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Article: Comparison of counter immunoelectrophoresis with immunoblotting for detection of anti-extractable nuclear antigen antibodies in systemic lupus erythematosus

TitleComparison of counter immunoelectrophoresis with immunoblotting for detection of anti-extractable nuclear antigen antibodies in systemic lupus erythematosus
Authors
Issue Date1999
Citation
Asian Pacific Journal Of Allergy And Immunology, 1999, v. 17 n. 4, p. 275-279 How to Cite?
AbstractAnti-extractable nuclear antigen (ENA) antibodies were assayed by counter immunoelectrophoresis (CIE) and immunoblotting in patients with systemic lupus erythematosus (SLE). We found the two methods showed good concordance rates, the lowest being 67% for anti-SS-A. Immunoblotting was more sensitive in detecting anti-Sm, anti-SS-B and anti-PCNA (proliferating cell nuclear antigen); CIE was more sensitive for anti-nRNP and anti-SS-A. Overall, the prevalence of these anti-ENA antibodies in SLE was increased by 9-20% if immunoblotting was used in addition to CIE. Sera specific for the 52 kDa peptide of the SS-A antigen (anti-52 kDa SS-A) were better detected by immunoblotting. Anti-PCNA antibody was found in 6.3% of SLE patients and was associated with active disease and hemolytic anemia. The positive rate of anti-Sm was 9% by CIE and 23.7% by immunoblotting and this antibody was a specific marker for SLE using either method. It was concluded that using immunoblotting in addition to CIE, the overall sensitivity of detection of anti-ENA antibodies in SLE was increased and clinically useful antibodies such as anti-52 kDa SS-A and anti-PCNA could be detected.
Persistent Identifierhttp://hdl.handle.net/10722/162283
ISSN
2015 Impact Factor: 1.269
2015 SCImago Journal Rankings: 0.560
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, EYTen_US
dc.contributor.authorMok, TMYen_US
dc.contributor.authorLawton, JWMen_US
dc.contributor.authorKo, OKHen_US
dc.contributor.authorHo, Len_US
dc.contributor.authorLau, CSen_US
dc.date.accessioned2012-09-05T05:18:39Z-
dc.date.available2012-09-05T05:18:39Z-
dc.date.issued1999en_US
dc.identifier.citationAsian Pacific Journal Of Allergy And Immunology, 1999, v. 17 n. 4, p. 275-279en_US
dc.identifier.issn0125-877Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/162283-
dc.description.abstractAnti-extractable nuclear antigen (ENA) antibodies were assayed by counter immunoelectrophoresis (CIE) and immunoblotting in patients with systemic lupus erythematosus (SLE). We found the two methods showed good concordance rates, the lowest being 67% for anti-SS-A. Immunoblotting was more sensitive in detecting anti-Sm, anti-SS-B and anti-PCNA (proliferating cell nuclear antigen); CIE was more sensitive for anti-nRNP and anti-SS-A. Overall, the prevalence of these anti-ENA antibodies in SLE was increased by 9-20% if immunoblotting was used in addition to CIE. Sera specific for the 52 kDa peptide of the SS-A antigen (anti-52 kDa SS-A) were better detected by immunoblotting. Anti-PCNA antibody was found in 6.3% of SLE patients and was associated with active disease and hemolytic anemia. The positive rate of anti-Sm was 9% by CIE and 23.7% by immunoblotting and this antibody was a specific marker for SLE using either method. It was concluded that using immunoblotting in addition to CIE, the overall sensitivity of detection of anti-ENA antibodies in SLE was increased and clinically useful antibodies such as anti-52 kDa SS-A and anti-PCNA could be detected.en_US
dc.languageengen_US
dc.relation.ispartofAsian Pacific Journal of Allergy and Immunologyen_US
dc.subject.meshAnemia, Hemolytic - Blood - Complicationsen_US
dc.subject.meshAntibodies, Antinuclear - Analysisen_US
dc.subject.meshAntibody Specificity - Immunologyen_US
dc.subject.meshAutoantigens - Immunologyen_US
dc.subject.meshBiological Markers - Blooden_US
dc.subject.meshDisease Progressionen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoblottingen_US
dc.subject.meshImmunoelectrophoresisen_US
dc.subject.meshLupus Erythematosus, Systemic - Blood - Immunologyen_US
dc.subject.meshProliferating Cell Nuclear Antigen - Immunologyen_US
dc.subject.meshRna, Small Cytoplasmicen_US
dc.subject.meshRibonucleoproteins - Immunologyen_US
dc.subject.meshRibonucleoproteins, Small Nuclearen_US
dc.subject.meshSensitivity And Specificityen_US
dc.subject.meshSnrnp Core Proteinsen_US
dc.titleComparison of counter immunoelectrophoresis with immunoblotting for detection of anti-extractable nuclear antigen antibodies in systemic lupus erythematosusen_US
dc.typeArticleen_US
dc.identifier.emailMok, TMY:temy@hkucc.hku.hken_US
dc.identifier.emailLau, CS:cslau@hku.hken_US
dc.identifier.authorityMok, TMY=rp00490en_US
dc.identifier.authorityLau, CS=rp01348en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid10698467-
dc.identifier.scopuseid_2-s2.0-0032718433en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032718433&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume17en_US
dc.identifier.issue4en_US
dc.identifier.spage275en_US
dc.identifier.epage279en_US
dc.identifier.isiWOS:000084068100006-
dc.publisher.placeThailanden_US
dc.identifier.scopusauthoridChan, EYT=7401994013en_US
dc.identifier.scopusauthoridMok, TMY=7006024184en_US
dc.identifier.scopusauthoridLawton, JWM=7102180792en_US
dc.identifier.scopusauthoridKo, OKH=36855540800en_US
dc.identifier.scopusauthoridHo, L=8119962100en_US
dc.identifier.scopusauthoridLau, CS=14035682100en_US

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