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Article: Properties of sodium and potassium currents of cultured adult human atrial myocytes

TitleProperties of sodium and potassium currents of cultured adult human atrial myocytes
Authors
Issue Date1996
PublisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpheart.physiology.org/
Citation
American Journal Of Physiology - Heart And Circulatory Physiology, 1996, v. 270 n. 5 39-5, p. H1676-H1686 How to Cite?
AbstractCultured cell systems are valuable for the study of regulation of phenotypic expression, but little is known about the electrophysiological properties of human cardiac tissues in culture. The present studies were designed to determine the feasibility of maintaining human atrial myocytes in primary culture and to assess changes in Na+ (I(Na)) and K+ (I(to), transient outward, and I(Kur), ultra-rapid delayed rectifier) currents. Within 24 h of culture, cells assumed an ovoid shape, which they maintained for up to 7 days. The voltage dependence, kinetics, and density of I(Na) were unchanged in culture. The activation properties of I(to) (kinetics and voltage dependence) were not altered, but I(to) density (current normalized to cell capacitance) was reduced and inactivation properties were altered (negative shift in voltage dependence and slowed kinetics) in cultured compared with fresh cells. The absolute current amplitude, kinetics, voltage dependence, and 4-aminopyridine sensitivity of I(Kur) were unchanged, but current density was increased. All changes in ionic currents occurred within 24 h of culture and remained stable for the next 4 days. We conclude that human atrial myocytes can be maintained in primary culture, that the qualitative properties of I(Na), I(to), and I(Kur) remain constant but that some quantitative changes occur, and that cultured human atrial myocytes may be valuable for studies of the molecular mechanisms and regulation of cardiac ion channel function in humans.
Persistent Identifierhttp://hdl.handle.net/10722/162136
ISSN
2015 Impact Factor: 3.324
2015 SCImago Journal Rankings: 1.823
References

 

DC FieldValueLanguage
dc.contributor.authorFeng, Jen_US
dc.contributor.authorLi, GRen_US
dc.contributor.authorFermini, Ben_US
dc.contributor.authorNattel, Sen_US
dc.date.accessioned2012-09-05T05:17:34Z-
dc.date.available2012-09-05T05:17:34Z-
dc.date.issued1996en_US
dc.identifier.citationAmerican Journal Of Physiology - Heart And Circulatory Physiology, 1996, v. 270 n. 5 39-5, p. H1676-H1686en_US
dc.identifier.issn0363-6135en_US
dc.identifier.urihttp://hdl.handle.net/10722/162136-
dc.description.abstractCultured cell systems are valuable for the study of regulation of phenotypic expression, but little is known about the electrophysiological properties of human cardiac tissues in culture. The present studies were designed to determine the feasibility of maintaining human atrial myocytes in primary culture and to assess changes in Na+ (I(Na)) and K+ (I(to), transient outward, and I(Kur), ultra-rapid delayed rectifier) currents. Within 24 h of culture, cells assumed an ovoid shape, which they maintained for up to 7 days. The voltage dependence, kinetics, and density of I(Na) were unchanged in culture. The activation properties of I(to) (kinetics and voltage dependence) were not altered, but I(to) density (current normalized to cell capacitance) was reduced and inactivation properties were altered (negative shift in voltage dependence and slowed kinetics) in cultured compared with fresh cells. The absolute current amplitude, kinetics, voltage dependence, and 4-aminopyridine sensitivity of I(Kur) were unchanged, but current density was increased. All changes in ionic currents occurred within 24 h of culture and remained stable for the next 4 days. We conclude that human atrial myocytes can be maintained in primary culture, that the qualitative properties of I(Na), I(to), and I(Kur) remain constant but that some quantitative changes occur, and that cultured human atrial myocytes may be valuable for studies of the molecular mechanisms and regulation of cardiac ion channel function in humans.en_US
dc.languageengen_US
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpheart.physiology.org/en_US
dc.relation.ispartofAmerican Journal of Physiology - Heart and Circulatory Physiologyen_US
dc.subject.mesh4-Aminopyridine - Pharmacologyen_US
dc.subject.meshAgeden_US
dc.subject.meshAtrial Functionen_US
dc.subject.meshCalcium - Physiologyen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshElectric Conductivityen_US
dc.subject.meshFeasibility Studiesen_US
dc.subject.meshHumansen_US
dc.subject.meshKineticsen_US
dc.subject.meshMembrane Potentialsen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshMyocardium - Cytologyen_US
dc.subject.meshPotassium - Physiologyen_US
dc.subject.meshSodium - Physiologyen_US
dc.titleProperties of sodium and potassium currents of cultured adult human atrial myocytesen_US
dc.typeArticleen_US
dc.identifier.emailLi, GR:grli@hkucc.hku.hken_US
dc.identifier.authorityLi, GR=rp00476en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8928874-
dc.identifier.scopuseid_2-s2.0-0029889404en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029889404&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume270en_US
dc.identifier.issue5 39-5en_US
dc.identifier.spageH1676en_US
dc.identifier.epageH1686en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridFeng, J=7403884361en_US
dc.identifier.scopusauthoridLi, GR=7408462932en_US
dc.identifier.scopusauthoridFermini, B=6603996429en_US
dc.identifier.scopusauthoridNattel, S=36048738800en_US

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