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Article: Characterization of a transient outward K + current with inward rectification in canine ventricular myocytes

TitleCharacterization of a transient outward K + current with inward rectification in canine ventricular myocytes
Authors
Issue Date1998
PublisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpcell.physiology.org/
Citation
American Journal Of Physiology - Cell Physiology, 1998, v. 274 n. 3 43-3, p. C577-C585 How to Cite?
AbstractThe threshold potential for the classical depolarization-activated transient outward K + current and Cl - current is positive to -30 mV. With the whole cell patch technique, a transient outward current was elicited in the presence of 5 mM 4-aminopyridine (4-AP) and 5 μM ryanodine at voltages positive to the K + equilibrium potential in canine ventricular myocytes. The current was abolished by 200 μM Ba 2+ or omission of external K + (K 0 +) and showed biexponential inactivation. The current-voltage relation for the peak of the transient outward component showed moderate inward rectification. The transient outward current demonstrated voltage-dependent inactivation (half-inactivation voltage: -43.5 ± 3.2 mV) and rapid, monoexponential recovery from inactivation (time constant: 13.2 ± 2.5 ms). The reversal potential responded to the changes in K 0 + concentration. Action potential clamp revealed two phases of Ba 2+-sensitive current during the action potential, including a large early transient component after the upstroke and a later outward component during phase 3 repolarization. The present study demonstrates that depolarization may elicit a Ba 2+-and K 0 +-sensitive, 4- AP-insensitive, transient outward current with inward rectification in canine ventricular myocytes. The properties of this K + current suggest that it may carry a significant early outward current upon depolarization that may play a role in determining membrane excitability and action potential morphology.
Persistent Identifierhttp://hdl.handle.net/10722/162246
ISSN
2015 Impact Factor: 3.395
2015 SCImago Journal Rankings: 1.893
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, GRen_US
dc.contributor.authorSun, Hen_US
dc.contributor.authorNattel, Sen_US
dc.date.accessioned2012-09-05T05:18:22Z-
dc.date.available2012-09-05T05:18:22Z-
dc.date.issued1998en_US
dc.identifier.citationAmerican Journal Of Physiology - Cell Physiology, 1998, v. 274 n. 3 43-3, p. C577-C585en_US
dc.identifier.issn0363-6143en_US
dc.identifier.urihttp://hdl.handle.net/10722/162246-
dc.description.abstractThe threshold potential for the classical depolarization-activated transient outward K + current and Cl - current is positive to -30 mV. With the whole cell patch technique, a transient outward current was elicited in the presence of 5 mM 4-aminopyridine (4-AP) and 5 μM ryanodine at voltages positive to the K + equilibrium potential in canine ventricular myocytes. The current was abolished by 200 μM Ba 2+ or omission of external K + (K 0 +) and showed biexponential inactivation. The current-voltage relation for the peak of the transient outward component showed moderate inward rectification. The transient outward current demonstrated voltage-dependent inactivation (half-inactivation voltage: -43.5 ± 3.2 mV) and rapid, monoexponential recovery from inactivation (time constant: 13.2 ± 2.5 ms). The reversal potential responded to the changes in K 0 + concentration. Action potential clamp revealed two phases of Ba 2+-sensitive current during the action potential, including a large early transient component after the upstroke and a later outward component during phase 3 repolarization. The present study demonstrates that depolarization may elicit a Ba 2+-and K 0 +-sensitive, 4- AP-insensitive, transient outward current with inward rectification in canine ventricular myocytes. The properties of this K + current suggest that it may carry a significant early outward current upon depolarization that may play a role in determining membrane excitability and action potential morphology.en_US
dc.languageengen_US
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpcell.physiology.org/en_US
dc.relation.ispartofAmerican Journal of Physiology - Cell Physiologyen_US
dc.subject.meshAction Potentialsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBarium - Metabolismen_US
dc.subject.meshDelayed Rectifier Potassium Channelsen_US
dc.subject.meshDogsen_US
dc.subject.meshElectrophysiologyen_US
dc.subject.meshHeart Ventricles - Cytologyen_US
dc.subject.meshKineticsen_US
dc.subject.meshMagnesium - Metabolismen_US
dc.subject.meshMyocardium - Metabolismen_US
dc.subject.meshPotassium - Metabolismen_US
dc.subject.meshPotassium Channels - Drug Effects - Metabolism - Physiologyen_US
dc.subject.meshPotassium Channels, Inwardly Rectifyingen_US
dc.subject.meshPotassium Channels, Voltage-Gateden_US
dc.subject.meshPyrimidines - Pharmacologyen_US
dc.subject.meshSpermine - Metabolismen_US
dc.titleCharacterization of a transient outward K + current with inward rectification in canine ventricular myocytesen_US
dc.typeArticleen_US
dc.identifier.emailLi, GR:grli@hkucc.hku.hken_US
dc.identifier.authorityLi, GR=rp00476en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid9530088-
dc.identifier.scopuseid_2-s2.0-0031981155en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031981155&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume274en_US
dc.identifier.issue3 43-3en_US
dc.identifier.spageC577en_US
dc.identifier.epageC585en_US
dc.identifier.isiWOS:000072382500004-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLi, GR=7408462932en_US
dc.identifier.scopusauthoridSun, H=35723049200en_US
dc.identifier.scopusauthoridNattel, S=36947837400en_US

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