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Article: Extracellular expression of human epidermal growth factor encoded by an Escherichia coli K-12 plasmid stabilized by the ytl2-incR system of Salmonella typhimurium

TitleExtracellular expression of human epidermal growth factor encoded by an Escherichia coli K-12 plasmid stabilized by the ytl2-incR system of Salmonella typhimurium
Authors
KeywordsHegf
Plasmid Pslt
Ytl2-Incr Stabilization
Issue Date1998
Citation
Journal Of Industrial Microbiology And Biotechnology, 1998, v. 21 n. 1-2, p. 31-36 How to Cite?
AbstractA plasmid stabilization system, active in high copy-number plasmids, was cloned from the large resident plasmid, pSLT, of Salmonella typhimurium. The ytl2 gene, together with a 249-bp region (termed incR) downstream of the gene, imparted > 10 4-fold stability to a pBR322-based plasmid. The ytl2-incR region was then used to stabilize a recombinant plasmid carrying the human epidermal growth factor gene (with the Escherichia coli K-12 ompA signal sequence), behind the lacUV5 promoter. In shake flask tests to optimize expression of human epidermal growth factor, loss of recombinant plasmid was < 1% when growth (both before and after induction with isopropyl-β-D-galactopyranoside) took place even in the absence of antibiotic selection, and the specific activity of secreted human epidermal growth factor was ca 20 μg per 10 8 cells at harvest, compared to a figure of ca 3 μg per 10 8 cells when a comparable plasmid, but devoid of the ytl2-incR region, was employed, as outgrowth of plasmid-free cells after induction severely compromised the specific activity of the secreted product.
Persistent Identifierhttp://hdl.handle.net/10722/162231
ISSN
2015 Impact Factor: 2.745
2015 SCImago Journal Rankings: 0.966
References

 

DC FieldValueLanguage
dc.contributor.authorWong, DKHen_US
dc.contributor.authorLam, KHEen_US
dc.contributor.authorChan, CKPen_US
dc.contributor.authorWong, YCVen_US
dc.contributor.authorWong, WKRen_US
dc.contributor.authorHackett, Jen_US
dc.date.accessioned2012-09-05T05:18:17Z-
dc.date.available2012-09-05T05:18:17Z-
dc.date.issued1998en_US
dc.identifier.citationJournal Of Industrial Microbiology And Biotechnology, 1998, v. 21 n. 1-2, p. 31-36en_US
dc.identifier.issn1367-5435en_US
dc.identifier.urihttp://hdl.handle.net/10722/162231-
dc.description.abstractA plasmid stabilization system, active in high copy-number plasmids, was cloned from the large resident plasmid, pSLT, of Salmonella typhimurium. The ytl2 gene, together with a 249-bp region (termed incR) downstream of the gene, imparted > 10 4-fold stability to a pBR322-based plasmid. The ytl2-incR region was then used to stabilize a recombinant plasmid carrying the human epidermal growth factor gene (with the Escherichia coli K-12 ompA signal sequence), behind the lacUV5 promoter. In shake flask tests to optimize expression of human epidermal growth factor, loss of recombinant plasmid was < 1% when growth (both before and after induction with isopropyl-β-D-galactopyranoside) took place even in the absence of antibiotic selection, and the specific activity of secreted human epidermal growth factor was ca 20 μg per 10 8 cells at harvest, compared to a figure of ca 3 μg per 10 8 cells when a comparable plasmid, but devoid of the ytl2-incR region, was employed, as outgrowth of plasmid-free cells after induction severely compromised the specific activity of the secreted product.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Industrial Microbiology and Biotechnologyen_US
dc.subjectHegfen_US
dc.subjectPlasmid Pslten_US
dc.subjectYtl2-Incr Stabilizationen_US
dc.titleExtracellular expression of human epidermal growth factor encoded by an Escherichia coli K-12 plasmid stabilized by the ytl2-incR system of Salmonella typhimuriumen_US
dc.typeArticleen_US
dc.identifier.emailWong, DKH:danywong@hku.hken_US
dc.identifier.authorityWong, DKH=rp00492en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-0031797633en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031797633&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume21en_US
dc.identifier.issue1-2en_US
dc.identifier.spage31en_US
dc.identifier.epage36en_US
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridWong, DKH=7401535819en_US
dc.identifier.scopusauthoridLam, KHE=36919954500en_US
dc.identifier.scopusauthoridChan, CKP=7404813824en_US
dc.identifier.scopusauthoridWong, YCV=36853087700en_US
dc.identifier.scopusauthoridWong, WKR=7403972071en_US
dc.identifier.scopusauthoridHackett, J=24302040000en_US

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