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Article: Gene expression and synthesis of natriuretic peptides by cultured human glomerular cells

TitleGene expression and synthesis of natriuretic peptides by cultured human glomerular cells
Authors
Issue Date1999
PublisherLippincott Williams & Wilkins, Ltd. The Journal's web site is located at http://www.jhypertension.com/
Citation
Journal Of Hypertension, 1999, v. 17 n. 4, p. 575-583 How to Cite?
AbstractBackground. Atrial natriuretic peptide, brain natriuretic peptide and C-type natriuretic peptide belong to a family of hormones that have natriuretic and vasodepressor activity and may play a pathophysiologic role in hypertension, heart failure and renal failure. Whereas immunoreactive human forms of these three natriuretic peptides are found in renal tubules, it is not clear whether they are derived from the systemic circulation or from local production. Objective. To examine the gene expression of natriuretic peptides in cultured human glomerular cells. Materials and methods. We sought to determine the presence of messenger RNA encoding for these natriuretic peptides using polymerase chain reaction following reverse transcription. The polymerase chain reaction products were confirmed by direct sequencing. Atrial natriuretic peptide, brain natriuretic peptide and C-type natriuretic peptide in cell-culture supernatants were measured by radioimmunoassays (with detection limits of 2.1, 2.1 and 0.21 pmol/l, respectively). Results. Atrial natriuretic peptide messenger RNA was not found in mesangial or glomerular epithelial cells (despite stimulation with tumor necrosis factor-α) except when the cells were cultured with a high concentration of fetal bovine serum (> 10%). Similarly, this peptide was not detected in supernatant unless the cells were cultured with fetal bovine serum at concentrations of > 10%. Brain natriuretic peptide messenger RNA was readily detected in cultured mesangial and glomerular epithelial cells with a lower concentration in the former. Brain natriuretic peptide was not found in the supernatant of resting mesangial cells but became detectable when incubated with tumor necrosis factor-α or fetal bovine serum. C-type natriuretic peptide messenger RNA was detected in mesangial and glomerular epithelial cells with a higher concentration in the latter. C-type natriuretic peptide was detected in the supernatant of resting glomerular epithelial cells and levels rose when incubated with increasing concentrations of tumor necrosis factor-α or fetal bovine serum. However, C-type natriuretic peptide was not detected in the supernatant of resting mesangial cells and remained undetectable following incubation with tumor necrosis factor-α or fetal bovine serum. Conclusion. Our results suggest differences in the synthesis of natriuretic peptides between glomerular mesangial and epithelial cells.
Persistent Identifierhttp://hdl.handle.net/10722/162341
ISSN
2015 Impact Factor: 5.062
2015 SCImago Journal Rankings: 2.193
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLai, KNen_US
dc.contributor.authorLeung, JCKen_US
dc.contributor.authorYandle, TGen_US
dc.contributor.authorFisher, Sen_US
dc.contributor.authorNicholls, MGen_US
dc.date.accessioned2012-09-05T05:19:08Z-
dc.date.available2012-09-05T05:19:08Z-
dc.date.issued1999en_US
dc.identifier.citationJournal Of Hypertension, 1999, v. 17 n. 4, p. 575-583en_US
dc.identifier.issn0263-6352en_US
dc.identifier.urihttp://hdl.handle.net/10722/162341-
dc.description.abstractBackground. Atrial natriuretic peptide, brain natriuretic peptide and C-type natriuretic peptide belong to a family of hormones that have natriuretic and vasodepressor activity and may play a pathophysiologic role in hypertension, heart failure and renal failure. Whereas immunoreactive human forms of these three natriuretic peptides are found in renal tubules, it is not clear whether they are derived from the systemic circulation or from local production. Objective. To examine the gene expression of natriuretic peptides in cultured human glomerular cells. Materials and methods. We sought to determine the presence of messenger RNA encoding for these natriuretic peptides using polymerase chain reaction following reverse transcription. The polymerase chain reaction products were confirmed by direct sequencing. Atrial natriuretic peptide, brain natriuretic peptide and C-type natriuretic peptide in cell-culture supernatants were measured by radioimmunoassays (with detection limits of 2.1, 2.1 and 0.21 pmol/l, respectively). Results. Atrial natriuretic peptide messenger RNA was not found in mesangial or glomerular epithelial cells (despite stimulation with tumor necrosis factor-α) except when the cells were cultured with a high concentration of fetal bovine serum (> 10%). Similarly, this peptide was not detected in supernatant unless the cells were cultured with fetal bovine serum at concentrations of > 10%. Brain natriuretic peptide messenger RNA was readily detected in cultured mesangial and glomerular epithelial cells with a lower concentration in the former. Brain natriuretic peptide was not found in the supernatant of resting mesangial cells but became detectable when incubated with tumor necrosis factor-α or fetal bovine serum. C-type natriuretic peptide messenger RNA was detected in mesangial and glomerular epithelial cells with a higher concentration in the latter. C-type natriuretic peptide was detected in the supernatant of resting glomerular epithelial cells and levels rose when incubated with increasing concentrations of tumor necrosis factor-α or fetal bovine serum. However, C-type natriuretic peptide was not detected in the supernatant of resting mesangial cells and remained undetectable following incubation with tumor necrosis factor-α or fetal bovine serum. Conclusion. Our results suggest differences in the synthesis of natriuretic peptides between glomerular mesangial and epithelial cells.en_US
dc.languageengen_US
dc.publisherLippincott Williams & Wilkins, Ltd. The Journal's web site is located at http://www.jhypertension.com/en_US
dc.relation.ispartofJournal of Hypertensionen_US
dc.rightsJournal of Hypertension. Copyright © Lippincott Williams & Wilkins, Ltd.-
dc.subject.meshAtrial Natriuretic Factor - Biosynthesis - Geneticsen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshDactinomycin - Pharmacologyen_US
dc.subject.meshEpithelial Cells - Metabolism - Physiologyen_US
dc.subject.meshGene Expressionen_US
dc.subject.meshGlomerular Mesangium - Drug Effects - Metabolism - Physiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshNatriuretic Peptide, Brain - Biosynthesis - Geneticsen_US
dc.subject.meshNatriuretic Peptide, C-Type - Biosynthesis - Geneticsen_US
dc.subject.meshProtein Synthesis Inhibitors - Pharmacologyen_US
dc.subject.meshPuromycin - Pharmacologyen_US
dc.subject.meshRna, Messenger - Analysis - Metabolismen_US
dc.titleGene expression and synthesis of natriuretic peptides by cultured human glomerular cellsen_US
dc.typeArticleen_US
dc.identifier.emailLeung, JCK:jckleung@hku.hken_US
dc.identifier.authorityLeung, JCK=rp00448en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1097/00004872-199917040-00017en_US
dc.identifier.pmid10404961-
dc.identifier.scopuseid_2-s2.0-0033049953en_US
dc.identifier.hkuros41550-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033049953&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume17en_US
dc.identifier.issue4en_US
dc.identifier.spage575en_US
dc.identifier.epage583en_US
dc.identifier.isiWOS:000080341900017-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridKar Neng, L=6504632548en_US
dc.identifier.scopusauthoridLeung, JCK=7202180349en_US
dc.identifier.scopusauthoridYandle, TG=7006761477en_US
dc.identifier.scopusauthoridFisher, S=15821832200en_US
dc.identifier.scopusauthoridNicholls, MG=7202358024en_US
dc.customcontrol.immutablejt 130530-

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