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Article: Transmembrane chloride currents in human atrial myocytes

TitleTransmembrane chloride currents in human atrial myocytes
Authors
Issue Date1996
PublisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpcell.physiology.org/
Citation
American Journal Of Physiology - Cell Physiology, 1996, v. 270 n. 2 39-2, p. C500-C507 How to Cite?
AbstractThe present study was designed to evaluate the presence of basal, swelling-induced, and cAMP-dependent Cl- currents in human atrial myocytes studied with the whole cell patch-clamp technique. Under basal conditions, a small outwardly rectifying background conductance was noted that reversed close to 0 mV and was not altered by Cl- replacement. Isoproterenol (1 μM), forskolin (3 μM), and 8-bromoadenosine 3',5'-cyclic monophosphate (50 μM) did not increase membrane conductance, even when responsiveness to isoproterenol was confirmed by an increase in Ca2+ current and when perforated-patch techniques (nystatin) were used. Exposure to hyposmotic solutions increased cell volume and induced a whole cell conductance that showed outward rectification, was inhibited by 4,4'-diisothiocyanostilbene- 2,2'-disulfonic acid (100 μM), and responded to changes in Cl- gradient in a fashion consistent with a Cl--selective conductance, with estimated relative permeabilities of 1, 0.25, and 0.07 for Cl-, methanesulfonate, and aspartate, respectively. The results suggest that human atrial cells lack basal and adenosine 3',5'-cyclic monophosphate-dependent Cl- current but manifest a substantial Cl- conductance in the presence of cell swelling.
Persistent Identifierhttp://hdl.handle.net/10722/162148
ISSN
2015 Impact Factor: 3.395
2015 SCImago Journal Rankings: 1.893
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, GRen_US
dc.contributor.authorFeng, Jen_US
dc.contributor.authorWang, Zen_US
dc.contributor.authorNattel, Sen_US
dc.date.accessioned2012-09-05T05:17:39Z-
dc.date.available2012-09-05T05:17:39Z-
dc.date.issued1996en_US
dc.identifier.citationAmerican Journal Of Physiology - Cell Physiology, 1996, v. 270 n. 2 39-2, p. C500-C507en_US
dc.identifier.issn0363-6143en_US
dc.identifier.urihttp://hdl.handle.net/10722/162148-
dc.description.abstractThe present study was designed to evaluate the presence of basal, swelling-induced, and cAMP-dependent Cl- currents in human atrial myocytes studied with the whole cell patch-clamp technique. Under basal conditions, a small outwardly rectifying background conductance was noted that reversed close to 0 mV and was not altered by Cl- replacement. Isoproterenol (1 μM), forskolin (3 μM), and 8-bromoadenosine 3',5'-cyclic monophosphate (50 μM) did not increase membrane conductance, even when responsiveness to isoproterenol was confirmed by an increase in Ca2+ current and when perforated-patch techniques (nystatin) were used. Exposure to hyposmotic solutions increased cell volume and induced a whole cell conductance that showed outward rectification, was inhibited by 4,4'-diisothiocyanostilbene- 2,2'-disulfonic acid (100 μM), and responded to changes in Cl- gradient in a fashion consistent with a Cl--selective conductance, with estimated relative permeabilities of 1, 0.25, and 0.07 for Cl-, methanesulfonate, and aspartate, respectively. The results suggest that human atrial cells lack basal and adenosine 3',5'-cyclic monophosphate-dependent Cl- current but manifest a substantial Cl- conductance in the presence of cell swelling.en_US
dc.languageengen_US
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpcell.physiology.org/en_US
dc.relation.ispartofAmerican Journal of Physiology - Cell Physiologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAtrial Functionen_US
dc.subject.meshCell Membrane - Physiologyen_US
dc.subject.meshChlorides - Antagonists & Inhibitors - Physiologyen_US
dc.subject.meshCyclic Amp - Physiologyen_US
dc.subject.meshElectric Conductivityen_US
dc.subject.meshGuinea Pigsen_US
dc.subject.meshHumansen_US
dc.subject.meshMesylates - Pharmacologyen_US
dc.subject.meshMyocardium - Cytologyen_US
dc.subject.meshOsmotic Pressureen_US
dc.titleTransmembrane chloride currents in human atrial myocytesen_US
dc.typeArticleen_US
dc.identifier.emailLi, GR:grli@hkucc.hku.hken_US
dc.identifier.authorityLi, GR=rp00476en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8779912-
dc.identifier.scopuseid_2-s2.0-0029947379en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029947379&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume270en_US
dc.identifier.issue2 39-2en_US
dc.identifier.spageC500en_US
dc.identifier.epageC507en_US
dc.identifier.isiWOS:A1996TV84400012-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLi, GR=7408462932en_US
dc.identifier.scopusauthoridFeng, J=7403884361en_US
dc.identifier.scopusauthoridWang, Z=7410039597en_US
dc.identifier.scopusauthoridNattel, S=36048738800en_US

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