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Article: Evaluation of a selective transport medium for gastric biopsy specimens to be cultured for Helicobacter pylori

TitleEvaluation of a selective transport medium for gastric biopsy specimens to be cultured for Helicobacter pylori
Authors
Issue Date1998
Citation
Journal Of Clinical Microbiology, 1998, v. 36 n. 10, p. 3048-3050 How to Cite?
AbstractSince the means of culturing Helicobacter pylori may not be available in some laboratories, prolonging the survival of this organism during transportation is a major concern in terms of improving detection rates. A selective transport medium was evaluated for the preservation of H. pylori from 254 gastric biopsy specimens collected from a rural area in China where culturing is not feasible. Gastric biopsy specimens were inoculated in sterile broth consisting of brain heart infusion (BHI) broth, horse serum, and yeast extract supplemented with vancomycin, amphotericin B, and nalidixic acid (VAN). Of the 254 biopsy specimens, 238 were identified by histology to have H. pylori infection. Total rates of recovery of H. pylori from the H. pylori-positive gastric biopsy specimens stored in the BHI-VAN broth ranged from 76 to 46% after storage of specimens for 5 to 9 days. In conclusion, the selective medium is useful for prolonging the survival of H. pylori in gastric biopsy specimens for which immediate culture is not feasible.
Persistent Identifierhttp://hdl.handle.net/10722/162220
ISSN
2015 Impact Factor: 3.631
2015 SCImago Journal Rankings: 2.151
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSiu, LKen_US
dc.contributor.authorLeung, WKen_US
dc.contributor.authorCheng, AFBen_US
dc.contributor.authorSung, JYen_US
dc.contributor.authorLing, TKWen_US
dc.contributor.authorLing, JMLen_US
dc.contributor.authorNg, EKWen_US
dc.contributor.authorLau, JYWen_US
dc.contributor.authorChung, SCSen_US
dc.date.accessioned2012-09-05T05:18:12Z-
dc.date.available2012-09-05T05:18:12Z-
dc.date.issued1998en_US
dc.identifier.citationJournal Of Clinical Microbiology, 1998, v. 36 n. 10, p. 3048-3050en_US
dc.identifier.issn0095-1137en_US
dc.identifier.urihttp://hdl.handle.net/10722/162220-
dc.description.abstractSince the means of culturing Helicobacter pylori may not be available in some laboratories, prolonging the survival of this organism during transportation is a major concern in terms of improving detection rates. A selective transport medium was evaluated for the preservation of H. pylori from 254 gastric biopsy specimens collected from a rural area in China where culturing is not feasible. Gastric biopsy specimens were inoculated in sterile broth consisting of brain heart infusion (BHI) broth, horse serum, and yeast extract supplemented with vancomycin, amphotericin B, and nalidixic acid (VAN). Of the 254 biopsy specimens, 238 were identified by histology to have H. pylori infection. Total rates of recovery of H. pylori from the H. pylori-positive gastric biopsy specimens stored in the BHI-VAN broth ranged from 76 to 46% after storage of specimens for 5 to 9 days. In conclusion, the selective medium is useful for prolonging the survival of H. pylori in gastric biopsy specimens for which immediate culture is not feasible.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Clinical Microbiologyen_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAmoxicillin - Therapeutic Useen_US
dc.subject.meshAnti-Bacterial Agents - Therapeutic Useen_US
dc.subject.meshAnti-Ulcer Agents - Therapeutic Useen_US
dc.subject.meshBiopsyen_US
dc.subject.meshChinaen_US
dc.subject.meshClarithromycin - Therapeutic Useen_US
dc.subject.meshCulture Mediaen_US
dc.subject.meshFemaleen_US
dc.subject.meshGastric Mucosa - Microbiology - Pathologyen_US
dc.subject.meshHelicobacter Infections - Drug Therapy - Pathology - Prevention & Controlen_US
dc.subject.meshHelicobacter Pylori - Growth & Development - Isolation & Purificationen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshOmeprazole - Therapeutic Useen_US
dc.subject.meshPenicillins - Therapeutic Useen_US
dc.subject.meshPilot Projectsen_US
dc.subject.meshProspective Studiesen_US
dc.subject.meshRural Healthen_US
dc.subject.meshStomach - Microbiology - Pathologyen_US
dc.titleEvaluation of a selective transport medium for gastric biopsy specimens to be cultured for Helicobacter pylorien_US
dc.typeArticleen_US
dc.identifier.emailLeung, WK:waikleung@hku.hken_US
dc.identifier.authorityLeung, WK=rp01479en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid9738066-
dc.identifier.scopuseid_2-s2.0-0031683627en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031683627&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume36en_US
dc.identifier.issue10en_US
dc.identifier.spage3048en_US
dc.identifier.epage3050en_US
dc.identifier.isiWOS:000075896800043-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridSiu, LK=55231066500en_US
dc.identifier.scopusauthoridLeung, WK=7201504523en_US
dc.identifier.scopusauthoridCheng, AFB=7402075035en_US
dc.identifier.scopusauthoridSung, JY=8978634400en_US
dc.identifier.scopusauthoridLing, TKW=13310253900en_US
dc.identifier.scopusauthoridLing, JML=7201776378en_US
dc.identifier.scopusauthoridNg, EKW=7201647539en_US
dc.identifier.scopusauthoridLau, JYW=13907867100en_US
dc.identifier.scopusauthoridChung, SCS=19642462800en_US

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