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Article: Upregulation of adhesion molecule expression on endothelial cells by anti-DNA autoantibodies in systemic lupus erythematosus

TitleUpregulation of adhesion molecule expression on endothelial cells by anti-DNA autoantibodies in systemic lupus erythematosus
Authors
Issue Date1996
Citation
Clinical Immunology And Immunopathology, 1996, v. 81 n. 3, p. 229-238 How to Cite?
AbstractThe mechanism of vasculopathy in systemic lupus erythematosus (SLE) remains unclear and the evidence for a direct pathogenic role of anti-double-stranded DNA antibodies (anti-dsDNA) is not strong. Our study aims to determine whether anti-dsDNA exerts any effect on the expression of adhesion molecules on endothelial cells. IgG was purified from 17 patients with SLE (median anti-dsDNA titer, 404 IU/ml) and from 9 healthy controls (median titer 16 IU/ml). Anti-dsDNA depleted polyclonal IgG (anti-dsDNA-dep-IgG) (median anti-dsDNA titer 17 IU/ml) was prepared from sera of these patients with SLE by affinity chromatography with DNA cellulose column. Expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin on human umbilical vein endothelial cells (HUVEC) cultured with either control IgG or anti-dsDNA were compared by how cytometry. The levels of adhesion molecules in the supernatant of cultured HUVEC were assessed by sandwich ELISA. Compared with either control IgG or anti-dsDNA dep-IgG, HUVEC incubated with anti-dsDNA expressed a significantly higher mean fluorescence intensity of ICAM-1 and in VCAM-1 and a higher supernatant concentration of ICAM-1 and VCAM-1 but not E-selectin. At the same time, ICAM-1 mRNA was also raised with increased neutrophil adherence in HUVEC incubated with anti-dsDNA. Pretreatment of HUVEC with native DNA or histone before incubation with anti-dsDNA did not increase the expression of adhesion molecules. Our study provides in vitro evidence that anti-dsDNA could play an important pathogenic role in inducing inflammatory injury of vascular endothelium in SLE.
Persistent Identifierhttp://hdl.handle.net/10722/162166
ISSN
1998 Impact Factor: 2.047
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLai, KNen_HK
dc.contributor.authorLeung, JCKen_HK
dc.contributor.authorLai, KBen_HK
dc.contributor.authorWong, KCen_HK
dc.contributor.authorLai, CKWen_HK
dc.date.accessioned2012-09-05T05:17:46Z-
dc.date.available2012-09-05T05:17:46Z-
dc.date.issued1996en_HK
dc.identifier.citationClinical Immunology And Immunopathology, 1996, v. 81 n. 3, p. 229-238en_HK
dc.identifier.issn0090-1229en_HK
dc.identifier.urihttp://hdl.handle.net/10722/162166-
dc.description.abstractThe mechanism of vasculopathy in systemic lupus erythematosus (SLE) remains unclear and the evidence for a direct pathogenic role of anti-double-stranded DNA antibodies (anti-dsDNA) is not strong. Our study aims to determine whether anti-dsDNA exerts any effect on the expression of adhesion molecules on endothelial cells. IgG was purified from 17 patients with SLE (median anti-dsDNA titer, 404 IU/ml) and from 9 healthy controls (median titer 16 IU/ml). Anti-dsDNA depleted polyclonal IgG (anti-dsDNA-dep-IgG) (median anti-dsDNA titer 17 IU/ml) was prepared from sera of these patients with SLE by affinity chromatography with DNA cellulose column. Expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin on human umbilical vein endothelial cells (HUVEC) cultured with either control IgG or anti-dsDNA were compared by how cytometry. The levels of adhesion molecules in the supernatant of cultured HUVEC were assessed by sandwich ELISA. Compared with either control IgG or anti-dsDNA dep-IgG, HUVEC incubated with anti-dsDNA expressed a significantly higher mean fluorescence intensity of ICAM-1 and in VCAM-1 and a higher supernatant concentration of ICAM-1 and VCAM-1 but not E-selectin. At the same time, ICAM-1 mRNA was also raised with increased neutrophil adherence in HUVEC incubated with anti-dsDNA. Pretreatment of HUVEC with native DNA or histone before incubation with anti-dsDNA did not increase the expression of adhesion molecules. Our study provides in vitro evidence that anti-dsDNA could play an important pathogenic role in inducing inflammatory injury of vascular endothelium in SLE.en_HK
dc.languageengen_US
dc.relation.ispartofClinical Immunology and Immunopathologyen_HK
dc.subject.meshAdulten_US
dc.subject.meshAntibodies, Antinuclear - Immunology - Pharmacologyen_US
dc.subject.meshCell Adhesion Molecules - Biosynthesis - Immunologyen_US
dc.subject.meshDna - Immunologyen_US
dc.subject.meshE-Selectin - Biosynthesis - Drug Effects - Geneticsen_US
dc.subject.meshEndothelium, Vascular - Immunology - Metabolismen_US
dc.subject.meshFemaleen_US
dc.subject.meshFlow Cytometryen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoglobulin G - Immunology - Pharmacologyen_US
dc.subject.meshIntercellular Adhesion Molecule-1 - Biosynthesis - Drug Effects - Geneticsen_US
dc.subject.meshLupus Erythematosus, Systemic - Immunologyen_US
dc.subject.meshMaleen_US
dc.subject.meshUmbilical Veinsen_US
dc.subject.meshUp-Regulation - Immunologyen_US
dc.subject.meshVascular Cell Adhesion Molecule-1 - Biosynthesis - Drug Effects - Geneticsen_US
dc.titleUpregulation of adhesion molecule expression on endothelial cells by anti-DNA autoantibodies in systemic lupus erythematosusen_HK
dc.typeArticleen_HK
dc.identifier.emailLai, KN: knlai@hku.hken_HK
dc.identifier.emailLeung, JCK: jckleung@hku.hken_HK
dc.identifier.authorityLai, KN=rp00324en_HK
dc.identifier.authorityLeung, JCK=rp00448en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1006/clin.1996.0183en_HK
dc.identifier.pmid8938099-
dc.identifier.scopuseid_2-s2.0-0030561064en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030561064&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume81en_HK
dc.identifier.issue3en_HK
dc.identifier.spage229en_HK
dc.identifier.epage238en_HK
dc.identifier.isiWOS:A1996VV26800004-
dc.identifier.scopusauthoridLai, KN=7402135706en_HK
dc.identifier.scopusauthoridLeung, JCK=7202180349en_HK
dc.identifier.scopusauthoridLai, KB=7402135525en_HK
dc.identifier.scopusauthoridWong, KC=35104053500en_HK
dc.identifier.scopusauthoridLai, CKW=7403086390en_HK

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