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Article: IL-1β, a major stimulator of hyaluronan synthesis in vitro of human peritoneal mesothelial cells: Relevance to peritonitis in CAPD

TitleIL-1β, a major stimulator of hyaluronan synthesis in vitro of human peritoneal mesothelial cells: Relevance to peritonitis in CAPD
Authors
Issue Date1996
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/ki/index.html
Citation
Kidney International, 1996, v. 50 n. 4, p. 1337-1343 How to Cite?
AbstractThe effect of several different growth factors and cytokines on the synthesis of hyaluronan (HA) by human peritoneal mesothelial cells (HPMC) was investigated. Growth arrested HPMC synthesized low levels of HA, but co-culture with PDGF-bb, TGF-β1, TNF-α, and IL-6 at a concentration of 10 ng/ml all increased HA synthesis between two- to three-fold. At the same concentration IL-1β significantly increased the synthesis eight-fold (N = 3; P < 0.05). The effect of IL-1β was also dose- and time-dependent and could be totally negated with interleukin-1 receptor antagonist (IL-1β RcA). Non-infected and infected dialysate from patients receiving CAPD was also found to stimulate PIA synthesis by HPMC. The levels found with non-infected fluid were 4 x 10 4 dpm/ml (N = 6) and 12.9 x 10 4 dpm/ml (N = 6; P < 0.002) and 8.7 x 10 4 dpm/ml (N = 6; P < 0.003) for infected fluid collected one and two days after the commencement of peritonitis. IL-1β RcA dramatically reduced the effect of infected but not noninfected dialysate. These results provide new insights into the manner in which HA synthesis is controlled in the mesothelium and suggest that IL-1β is a key cytokine in the inflammatory response in CAPD patients.
Persistent Identifierhttp://hdl.handle.net/10722/162128
ISSN
2015 Impact Factor: 7.683
2015 SCImago Journal Rankings: 3.181
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYung, Sen_US
dc.contributor.authorColes, GAen_US
dc.contributor.authorDavies, Men_US
dc.date.accessioned2012-09-05T05:17:30Z-
dc.date.available2012-09-05T05:17:30Z-
dc.date.issued1996en_US
dc.identifier.citationKidney International, 1996, v. 50 n. 4, p. 1337-1343en_US
dc.identifier.issn0085-2538en_US
dc.identifier.urihttp://hdl.handle.net/10722/162128-
dc.description.abstractThe effect of several different growth factors and cytokines on the synthesis of hyaluronan (HA) by human peritoneal mesothelial cells (HPMC) was investigated. Growth arrested HPMC synthesized low levels of HA, but co-culture with PDGF-bb, TGF-β1, TNF-α, and IL-6 at a concentration of 10 ng/ml all increased HA synthesis between two- to three-fold. At the same concentration IL-1β significantly increased the synthesis eight-fold (N = 3; P < 0.05). The effect of IL-1β was also dose- and time-dependent and could be totally negated with interleukin-1 receptor antagonist (IL-1β RcA). Non-infected and infected dialysate from patients receiving CAPD was also found to stimulate PIA synthesis by HPMC. The levels found with non-infected fluid were 4 x 10 4 dpm/ml (N = 6) and 12.9 x 10 4 dpm/ml (N = 6; P < 0.002) and 8.7 x 10 4 dpm/ml (N = 6; P < 0.003) for infected fluid collected one and two days after the commencement of peritonitis. IL-1β RcA dramatically reduced the effect of infected but not noninfected dialysate. These results provide new insights into the manner in which HA synthesis is controlled in the mesothelium and suggest that IL-1β is a key cytokine in the inflammatory response in CAPD patients.en_US
dc.languageengen_US
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/ki/index.htmlen_US
dc.relation.ispartofKidney Internationalen_US
dc.subject.meshAdolescenten_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAged, 80 And Overen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCytokines - Pharmacologyen_US
dc.subject.meshDialysis Solutions - Pharmacologyen_US
dc.subject.meshEpithelial Cellsen_US
dc.subject.meshEpithelium - Drug Effectsen_US
dc.subject.meshHemoglobinsen_US
dc.subject.meshHumansen_US
dc.subject.meshHyaluronic Acid - Biosynthesis - Chemistryen_US
dc.subject.meshInterleukin-1 - Pharmacologyen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshPeritoneal Cavity - Cytologyen_US
dc.subject.meshPeritoneal Dialysis, Continuous Ambulatoryen_US
dc.subject.meshPeritonitis - Metabolismen_US
dc.titleIL-1β, a major stimulator of hyaluronan synthesis in vitro of human peritoneal mesothelial cells: Relevance to peritonitis in CAPDen_US
dc.typeArticleen_US
dc.identifier.emailYung, S:ssyyung@hku.hken_US
dc.identifier.authorityYung, S=rp00455en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1038/ki.1996.446-
dc.identifier.pmid8887296-
dc.identifier.scopuseid_2-s2.0-0029828628en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029828628&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume50en_US
dc.identifier.issue4en_US
dc.identifier.spage1337en_US
dc.identifier.epage1343en_US
dc.identifier.isiWOS:A1996VJ31400033-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridYung, S=22636568800en_US
dc.identifier.scopusauthoridColes, GA=7102488081en_US
dc.identifier.scopusauthoridDavies, M=7404207291en_US

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