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Article: Gene expression of the renin-angiotensin system in human kidney

TitleGene expression of the renin-angiotensin system in human kidney
Authors
KeywordsAngiotensin converting enzyme
Angiotensinogen
Human glomerulus
mRNA
Renin
Issue Date1998
PublisherLippincott Williams & Wilkins, Ltd. The Journal's web site is located at http://www.jhypertension.com/
Citation
Journal Of Hypertension, 1998, v. 16 n. 1, p. 91-102 How to Cite?
AbstractBackground. Immunocytochemical studies have revealed that all components of the renin-angiotensin system are widely distributed in human tissues yet the information on the gene expression of the renin-angiotensin system in various types of cell remains scarce. Objective. We explored the presence of a local renin-angiotensin system in human kidney. Methods. We sought to determine the presence of messenger RNA (mRNA) encoding for renin, angiotensinogen, and angiotensin converting enzyme (ACE) in cultured human glomerular cells and human umbilical vein endothelial cells using a two-step polymerase chain reaction. The gene expression of the renin-angiotensin system in normal human kidney and in diseased kidney was studied by in-situ hybridization using synthetic oligonucleotides. Results. By using a two-step polymerase chain reaction, renin, angiotensinogen, and ACE mRNA were found in cultured mesangial and epithelial cells but only ACE mRNA was present in human umbilical vein endothelial cells. Renin mRNA was detected in juxtaglomerular granular cells and also in glomerular and tubular epithelia in normal kidney by in-situ hybridization. A similar tubular, but not mesangial, distribution was found with angiotensinogen and ACE mRNA. In contrast, stronger signals for renin, angiotensinogen and ACE mRNA were detected in mesangial and epithelial cells of kidney tissues from hypertensive patients and from patients with renal pathology characterized by mesangial proliferation (immunoglobulin A nephropathy, diabetes mellitus, or lupus nephritis). Conclusions. That gene expression of the renin-angiotensin system occurs in resident glomerular cells supports the hypothesis that there is a local renin-angiotensin system in human kidney. Our findings support the previous speculation that the renin-angiotensin system could be a local factor involved in the progression of chronic renal failure and consequent development of hypertension.
Persistent Identifierhttp://hdl.handle.net/10722/162240
ISSN
2015 Impact Factor: 5.062
2015 SCImago Journal Rankings: 2.193
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLai, KNen_HK
dc.contributor.authorLeung, JCKen_HK
dc.contributor.authorLai, KBen_HK
dc.contributor.authorTo, WYen_HK
dc.contributor.authorYeung, VTFen_HK
dc.contributor.authorLai, FMMen_HK
dc.date.accessioned2012-09-05T05:18:20Z-
dc.date.available2012-09-05T05:18:20Z-
dc.date.issued1998en_HK
dc.identifier.citationJournal Of Hypertension, 1998, v. 16 n. 1, p. 91-102en_HK
dc.identifier.issn0263-6352en_HK
dc.identifier.urihttp://hdl.handle.net/10722/162240-
dc.description.abstractBackground. Immunocytochemical studies have revealed that all components of the renin-angiotensin system are widely distributed in human tissues yet the information on the gene expression of the renin-angiotensin system in various types of cell remains scarce. Objective. We explored the presence of a local renin-angiotensin system in human kidney. Methods. We sought to determine the presence of messenger RNA (mRNA) encoding for renin, angiotensinogen, and angiotensin converting enzyme (ACE) in cultured human glomerular cells and human umbilical vein endothelial cells using a two-step polymerase chain reaction. The gene expression of the renin-angiotensin system in normal human kidney and in diseased kidney was studied by in-situ hybridization using synthetic oligonucleotides. Results. By using a two-step polymerase chain reaction, renin, angiotensinogen, and ACE mRNA were found in cultured mesangial and epithelial cells but only ACE mRNA was present in human umbilical vein endothelial cells. Renin mRNA was detected in juxtaglomerular granular cells and also in glomerular and tubular epithelia in normal kidney by in-situ hybridization. A similar tubular, but not mesangial, distribution was found with angiotensinogen and ACE mRNA. In contrast, stronger signals for renin, angiotensinogen and ACE mRNA were detected in mesangial and epithelial cells of kidney tissues from hypertensive patients and from patients with renal pathology characterized by mesangial proliferation (immunoglobulin A nephropathy, diabetes mellitus, or lupus nephritis). Conclusions. That gene expression of the renin-angiotensin system occurs in resident glomerular cells supports the hypothesis that there is a local renin-angiotensin system in human kidney. Our findings support the previous speculation that the renin-angiotensin system could be a local factor involved in the progression of chronic renal failure and consequent development of hypertension.en_HK
dc.languageengen_US
dc.publisherLippincott Williams & Wilkins, Ltd. The Journal's web site is located at http://www.jhypertension.com/en_HK
dc.relation.ispartofJournal of Hypertensionen_HK
dc.rightsJournal of Hypertension. Copyright © Lippincott Williams & Wilkins, Ltd.-
dc.subjectAngiotensin converting enzymeen_HK
dc.subjectAngiotensinogenen_HK
dc.subjectHuman glomerulusen_HK
dc.subjectmRNAen_HK
dc.subjectReninen_HK
dc.subject.meshAngiotensinogen - Geneticsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshDna Primers - Geneticsen_US
dc.subject.meshGene Expressionen_US
dc.subject.meshHumansen_US
dc.subject.meshIn Situ Hybridizationen_US
dc.subject.meshKidney - Metabolismen_US
dc.subject.meshKidney Diseases - Genetics - Metabolismen_US
dc.subject.meshKidney Glomerulus - Cytology - Metabolismen_US
dc.subject.meshPeptidyl-Dipeptidase A - Geneticsen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshRna, Messenger - Genetics - Metabolismen_US
dc.subject.meshRenin - Geneticsen_US
dc.subject.meshRenin-Angiotensin System - Genetics - Physiologyen_US
dc.titleGene expression of the renin-angiotensin system in human kidneyen_HK
dc.typeArticleen_HK
dc.identifier.emailLai, KN: knlai@hku.hken_HK
dc.identifier.emailLeung, JCK: jckleung@hku.hken_HK
dc.identifier.authorityLai, KN=rp00324en_HK
dc.identifier.authorityLeung, JCK=rp00448en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1097/00004872-199816010-00014en_HK
dc.identifier.pmid9533422-
dc.identifier.scopuseid_2-s2.0-0031890271en_HK
dc.identifier.hkuros41501-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031890271&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume16en_HK
dc.identifier.issue1en_HK
dc.identifier.spage91en_HK
dc.identifier.epage102en_HK
dc.identifier.isiWOS:000071700500014-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLai, KN=7402135706en_HK
dc.identifier.scopusauthoridLeung, JCK=7202180349en_HK
dc.identifier.scopusauthoridLai, KB=7402135525en_HK
dc.identifier.scopusauthoridTo, WY=36933508500en_HK
dc.identifier.scopusauthoridYeung, VTF=35892061300en_HK
dc.identifier.scopusauthoridLai, FMM=7202559720en_HK

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