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Article: Expression of translationally controlled tumour protein is regulated by calcium at both the transcriptional and post-transcriptional level

TitleExpression of translationally controlled tumour protein is regulated by calcium at both the transcriptional and post-transcriptional level
Authors
Issue Date1999
PublisherPortland Press Ltd. The Journal's web site is located at http://www.biochemj.org
Citation
Biochemical Journal, 1999, v. 342 n. 3, p. 683-689 How to Cite?
AbstractWe have investigated how the programme of protein synthesis is altered in response to a loss of calcium homoeostasis in Cos-7 cells using a differential proteome mapping approach. Exposure of the cells to the calcium ionophore A23187 or thapsigargin, or alternatively, expression of a viral glycoprotein reported to deplete intracellular calcium stores, resulted in the up-regulated expression of a characteristic set of proteins. One of these is the translationally controlled tumour protein (TCTP), a cytoplasmic protein whose expression has not previously been linked to calcium perturbation. Quantitative Northern blot assay demonstrated that steady-state mRNA abundance of TCTP was also increased under these conditions. Clamping the cytosolic calcium concentration by the introduction of bis-(o-aminophenoxy)-ethane-N,N,N',N'-tetra-acetic acid (BAPTA) into cells did not affect the increase in steady-state levels of TCTP mRNA observed in response to ionophore. Therefore depletion of endoplasmic reticulum (ER) calcium, but not elevation of the cytosolic calcium concentration, was responsible for increased transcription of the TCTP gene. However, the presence of BAPTA significantly attenuated the ionophore-mediated increase in levels of the protein. Moreover, the level of TCTP in ionophore-treated cells increased in advance of a detectable increase in the corresponding mRNA abundance. These results indicate that expression of TCTP is regulated at two distinct levels in response to the concentration of calcium in different cellular compartments. Whereas depletion of the ER store causes an increase in TCTP mRNA abundance, increased cytosolic calcium concentrations regulate gene expression at the post-transcriptional level.
Persistent Identifierhttp://hdl.handle.net/10722/162363
ISSN
2015 Impact Factor: 3.562
2015 SCImago Journal Rankings: 2.582
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorXu, Aen_US
dc.contributor.authorBellamy, ARen_US
dc.contributor.authorTaylor, JAen_US
dc.date.accessioned2012-09-05T05:19:17Z-
dc.date.available2012-09-05T05:19:17Z-
dc.date.issued1999en_US
dc.identifier.citationBiochemical Journal, 1999, v. 342 n. 3, p. 683-689en_US
dc.identifier.issn0264-6021en_US
dc.identifier.urihttp://hdl.handle.net/10722/162363-
dc.description.abstractWe have investigated how the programme of protein synthesis is altered in response to a loss of calcium homoeostasis in Cos-7 cells using a differential proteome mapping approach. Exposure of the cells to the calcium ionophore A23187 or thapsigargin, or alternatively, expression of a viral glycoprotein reported to deplete intracellular calcium stores, resulted in the up-regulated expression of a characteristic set of proteins. One of these is the translationally controlled tumour protein (TCTP), a cytoplasmic protein whose expression has not previously been linked to calcium perturbation. Quantitative Northern blot assay demonstrated that steady-state mRNA abundance of TCTP was also increased under these conditions. Clamping the cytosolic calcium concentration by the introduction of bis-(o-aminophenoxy)-ethane-N,N,N',N'-tetra-acetic acid (BAPTA) into cells did not affect the increase in steady-state levels of TCTP mRNA observed in response to ionophore. Therefore depletion of endoplasmic reticulum (ER) calcium, but not elevation of the cytosolic calcium concentration, was responsible for increased transcription of the TCTP gene. However, the presence of BAPTA significantly attenuated the ionophore-mediated increase in levels of the protein. Moreover, the level of TCTP in ionophore-treated cells increased in advance of a detectable increase in the corresponding mRNA abundance. These results indicate that expression of TCTP is regulated at two distinct levels in response to the concentration of calcium in different cellular compartments. Whereas depletion of the ER store causes an increase in TCTP mRNA abundance, increased cytosolic calcium concentrations regulate gene expression at the post-transcriptional level.en_US
dc.languageengen_US
dc.publisherPortland Press Ltd. The Journal's web site is located at http://www.biochemj.orgen_US
dc.relation.ispartofBiochemical Journalen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntiviral Agentsen_US
dc.subject.meshCos Cellsen_US
dc.subject.meshCalcimycin - Pharmacologyen_US
dc.subject.meshCalcium - Physiologyen_US
dc.subject.meshCalcium-Binding Proteins - Biosynthesis - Geneticsen_US
dc.subject.meshCytosol - Metabolismen_US
dc.subject.meshDna-Directed Rna Polymerasesen_US
dc.subject.meshDithiothreitol - Pharmacologyen_US
dc.subject.meshEndoplasmic Reticulum - Metabolismen_US
dc.subject.meshNeoplasm Proteins - Biosynthesis - Geneticsen_US
dc.subject.meshProtein Biosynthesisen_US
dc.subject.meshProtein Processing, Post-Translationalen_US
dc.subject.meshRotavirusen_US
dc.subject.meshStress, Physiological - Metabolismen_US
dc.subject.meshTranscription, Geneticen_US
dc.subject.meshTransfectionen_US
dc.subject.meshTumor Markers, Biologicalen_US
dc.subject.meshTunicamycin - Pharmacologyen_US
dc.subject.meshUp-Regulationen_US
dc.subject.meshViral Nonstructural Proteins - Biosynthesis - Genetics - Physiologyen_US
dc.titleExpression of translationally controlled tumour protein is regulated by calcium at both the transcriptional and post-transcriptional levelen_US
dc.typeArticleen_US
dc.identifier.emailXu, A:amxu@hkucc.hku.hken_US
dc.identifier.authorityXu, A=rp00485en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1042/0264-6021:3420683en_US
dc.identifier.pmid10477280-
dc.identifier.scopuseid_2-s2.0-0033568290en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033568290&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume342en_US
dc.identifier.issue3en_US
dc.identifier.spage683en_US
dc.identifier.epage689en_US
dc.identifier.isiWOS:000082848300024-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridXu, A=7202655409en_US
dc.identifier.scopusauthoridBellamy, AR=7004515654en_US
dc.identifier.scopusauthoridTaylor, JA=24781484000en_US

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