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Article: Muscarinic receptor subtypes in the porcine lung during postnatal development

TitleMuscarinic receptor subtypes in the porcine lung during postnatal development
Authors
Issue Date1998
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ejphar
Citation
European Journal Of Pharmacology, 1998, v. 359 n. 2-3, p. 211-221 How to Cite?
AbstractThe responsiveness of the pulmonary circulation to acetylcholine changes in the newborn piglet. Therefore muscarinic receptors have been studied in the developing porcine lung from birth to adulthood using ligand binding, Northern blotting and in situ hybridisation. Maximal binding capacity of [N-methyl- 3H] scopolamine and the affinity of the receptor in lung membranes increased between birth and 16 days (p<0.05). Subtype affinity changed with age, but always M 3>M 1>M 2. Northern blots of porcine muscarinic receptor subtypes showed m1, m2 and m3 mRNA present in lung membranes. m2 mRNA was present at all ages and decreased with age. m1 mRNA was absent at birth and m3 mRNA was absent at 3 days. Autoradiographic localisation showed ligand binding to the parenchyma and airway smooth muscle and nerves, there was no binding to intrapulmonary vessels. In situ hybridisation localised mRNA of all three subtypes to the smooth muscle cells of both vessels and airways. Changes in the receptor subtypes may explain the pharmacological changes during postnatal adaptation. Copyright (C) 1998 Elsevier Science B.V.
Persistent Identifierhttp://hdl.handle.net/10722/162266
ISSN
2015 Impact Factor: 2.73
2015 SCImago Journal Rankings: 1.115
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHislop, AAen_US
dc.contributor.authorMak, JCWen_US
dc.contributor.authorReader, JAen_US
dc.contributor.authorBarnes, PJen_US
dc.contributor.authorHaworth, SGen_US
dc.date.accessioned2012-09-05T05:18:30Z-
dc.date.available2012-09-05T05:18:30Z-
dc.date.issued1998en_US
dc.identifier.citationEuropean Journal Of Pharmacology, 1998, v. 359 n. 2-3, p. 211-221en_US
dc.identifier.issn0014-2999en_US
dc.identifier.urihttp://hdl.handle.net/10722/162266-
dc.description.abstractThe responsiveness of the pulmonary circulation to acetylcholine changes in the newborn piglet. Therefore muscarinic receptors have been studied in the developing porcine lung from birth to adulthood using ligand binding, Northern blotting and in situ hybridisation. Maximal binding capacity of [N-methyl- 3H] scopolamine and the affinity of the receptor in lung membranes increased between birth and 16 days (p<0.05). Subtype affinity changed with age, but always M 3>M 1>M 2. Northern blots of porcine muscarinic receptor subtypes showed m1, m2 and m3 mRNA present in lung membranes. m2 mRNA was present at all ages and decreased with age. m1 mRNA was absent at birth and m3 mRNA was absent at 3 days. Autoradiographic localisation showed ligand binding to the parenchyma and airway smooth muscle and nerves, there was no binding to intrapulmonary vessels. In situ hybridisation localised mRNA of all three subtypes to the smooth muscle cells of both vessels and airways. Changes in the receptor subtypes may explain the pharmacological changes during postnatal adaptation. Copyright (C) 1998 Elsevier Science B.V.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ejpharen_US
dc.relation.ispartofEuropean Journal of Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnimals, Newbornen_US
dc.subject.meshBlotting, Northernen_US
dc.subject.meshGene Expression Regulation, Developmentalen_US
dc.subject.meshIn Situ Hybridizationen_US
dc.subject.meshLung - Growth & Development - Metabolismen_US
dc.subject.meshRna, Messenger - Analysis - Geneticsen_US
dc.subject.meshRadioligand Assayen_US
dc.subject.meshReceptors, Muscarinic - Genetics - Metabolismen_US
dc.subject.meshSwineen_US
dc.titleMuscarinic receptor subtypes in the porcine lung during postnatal developmenten_US
dc.typeArticleen_US
dc.identifier.emailMak, JCW:judymak@hku.hken_US
dc.identifier.authorityMak, JCW=rp00352en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0014-2999(98)00585-8en_US
dc.identifier.pmid9832393-
dc.identifier.scopuseid_2-s2.0-0032561331en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032561331&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume359en_US
dc.identifier.issue2-3en_US
dc.identifier.spage211en_US
dc.identifier.epage221en_US
dc.identifier.isiWOS:000076840200015-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridHislop, AA=7005801811en_US
dc.identifier.scopusauthoridMak, JCW=7103323094en_US
dc.identifier.scopusauthoridReader, JA=55160661300en_US
dc.identifier.scopusauthoridBarnes, PJ=36064679400en_US
dc.identifier.scopusauthoridHaworth, SG=35513064700en_US

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