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Article: Growth factors in continuous ambulatory peritoneal dialysis effluent: their relation with peritoneal transport of small solutes

TitleGrowth factors in continuous ambulatory peritoneal dialysis effluent: their relation with peritoneal transport of small solutes
Authors
KeywordsContinuous ambulatory peritoneal dialysis
Macrophages
Mesothelial cells
Peritoneal transport
Peritonitis
Transforming growth factor-beta
Issue Date1999
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/AJN
Citation
American Journal Of Nephrology, 1999, v. 19 n. 3, p. 416-422 How to Cite?
AbstractRecent studies reveal conflicting results on the change of solute transfer with time on continuous ambulatory peritoneal dialysis (CAPD) and recurrent peritonitis. Herein, we performed a cross-sectional study of 76 patients on CAPD to examine their peritoneal permeability by measuring the dialysate to serum ratio of creatinine (D/P) and the mass transfer area coefficients of creatinine (MTACCr) or glucose (MTACGlu). Transforming growth factor-β1 (TGF-β1), platelet-derived growth factor (PDGF), and epidermal growth factor (EGF) were measured in the dialysate by ELISA. TGF-β1 mRNA in peritoneal macrophages were determined by a quantitative polymerase chain reaction. We failed to observe any correlation between the duration on dialysis and the peritoneal permeability in those patients with no previous peritonitis. Frequency of peritonitis episode did not affect the MTACCr, MTACGlu, or D/P. The MTACCr correlated well with MTACGlu (r = 0.78, p = 0.001) and with D/P (r = 0.98, p < 0.0001). No inverse correlation was demonstrated between dialysate PDGF or EGF and the peritoneal permeability. A positive correlation was demonstrated between the dialysate TGF-β1 and MTACCr, MTACGlu or D/P (r = 0.64, 0.54, and 0.64 respectively, p < 0.001). The dialysate TGF-β1 levels in patients with low D/P (≤ 0.5) were only half of that in patients with normal or high DIP (p = 0.0002). The dialysate levels of TGF-β1 did not correlate with PDGF or EGF. These findings raise the possibility that, other than diffusion across the peritoneal membrane from circulation, there could also be an intrinsic production of TGF-β1 by peritoneal cells in these CAPD patients. Our findings raise the speculation that TGF-β1 in dialysate from stable CAPD patients may exert an inhibitory action to fibroblast. Such action of TGF-β1 could reduce the risk of peritoneal sclerosis and hence, maintains a satisfactory peritoneal permeability to small solutes.
Persistent Identifierhttp://hdl.handle.net/10722/162330
ISSN
2015 Impact Factor: 2.605
2015 SCImago Journal Rankings: 1.308
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLai, KNen_HK
dc.contributor.authorLai, KBen_HK
dc.contributor.authorSzeto, CCen_HK
dc.contributor.authorLam, CWKen_HK
dc.contributor.authorLeung, JCKen_HK
dc.date.accessioned2012-09-05T05:19:04Z-
dc.date.available2012-09-05T05:19:04Z-
dc.date.issued1999en_HK
dc.identifier.citationAmerican Journal Of Nephrology, 1999, v. 19 n. 3, p. 416-422en_HK
dc.identifier.issn0250-8095en_HK
dc.identifier.urihttp://hdl.handle.net/10722/162330-
dc.description.abstractRecent studies reveal conflicting results on the change of solute transfer with time on continuous ambulatory peritoneal dialysis (CAPD) and recurrent peritonitis. Herein, we performed a cross-sectional study of 76 patients on CAPD to examine their peritoneal permeability by measuring the dialysate to serum ratio of creatinine (D/P) and the mass transfer area coefficients of creatinine (MTACCr) or glucose (MTACGlu). Transforming growth factor-β1 (TGF-β1), platelet-derived growth factor (PDGF), and epidermal growth factor (EGF) were measured in the dialysate by ELISA. TGF-β1 mRNA in peritoneal macrophages were determined by a quantitative polymerase chain reaction. We failed to observe any correlation between the duration on dialysis and the peritoneal permeability in those patients with no previous peritonitis. Frequency of peritonitis episode did not affect the MTACCr, MTACGlu, or D/P. The MTACCr correlated well with MTACGlu (r = 0.78, p = 0.001) and with D/P (r = 0.98, p < 0.0001). No inverse correlation was demonstrated between dialysate PDGF or EGF and the peritoneal permeability. A positive correlation was demonstrated between the dialysate TGF-β1 and MTACCr, MTACGlu or D/P (r = 0.64, 0.54, and 0.64 respectively, p < 0.001). The dialysate TGF-β1 levels in patients with low D/P (≤ 0.5) were only half of that in patients with normal or high DIP (p = 0.0002). The dialysate levels of TGF-β1 did not correlate with PDGF or EGF. These findings raise the possibility that, other than diffusion across the peritoneal membrane from circulation, there could also be an intrinsic production of TGF-β1 by peritoneal cells in these CAPD patients. Our findings raise the speculation that TGF-β1 in dialysate from stable CAPD patients may exert an inhibitory action to fibroblast. Such action of TGF-β1 could reduce the risk of peritoneal sclerosis and hence, maintains a satisfactory peritoneal permeability to small solutes.en_HK
dc.languageengen_US
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/AJNen_HK
dc.relation.ispartofAmerican Journal of Nephrologyen_HK
dc.rightsAmerican Journal of Nephrology. Copyright © S Karger AG.-
dc.subjectContinuous ambulatory peritoneal dialysisen_HK
dc.subjectMacrophagesen_HK
dc.subjectMesothelial cellsen_HK
dc.subjectPeritoneal transporten_HK
dc.subjectPeritonitisen_HK
dc.subjectTransforming growth factor-betaen_HK
dc.subject.meshBiological Transporten_US
dc.subject.meshCross-Sectional Studiesen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshFemaleen_US
dc.subject.meshGrowth Substances - Metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshKidney Failure, Chronic - Metabolism - Therapyen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshPeritoneal Dialysis, Continuous Ambulatoryen_US
dc.subject.meshPeritoneum - Metabolismen_US
dc.subject.meshPeritonitis - Metabolismen_US
dc.titleGrowth factors in continuous ambulatory peritoneal dialysis effluent: their relation with peritoneal transport of small solutesen_HK
dc.typeArticleen_HK
dc.identifier.emailLai, KN: knlai@hku.hken_HK
dc.identifier.emailLeung, JCK: jckleung@hku.hken_HK
dc.identifier.authorityLai, KN=rp00324en_HK
dc.identifier.authorityLeung, JCK=rp00448en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1159/000013488en_HK
dc.identifier.pmid10393381en_HK
dc.identifier.scopuseid_2-s2.0-0033002260en_HK
dc.identifier.hkuros41553-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033002260&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume19en_HK
dc.identifier.issue3en_HK
dc.identifier.spage416en_HK
dc.identifier.epage422en_HK
dc.identifier.isiWOS:000081320200010-
dc.publisher.placeSwitzerlanden_HK
dc.identifier.scopusauthoridLai, KN=7402135706en_HK
dc.identifier.scopusauthoridLai, KB=7402135525en_HK
dc.identifier.scopusauthoridSzeto, CC=35495407200en_HK
dc.identifier.scopusauthoridLam, CWK=8531362100en_HK
dc.identifier.scopusauthoridLeung, JCK=7202180349en_HK
dc.customcontrol.immutablejt 130530-

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