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Article: Muscarinic and β-adrenergic receptor expression in peripheral lung from normal and asthmatic patients

TitleMuscarinic and β-adrenergic receptor expression in peripheral lung from normal and asthmatic patients
Authors
Issue Date1996
PublisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajplung.physiology.org/
Citation
American Journal Of Physiology - Lung Cellular And Molecular Physiology, 1996, v. 270 n. 6 14-6, p. L947-L953 How to Cite?
AbstractWe used human peripheral lung from 8 mildly asthmatic patients and 11 normal donors to study the expression of muscarinic and β-adrenergic receptors in asthma. There was no significant difference in the affinity or the density of muscarinic (labeled with [N-methyl-3H]scopolamine) and β1- and β2-adrenergic receptors (labeled with [125I]iodocyanopindolol) in peripheral lung from asthmatics compared with nonasthmatics. Only the muscarinic m1 receptor mRNA was detected in human lung using Northern blot analysis. Additionally, peripheral lung cellular mRNA hybridized to human β1 and β2 cDNA probes, giving 3.2- and 2.2-kb bands corresponding to β1 and β2-adrenergic receptors mRNA, respectively. Densitometric scanning of the autoradiograms suggests that there was no significant difference in the relative abundance of muscarinic m1 and β1- and β2-adrenergic receptor mRNA in asthmatic compared with nonasthmatic lungs. Functional experiments obtained in trachea suggest that there was an increase in the cholinergic neural response evoked by electrical field stimulation in asthmatic compared with nonasthmatic tissues which was not due to a reduction in inhibitory noncholinergic nonadrenergic relaxations.
Persistent Identifierhttp://hdl.handle.net/10722/162123
ISSN
2015 Impact Factor: 4.721
2015 SCImago Journal Rankings: 1.838
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHaddad, EBen_US
dc.contributor.authorMak, JCWen_US
dc.contributor.authorBelvisi, MGen_US
dc.contributor.authorNishikawa, Men_US
dc.contributor.authorRousell, Jen_US
dc.contributor.authorBarnes, PJen_US
dc.date.accessioned2012-09-05T05:17:28Z-
dc.date.available2012-09-05T05:17:28Z-
dc.date.issued1996en_US
dc.identifier.citationAmerican Journal Of Physiology - Lung Cellular And Molecular Physiology, 1996, v. 270 n. 6 14-6, p. L947-L953en_US
dc.identifier.issn1040-0605en_US
dc.identifier.urihttp://hdl.handle.net/10722/162123-
dc.description.abstractWe used human peripheral lung from 8 mildly asthmatic patients and 11 normal donors to study the expression of muscarinic and β-adrenergic receptors in asthma. There was no significant difference in the affinity or the density of muscarinic (labeled with [N-methyl-3H]scopolamine) and β1- and β2-adrenergic receptors (labeled with [125I]iodocyanopindolol) in peripheral lung from asthmatics compared with nonasthmatics. Only the muscarinic m1 receptor mRNA was detected in human lung using Northern blot analysis. Additionally, peripheral lung cellular mRNA hybridized to human β1 and β2 cDNA probes, giving 3.2- and 2.2-kb bands corresponding to β1 and β2-adrenergic receptors mRNA, respectively. Densitometric scanning of the autoradiograms suggests that there was no significant difference in the relative abundance of muscarinic m1 and β1- and β2-adrenergic receptor mRNA in asthmatic compared with nonasthmatic lungs. Functional experiments obtained in trachea suggest that there was an increase in the cholinergic neural response evoked by electrical field stimulation in asthmatic compared with nonasthmatic tissues which was not due to a reduction in inhibitory noncholinergic nonadrenergic relaxations.en_US
dc.languageengen_US
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajplung.physiology.org/en_US
dc.relation.ispartofAmerican Journal of Physiology - Lung Cellular and Molecular Physiologyen_US
dc.subject.meshAdulten_US
dc.subject.meshAsthma - Metabolism - Physiopathologyen_US
dc.subject.meshBlotting, Northernen_US
dc.subject.meshElectric Stimulationen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshLung - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshParasympathetic Nervous System - Physiopathologyen_US
dc.subject.meshRna, Messenger - Metabolismen_US
dc.subject.meshRadioligand Assayen_US
dc.subject.meshReceptors, Adrenergic, Beta - Genetics - Metabolismen_US
dc.subject.meshReceptors, Muscarinic - Genetics - Metabolismen_US
dc.subject.meshReference Valuesen_US
dc.subject.meshTrachea - Innervationen_US
dc.titleMuscarinic and β-adrenergic receptor expression in peripheral lung from normal and asthmatic patientsen_US
dc.typeArticleen_US
dc.identifier.emailMak, JCW:judymak@hku.hken_US
dc.identifier.authorityMak, JCW=rp00352en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8764219-
dc.identifier.scopuseid_2-s2.0-0029775155en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029775155&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume270en_US
dc.identifier.issue6 14-6en_US
dc.identifier.spageL947en_US
dc.identifier.epageL953en_US
dc.identifier.isiWOS:A1996UT99800008-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridHaddad, EB=7102803008en_US
dc.identifier.scopusauthoridMak, JCW=7103323094en_US
dc.identifier.scopusauthoridBelvisi, MG=35400532900en_US
dc.identifier.scopusauthoridNishikawa, M=7402607361en_US
dc.identifier.scopusauthoridRousell, J=6602560061en_US
dc.identifier.scopusauthoridBarnes, PJ=36064679400en_US

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