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Article: Effect of anti-DNA autoantibodies on the gene expression of interleukin 8, transforming growth factor-β, and nitric oxide synthase in cultured endothelial cells

TitleEffect of anti-DNA autoantibodies on the gene expression of interleukin 8, transforming growth factor-β, and nitric oxide synthase in cultured endothelial cells
Authors
KeywordsAngiogenesis
Anti-DNA autoantibodies
Apoptosis
Endothelial cells
Inducible nitric oxide synthase
Interleukin 8
Systemic lupus erythematosus
Transforming growth factor-β
Issue Date1997
PublisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/03009742.asp
Citation
Scandinavian Journal Of Rheumatology, 1997, v. 26 n. 6, p. 461-467 How to Cite?
AbstractOur study aims to determine whether anti-dsDNA exerts any effect on the gene expression of IL-8 or TGF-β in cultured HUVEC. Both cytokines have angiogenic effect on endothelial cells. IgG was purified from 19 patients with SLE and from 19 healthy controls. Anti-dsDNA-depleted polyclonal IgG was also prepared from serum IgG of lupus patients by affinity chromatography with DNA cellulose column. Compared with either control IgG or anti-dsDNA- dep-IgG, HUVEC incubated with anti-dsDNA-containing-IgG expressed higher levels of IL-8 mRNA (p=0.0001) and TGF-β1 mRNA (p=0.0014). We demonstrated a significant increase in the percentage of cells with fragmented DNA in HUVEC incubated with anti-dsDNA-containing-IgG compared with those incubated with anti-dsDNA-dep-IgG, supporting the notion that anti-dsDNA may exert a direct apoptotic effect on cultured endothelial cells. Our study provides in vitro evidence that anti-dsDNA could play an important pathogenetic role in inducing inflammatory injury of vascular endothelium in SLE.
Persistent Identifierhttp://hdl.handle.net/10722/162215
ISSN
2015 Impact Factor: 2.307
2015 SCImago Journal Rankings: 1.125
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLai, KNen_HK
dc.contributor.authorLeung, JCKen_HK
dc.contributor.authorLai, KBen_HK
dc.contributor.authorLai, CKWen_HK
dc.date.accessioned2012-09-05T05:18:10Z-
dc.date.available2012-09-05T05:18:10Z-
dc.date.issued1997en_HK
dc.identifier.citationScandinavian Journal Of Rheumatology, 1997, v. 26 n. 6, p. 461-467en_HK
dc.identifier.issn0300-9742en_HK
dc.identifier.urihttp://hdl.handle.net/10722/162215-
dc.description.abstractOur study aims to determine whether anti-dsDNA exerts any effect on the gene expression of IL-8 or TGF-β in cultured HUVEC. Both cytokines have angiogenic effect on endothelial cells. IgG was purified from 19 patients with SLE and from 19 healthy controls. Anti-dsDNA-depleted polyclonal IgG was also prepared from serum IgG of lupus patients by affinity chromatography with DNA cellulose column. Compared with either control IgG or anti-dsDNA- dep-IgG, HUVEC incubated with anti-dsDNA-containing-IgG expressed higher levels of IL-8 mRNA (p=0.0001) and TGF-β1 mRNA (p=0.0014). We demonstrated a significant increase in the percentage of cells with fragmented DNA in HUVEC incubated with anti-dsDNA-containing-IgG compared with those incubated with anti-dsDNA-dep-IgG, supporting the notion that anti-dsDNA may exert a direct apoptotic effect on cultured endothelial cells. Our study provides in vitro evidence that anti-dsDNA could play an important pathogenetic role in inducing inflammatory injury of vascular endothelium in SLE.en_HK
dc.languageengen_US
dc.publisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/03009742.aspen_HK
dc.relation.ispartofScandinavian Journal of Rheumatologyen_HK
dc.rightsScandinavian Journal of Rheumatology. Copyright © Informa Healthcare.-
dc.subjectAngiogenesisen_HK
dc.subjectAnti-DNA autoantibodiesen_HK
dc.subjectApoptosisen_HK
dc.subjectEndothelial cellsen_HK
dc.subjectInducible nitric oxide synthaseen_HK
dc.subjectInterleukin 8en_HK
dc.subjectSystemic lupus erythematosusen_HK
dc.subjectTransforming growth factor-βen_HK
dc.subject.meshAdulten_US
dc.subject.meshAntibodies, Antinuclear - Pharmacologyen_US
dc.subject.meshApoptosis - Immunologyen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshDna - Immunologyen_US
dc.subject.meshDna Fragmentationen_US
dc.subject.meshEndothelium, Vascular - Cytology - Enzymology - Immunologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshFlow Cytometryen_US
dc.subject.meshGene Expression Regulation - Immunologyen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoglobulin G - Pharmacologyen_US
dc.subject.meshInterleukin-8 - Geneticsen_US
dc.subject.meshLupus Erythematosus, Systemic - Immunologyen_US
dc.subject.meshMaleen_US
dc.subject.meshNitric Oxide Synthase - Geneticsen_US
dc.subject.meshRna, Messenger - Analysisen_US
dc.subject.meshTransforming Growth Factor Beta - Geneticsen_US
dc.subject.meshUmbilical Veins - Cytologyen_US
dc.titleEffect of anti-DNA autoantibodies on the gene expression of interleukin 8, transforming growth factor-β, and nitric oxide synthase in cultured endothelial cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailLai, KN: knlai@hku.hken_HK
dc.identifier.emailLeung, JCK: jckleung@hku.hken_HK
dc.identifier.authorityLai, KN=rp00324en_HK
dc.identifier.authorityLeung, JCK=rp00448en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.3109/03009749709065720-
dc.identifier.pmid9433408-
dc.identifier.scopuseid_2-s2.0-0031452106en_HK
dc.identifier.hkuros41499-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031452106&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume26en_HK
dc.identifier.issue6en_HK
dc.identifier.spage461en_HK
dc.identifier.epage467en_HK
dc.identifier.isiWOS:A1997YL08200010-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLai, KN=7402135706en_HK
dc.identifier.scopusauthoridLeung, JCK=7202180349en_HK
dc.identifier.scopusauthoridLai, KB=7402135525en_HK
dc.identifier.scopusauthoridLai, CKW=7403086390en_HK

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