, WFen_US
dc.contributor.authorWong, BCYen_US
dc.date.accessioned2012-09-05T05:19:30Z-
dc.date.available2012-09-05T05:19:30Z-
dc.date.issued2000en_US
dc.identifier.citationJournal Of Clinical Gastroenterology, 2000, v. 31 n. 2, p. 142-143en_US
dc.identifier.issn0192-0790en_US
dc.identifier.urihttp://hdl.handle.net/10722/162387-
dc.description.abstractUnbuffered rapid urease test (RUT) is an accurate, rapid, and inexpensive method of detecting Helicobacter pylori. However, it is generally recommended to prepare the reagent daily. This prospective study was undertaken to evaluate the shelf-life of our unbuffered RUT at -20°C. Two sets of antral biopsies (X2) and body biopsies (X1) were taken from all patients. They were subjected to histologic examination and RUT stored at -20°C. We studied 303 patients. One hundred seventy-nine (59%) were infected as defined by histologic examination. The reagent was classified according to the storage time (group I, 0-30 days [n = 109]; group II, 31-60 days [n = 63]; group III, 61-90 days [n = 80]; and group IV, τ91 days [n = 51]). There was an excellent agreement between the histologic examination and the RUT of the four groups (kappa coefficient: group I, 0.88; group II, 0.80; group III, 0.87; group IV, 0.88). The sensitivity of our RUT in groups I, II, III, and IV were 92%, 92%, 91%, and 89%, respectively. The corresponding specificity were 97%, 88%, 97%, and 100%. Our RUT remains highly sensitive and specific when it is stored at -20°C for 4 months.en_US
dc.languageengen_US
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.jcge.comen_US
dc.relation.ispartofJournal of Clinical Gastroenterologyen_US
dc.rightsJournal of Clinical Gastroenterology. Copyright © Lippincott Williams & Wilkins.-
dc.subject.meshBiopsyen_US
dc.subject.meshFemaleen_US
dc.subject.meshFreezingen_US
dc.subject.meshGastroscopyen_US
dc.subject.meshHelicobacter Infections - Diagnosis - Pathologyen_US
dc.subject.meshHelicobacter Pylori - Isolation & Purificationen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshProspective Studiesen_US
dc.subject.meshReagent Kits, Diagnosticen_US
dc.subject.meshSensitivity And Specificityen_US
dc.subject.meshStomach - Microbiology - Pathologyen_US
dc.subject.meshTime Factorsen_US
dc.subject.meshUrease - Analysisen_US
dc.titleProlonged storage of frozen unbuffered rapid urease testen_US
dc.typeArticleen_US
dc.identifier.emailWong, BCY:bcywong@hku.hken_US
dc.identifier.authorityWong, BCY=rp00429en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1097/00004836-200009000-00010en_US
dc.identifier.pmid10993430en_US
dc.identifier.scopuseid_2-s2.0-0033850127en_US
dc.identifier.hkuros61789-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033850127&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume31en_US
dc.identifier.issue2en_US
dc.identifier.spage142en_US
dc.identifier.epage143en_US
dc.identifier.isiWOS:000089056100010-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridNg, FH=16936078000en_US
dc.identifier.scopusauthoridWong, SY=7404590845en_US
dc.identifier.scopusauthoridNg, WF=25940213100en_US
dc.identifier.scopusauthoridWong, BCY=7402023340en_US

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