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Article: Anti-DNA autoantibodies stimulate the release of interleukin-1 and interleukin-6 from endothelial cells

TitleAnti-DNA autoantibodies stimulate the release of interleukin-1 and interleukin-6 from endothelial cells
Authors
KeywordsAnti-DNA autoantibodies
Cytokines
Endothelial cells
Issue Date1996
PublisherJohn Wiley & Sons. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/1130
Citation
Journal Of Pathology, 1996, v. 178 n. 4, p. 451-457 How to Cite?
AbstractThe pathogenetic mechanism of vasculitis in systemic lupus erythematosus (SLE) remains a subject of debate. Evidence for a direct pathogenetic role of anti-double-stranded DNA antibodies (anti-dsDNA) is not strong. Supernatant concentrations of interleukin-1β and interleukin-6, and mRNAs encoding for interleukin-1α and interleukin-1 receptor-1 were determined in cultured human umbilical vein endothelial cells (HUVEC), incubated with control IgG (n = 18), anti-dsDNA (n = 18), or IgG from the same lupus patient depleted of anti-dsDNA by affinity chromatography (anti-dsDNA-dep-IgG). Compared with control IgG, there was a significant increase of supernatant interIeukin-1β and interleukin-1α mRNA in endothelial cells incubated with anti-dsDNA. The supernatant interleukin-1β and interleukin-6, and mRNAs encoding for interleukin-1α and interleukin-1 receptor-1, were significantly elevated in endothelial cells incubated with anti-dsDNA, compared with those incubated with anti-dsDNA-dep-IgG. Pretreating HUVEC with native DNA before incubating with anti-dsDNA did not result in an additive effect. These in vitro studies suggest that anti-dsDNA plays an important pathogenetic role in inducing inflammatory injury of vascular endothelium in SLE.
Persistent Identifierhttp://hdl.handle.net/10722/162141
ISSN
2015 Impact Factor: 7.381
2015 SCImago Journal Rankings: 4.176
References

 

DC FieldValueLanguage
dc.contributor.authorLai, KNen_HK
dc.contributor.authorLeung, JCKen_HK
dc.contributor.authorLai, KABen_HK
dc.contributor.authorLi, PKTen_HK
dc.contributor.authorLai, CKWen_HK
dc.date.accessioned2012-09-05T05:17:35Z-
dc.date.available2012-09-05T05:17:35Z-
dc.date.issued1996en_HK
dc.identifier.citationJournal Of Pathology, 1996, v. 178 n. 4, p. 451-457en_HK
dc.identifier.issn0022-3417en_HK
dc.identifier.urihttp://hdl.handle.net/10722/162141-
dc.description.abstractThe pathogenetic mechanism of vasculitis in systemic lupus erythematosus (SLE) remains a subject of debate. Evidence for a direct pathogenetic role of anti-double-stranded DNA antibodies (anti-dsDNA) is not strong. Supernatant concentrations of interleukin-1β and interleukin-6, and mRNAs encoding for interleukin-1α and interleukin-1 receptor-1 were determined in cultured human umbilical vein endothelial cells (HUVEC), incubated with control IgG (n = 18), anti-dsDNA (n = 18), or IgG from the same lupus patient depleted of anti-dsDNA by affinity chromatography (anti-dsDNA-dep-IgG). Compared with control IgG, there was a significant increase of supernatant interIeukin-1β and interleukin-1α mRNA in endothelial cells incubated with anti-dsDNA. The supernatant interleukin-1β and interleukin-6, and mRNAs encoding for interleukin-1α and interleukin-1 receptor-1, were significantly elevated in endothelial cells incubated with anti-dsDNA, compared with those incubated with anti-dsDNA-dep-IgG. Pretreating HUVEC with native DNA before incubating with anti-dsDNA did not result in an additive effect. These in vitro studies suggest that anti-dsDNA plays an important pathogenetic role in inducing inflammatory injury of vascular endothelium in SLE.en_HK
dc.languageengen_US
dc.publisherJohn Wiley & Sons. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/1130en_HK
dc.relation.ispartofJournal of Pathologyen_HK
dc.subjectAnti-DNA autoantibodiesen_HK
dc.subjectCytokinesen_HK
dc.subjectEndothelial cellsen_HK
dc.subject.meshAdulten_US
dc.subject.meshAntibodies, Antinuclear - Immunologyen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCell Culture Techniquesen_US
dc.subject.meshDna - Immunologyen_US
dc.subject.meshEndothelium, Vascular - Immunologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoglobulin G - Immunologyen_US
dc.subject.meshInterleukin-1 - Biosynthesis - Geneticsen_US
dc.subject.meshInterleukin-6 - Biosynthesisen_US
dc.subject.meshLupus Erythematosus, Systemic - Immunologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshRna, Messenger - Geneticsen_US
dc.subject.meshReceptors, Interleukin-1 - Geneticsen_US
dc.titleAnti-DNA autoantibodies stimulate the release of interleukin-1 and interleukin-6 from endothelial cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailLai, KN: knlai@hku.hken_HK
dc.identifier.emailLeung, JCK: jckleung@hku.hken_HK
dc.identifier.authorityLai, KN=rp00324en_HK
dc.identifier.authorityLeung, JCK=rp00448en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/(SICI)1096-9896(199604)178:4<451::AID-PATH499>3.0.CO;2-Pen_HK
dc.identifier.pmid8691326-
dc.identifier.scopuseid_2-s2.0-0029912208en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029912208&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume178en_HK
dc.identifier.issue4en_HK
dc.identifier.spage451en_HK
dc.identifier.epage457en_HK
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLai, KN=7402135706en_HK
dc.identifier.scopusauthoridLeung, JCK=7202180349en_HK
dc.identifier.scopusauthoridLai, KAB=36953972300en_HK
dc.identifier.scopusauthoridLi, PKT=25928016800en_HK
dc.identifier.scopusauthoridLai, CKW=7403086390en_HK

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