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Article: Directed mutagenesis of the Rhodobacter capsulatus puhA gene and Orf 214: Pleiotropic effects on photosynthetic reaction center and light-harvesting 1 complexes

TitleDirected mutagenesis of the Rhodobacter capsulatus puhA gene and Orf 214: Pleiotropic effects on photosynthetic reaction center and light-harvesting 1 complexes
Authors
Issue Date1996
Citation
Journal Of Bacteriology, 1996, v. 178 n. 8, p. 2334-2342 How to Cite?
AbstractRhodobacter capsulatus puhA mutant strains containing either a nonpolar, translationally in-frame deletion or a polar insertion of an antibiotic resistance cartridge were constructed and evaluated for their photosynthetic growth properties, absorption spectroscopy profiles, and chromatophore protein compositions. Both types of mutants were found to be incapable of photosynthetic growth and deficient in the reaction center (RC) and light- harvesting 1 (LH1) complexes. The translationally in-frame puhA deletion strains were restored to the parental strain phenotypes by complementation with a plasmid containing the puhA gene, whereas the polar puhA mutants were not. Analogous nonpolar and polar disruptions of orf 214 (located immediately 3' of the puhA gene) were made, and the resultant mutant strains were evaluated as described above. The strain containing the nonpolar deletion of orf 214 exhibited severely impaired photosynthetic growth properties and had greatly reduced levels of the RC and LH1 complexes. Complementation of this strain with a plasmid that expressed off 214 from the nifHDK promoter restored photosynthetic growth capability, as well as the RC and LH1 complexes. The polar disruption of orf 214 yielded cells that were incapable of photosynthetic growth and had even lower levels of the RC and LH1 complexes, and complementation in trans with orf 214 only marginally improved these deficiencies. These results indicate that off 214 and at least one additional gene located 3' of orf 214 are required to obtain the RC and LH1 complexes, and transcription read-through from the puhA superoperon is necessary for optimal expression of these new photosynthesis genes.
Persistent Identifierhttp://hdl.handle.net/10722/162135
ISSN
2015 Impact Factor: 3.198
2015 SCImago Journal Rankings: 2.216
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWong, DKHen_US
dc.contributor.authorCollins, WJen_US
dc.contributor.authorHarmer, Aen_US
dc.contributor.authorLilburn, TGen_US
dc.contributor.authorBeatty, JTen_US
dc.date.accessioned2012-09-05T05:17:34Z-
dc.date.available2012-09-05T05:17:34Z-
dc.date.issued1996en_US
dc.identifier.citationJournal Of Bacteriology, 1996, v. 178 n. 8, p. 2334-2342en_US
dc.identifier.issn0021-9193en_US
dc.identifier.urihttp://hdl.handle.net/10722/162135-
dc.description.abstractRhodobacter capsulatus puhA mutant strains containing either a nonpolar, translationally in-frame deletion or a polar insertion of an antibiotic resistance cartridge were constructed and evaluated for their photosynthetic growth properties, absorption spectroscopy profiles, and chromatophore protein compositions. Both types of mutants were found to be incapable of photosynthetic growth and deficient in the reaction center (RC) and light- harvesting 1 (LH1) complexes. The translationally in-frame puhA deletion strains were restored to the parental strain phenotypes by complementation with a plasmid containing the puhA gene, whereas the polar puhA mutants were not. Analogous nonpolar and polar disruptions of orf 214 (located immediately 3' of the puhA gene) were made, and the resultant mutant strains were evaluated as described above. The strain containing the nonpolar deletion of orf 214 exhibited severely impaired photosynthetic growth properties and had greatly reduced levels of the RC and LH1 complexes. Complementation of this strain with a plasmid that expressed off 214 from the nifHDK promoter restored photosynthetic growth capability, as well as the RC and LH1 complexes. The polar disruption of orf 214 yielded cells that were incapable of photosynthetic growth and had even lower levels of the RC and LH1 complexes, and complementation in trans with orf 214 only marginally improved these deficiencies. These results indicate that off 214 and at least one additional gene located 3' of orf 214 are required to obtain the RC and LH1 complexes, and transcription read-through from the puhA superoperon is necessary for optimal expression of these new photosynthesis genes.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Bacteriologyen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshBacterial Proteinsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshGenes, Bacterialen_US
dc.subject.meshLac Operonen_US
dc.subject.meshLight-Harvesting Protein Complexesen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMutagenesis, Insertionalen_US
dc.subject.meshMutagenesis, Site-Directeden_US
dc.subject.meshOpen Reading Framesen_US
dc.subject.meshPhotosynthesis - Geneticsen_US
dc.subject.meshPhotosynthetic Reaction Center Complex Proteins - Geneticsen_US
dc.subject.meshRhodobacter Capsulatus - Genetics - Growth & Development - Metabolismen_US
dc.titleDirected mutagenesis of the Rhodobacter capsulatus puhA gene and Orf 214: Pleiotropic effects on photosynthetic reaction center and light-harvesting 1 complexesen_US
dc.typeArticleen_US
dc.identifier.emailWong, DKH:danywong@hku.hken_US
dc.identifier.authorityWong, DKH=rp00492en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8636035-
dc.identifier.scopuseid_2-s2.0-0029885004en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029885004&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume178en_US
dc.identifier.issue8en_US
dc.identifier.spage2334en_US
dc.identifier.epage2342en_US
dc.identifier.isiWOS:A1996UF03600022-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWong, DKH=7401535819en_US
dc.identifier.scopusauthoridCollins, WJ=16746086000en_US
dc.identifier.scopusauthoridHarmer, A=7003687954en_US
dc.identifier.scopusauthoridLilburn, TG=6602880137en_US
dc.identifier.scopusauthoridBeatty, JT=7103289658en_US

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