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Article: Localisation and expression of β-adrenoceptor subtype mRNAs in human lung

TitleLocalisation and expression of β-adrenoceptor subtype mRNAs in human lung
Authors
Issue Date1996
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ejphar
Citation
European Journal Of Pharmacology, 1996, v. 302 n. 1-3, p. 215-221 How to Cite?
AbstractThe cellular localisation and distribution of mRNAs encoding β- adrenoceptor subtypes in human lung were studied by in situ hybridization and Northern blot analysis. The 851-bp SmaI/PvuII fragment of human β1- adrenoceptor cDNA, the 439-bp SmaI fragment of human β2-adrenoceptor cDNA and the 975-bp SmaI fragment of human β2-adrenoceptor cDNA bound to single mRNA species of approximately 3.2 kb, 2.2 kb and 2.3 kb in size, respectively. Human lung and heart and rabbit lung expressed both β1- and β2-adrenoceptor mRNAs with no detectable level of β3-adrenoceptor mRNA, while rabbit perirenal adipose tissue expressed β1-, β2 and β3- adrenoceptor mRNAs. Cultured human airway epithelial cells and airway smooth muscle cells expressed only β2-adrenoceptor mRNA. In situ hybridization in human lung, using 35S-labelled antisense RNA probes revealed a high level of expression of β1- and β2-adrenoceptor mRNAs in the pulmonary blood vessels, high level of expression of β2-adrenoceptor mRNA in the alveolar walls with less expression of β1-adrenoceptor mRNA. There was a moderate expression of β2-adrenoceptor but not β1-adrenoceptor mRNA in airway epithelium and smooth muscle of peripheral airways and no detectable β3- adrenoceptor mRNA in any lung structures.
Persistent Identifierhttp://hdl.handle.net/10722/162139
ISSN
2015 Impact Factor: 2.73
2015 SCImago Journal Rankings: 1.115
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMak, JCWen_US
dc.contributor.authorNishikawa, Men_US
dc.contributor.authorHaddad, EBen_US
dc.contributor.authorKwon, OJen_US
dc.contributor.authorHirst, SJen_US
dc.contributor.authorTwort, CHCen_US
dc.contributor.authorBarnes, PJen_US
dc.date.accessioned2012-09-05T05:17:35Z-
dc.date.available2012-09-05T05:17:35Z-
dc.date.issued1996en_US
dc.identifier.citationEuropean Journal Of Pharmacology, 1996, v. 302 n. 1-3, p. 215-221en_US
dc.identifier.issn0014-2999en_US
dc.identifier.urihttp://hdl.handle.net/10722/162139-
dc.description.abstractThe cellular localisation and distribution of mRNAs encoding β- adrenoceptor subtypes in human lung were studied by in situ hybridization and Northern blot analysis. The 851-bp SmaI/PvuII fragment of human β1- adrenoceptor cDNA, the 439-bp SmaI fragment of human β2-adrenoceptor cDNA and the 975-bp SmaI fragment of human β2-adrenoceptor cDNA bound to single mRNA species of approximately 3.2 kb, 2.2 kb and 2.3 kb in size, respectively. Human lung and heart and rabbit lung expressed both β1- and β2-adrenoceptor mRNAs with no detectable level of β3-adrenoceptor mRNA, while rabbit perirenal adipose tissue expressed β1-, β2 and β3- adrenoceptor mRNAs. Cultured human airway epithelial cells and airway smooth muscle cells expressed only β2-adrenoceptor mRNA. In situ hybridization in human lung, using 35S-labelled antisense RNA probes revealed a high level of expression of β1- and β2-adrenoceptor mRNAs in the pulmonary blood vessels, high level of expression of β2-adrenoceptor mRNA in the alveolar walls with less expression of β1-adrenoceptor mRNA. There was a moderate expression of β2-adrenoceptor but not β1-adrenoceptor mRNA in airway epithelium and smooth muscle of peripheral airways and no detectable β3- adrenoceptor mRNA in any lung structures.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ejpharen_US
dc.relation.ispartofEuropean Journal of Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBlotting, Northernen_US
dc.subject.meshHumansen_US
dc.subject.meshIn Situ Hybridizationen_US
dc.subject.meshLung - Metabolismen_US
dc.subject.meshMembranes - Metabolismen_US
dc.subject.meshMuscle, Smooth - Drug Effects - Metabolismen_US
dc.subject.meshRna, Messenger - Genetics - Metabolismen_US
dc.subject.meshRabbitsen_US
dc.subject.meshReceptors, Adrenergic, Beta - Classification - Genetics - Metabolismen_US
dc.titleLocalisation and expression of β-adrenoceptor subtype mRNAs in human lungen_US
dc.typeArticleen_US
dc.identifier.emailMak, JCW:judymak@hku.hken_US
dc.identifier.authorityMak, JCW=rp00352en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0014-2999(96)00104-5en_US
dc.identifier.pmid8791010-
dc.identifier.scopuseid_2-s2.0-0029904177en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029904177&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume302en_US
dc.identifier.issue1-3en_US
dc.identifier.spage215en_US
dc.identifier.epage221en_US
dc.identifier.isiWOS:A1996UQ81700027-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridMak, JCW=7103323094en_US
dc.identifier.scopusauthoridNishikawa, M=7402607361en_US
dc.identifier.scopusauthoridHaddad, EB=7102803008en_US
dc.identifier.scopusauthoridKwon, OJ=7402194121en_US
dc.identifier.scopusauthoridHirst, SJ=7006389991en_US
dc.identifier.scopusauthoridTwort, CHC=6701446858en_US
dc.identifier.scopusauthoridBarnes, PJ=36064679400en_US

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