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Article: Mixed chimerism following bone marrow transplantation for severe combined immunodeficiency: A study by DNA fingerprinting and simultaneous immunophenotyping and fluorescence in situ hybridisation

TitleMixed chimerism following bone marrow transplantation for severe combined immunodeficiency: A study by DNA fingerprinting and simultaneous immunophenotyping and fluorescence in situ hybridisation
Authors
Issue Date1995
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/bmt
Citation
Bone Marrow Transplantation, 1995, v. 15 n. 6, p. 971-976 How to Cite?
AbstractWe report a girl with severe combined immunodeficiency (SCID) who had a paternal T-depleted bone marrow transplant (BMT) when 11 months old. Engraftment was documented but karyotyping of marrow cells 1 year after BMT showed recipient metaphases (XX) only. However, she remained clinically well and further analysis by karyotyping of PHA-cultured peripheral blood mononuclear cells (PBMC) showed donor metaphases (XY) only. DNA fingerprinting confirmed mixed chimerism in the peripheral blood. The granulocytes were of recipient origin and the PBMC of mixed origin, the donor proportion of which increased after culture with PHA. Using simultaneous immunophenotyping and fluorescence in situ hybridisation (FISH) with chromosomes X and Y-specific probes, circulating T cells were demonstrated to be of donor origin whereas B cells and myeloid cells were mostly of recipient origin.
Persistent Identifierhttp://hdl.handle.net/10722/162087
ISSN
2015 Impact Factor: 3.636
2015 SCImago Journal Rankings: 1.585
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLau, YLen_US
dc.contributor.authorKwong, YLen_US
dc.contributor.authorLee, ACWen_US
dc.contributor.authorChiu, EKWen_US
dc.contributor.authorHa, SYen_US
dc.contributor.authorChan, CFen_US
dc.contributor.authorChan, Ven_US
dc.contributor.authorChan, TKen_US
dc.date.accessioned2012-09-05T05:17:12Z-
dc.date.available2012-09-05T05:17:12Z-
dc.date.issued1995en_US
dc.identifier.citationBone Marrow Transplantation, 1995, v. 15 n. 6, p. 971-976en_US
dc.identifier.issn0268-3369en_US
dc.identifier.urihttp://hdl.handle.net/10722/162087-
dc.description.abstractWe report a girl with severe combined immunodeficiency (SCID) who had a paternal T-depleted bone marrow transplant (BMT) when 11 months old. Engraftment was documented but karyotyping of marrow cells 1 year after BMT showed recipient metaphases (XX) only. However, she remained clinically well and further analysis by karyotyping of PHA-cultured peripheral blood mononuclear cells (PBMC) showed donor metaphases (XY) only. DNA fingerprinting confirmed mixed chimerism in the peripheral blood. The granulocytes were of recipient origin and the PBMC of mixed origin, the donor proportion of which increased after culture with PHA. Using simultaneous immunophenotyping and fluorescence in situ hybridisation (FISH) with chromosomes X and Y-specific probes, circulating T cells were demonstrated to be of donor origin whereas B cells and myeloid cells were mostly of recipient origin.en_US
dc.languageengen_US
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/bmten_US
dc.relation.ispartofBone Marrow Transplantationen_US
dc.subject.meshAdulten_US
dc.subject.meshBiological Markersen_US
dc.subject.meshBone Marrow Examination - Methodsen_US
dc.subject.meshBone Marrow Transplantation - Pathologyen_US
dc.subject.meshChimeraen_US
dc.subject.meshDna Fingerprintingen_US
dc.subject.meshFemaleen_US
dc.subject.meshGraft Survivalen_US
dc.subject.meshGranulocytesen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunophenotypingen_US
dc.subject.meshIn Situ Hybridization, Fluorescenceen_US
dc.subject.meshInfanten_US
dc.subject.meshLeukocytes, Mononuclearen_US
dc.subject.meshLymphocyte Depletionen_US
dc.subject.meshMaleen_US
dc.subject.meshSevere Combined Immunodeficiency - Pathology - Therapyen_US
dc.subject.meshT-Lymphocytesen_US
dc.subject.meshX Chromosomeen_US
dc.subject.meshY Chromosomeen_US
dc.titleMixed chimerism following bone marrow transplantation for severe combined immunodeficiency: A study by DNA fingerprinting and simultaneous immunophenotyping and fluorescence in situ hybridisationen_US
dc.typeArticleen_US
dc.identifier.emailLau, YL:lauylung@hkucc.hku.hken_US
dc.identifier.emailKwong, YL:ylkwong@hku.hken_US
dc.identifier.emailChan, V:vnychana@hkucc.hku.hken_US
dc.identifier.authorityLau, YL=rp00361en_US
dc.identifier.authorityKwong, YL=rp00358en_US
dc.identifier.authorityChan, V=rp00320en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid7581099-
dc.identifier.scopuseid_2-s2.0-0029026407en_US
dc.identifier.hkuros12306-
dc.identifier.hkuros8435-
dc.identifier.volume15en_US
dc.identifier.issue6en_US
dc.identifier.spage971en_US
dc.identifier.epage976en_US
dc.identifier.isiWOS:A1995RJ19600025-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridLau, YL=7201403380en_US
dc.identifier.scopusauthoridKwong, YL=7102818954en_US
dc.identifier.scopusauthoridLee, ACW=7405631431en_US
dc.identifier.scopusauthoridChiu, EKW=24827833600en_US
dc.identifier.scopusauthoridHa, SY=7202501115en_US
dc.identifier.scopusauthoridChan, CF=36984590800en_US
dc.identifier.scopusauthoridChan, V=7202654865en_US
dc.identifier.scopusauthoridChan, TK=7402687762en_US

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