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Article: Source of peritoneal proteoglycans: Human peritoneal mesothelial cells synthesize and secrete mainly small dermatan sulfate proteoglycans

TitleSource of peritoneal proteoglycans: Human peritoneal mesothelial cells synthesize and secrete mainly small dermatan sulfate proteoglycans
Authors
Issue Date1995
PublisherAmerican Society for Investigative Pathology. The Journal's web site is located at http://www.amjpathol.org
Citation
American Journal Of Pathology, 1995, v. 146 n. 2, p. 520-529 How to Cite?
AbstractThis study describes experiments that compare the proteoglycans (PGs) extracted from the dialysate from patients receiving continuous peritoneal ambulatory dialysis (CAPD) with those secreted by metabolically labeled human peritoneal mesothelial cells in vitro. The PGs isolated from both sources were predominantly small chondroitin sulfate/dermatan sulfate PGs. Western blot of the core proteins obtained after chondroitin ABC lyase treatment with specific antibodies identified decorin and biglycan. With [ 35S]sulfate and [ 35S]methionine as labeling precursors it was shown that dermatan sulfate rather than chondroitin sulfate were the major glycosaminoglycan chains and that decorin was the predominant species. These data provide the first evidence that human peritoneal mesothelial cells may be the principal source of PGs in the peritoneum. Given the proposed functions of decorin and biglycan, the results suggest that these PGs may be involved in the control of transforming growth factor-β activity and collagen fibril formation in the peritoneum.
Persistent Identifierhttp://hdl.handle.net/10722/162072
ISSN
2015 Impact Factor: 4.206
2015 SCImago Journal Rankings: 2.653
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYung, Sen_US
dc.contributor.authorThomas, GJen_US
dc.contributor.authorStylianou, Een_US
dc.contributor.authorWilliams, JDen_US
dc.contributor.authorColes, GAen_US
dc.contributor.authorDavies, Men_US
dc.date.accessioned2012-09-05T05:17:04Z-
dc.date.available2012-09-05T05:17:04Z-
dc.date.issued1995en_US
dc.identifier.citationAmerican Journal Of Pathology, 1995, v. 146 n. 2, p. 520-529en_US
dc.identifier.issn0002-9440en_US
dc.identifier.urihttp://hdl.handle.net/10722/162072-
dc.description.abstractThis study describes experiments that compare the proteoglycans (PGs) extracted from the dialysate from patients receiving continuous peritoneal ambulatory dialysis (CAPD) with those secreted by metabolically labeled human peritoneal mesothelial cells in vitro. The PGs isolated from both sources were predominantly small chondroitin sulfate/dermatan sulfate PGs. Western blot of the core proteins obtained after chondroitin ABC lyase treatment with specific antibodies identified decorin and biglycan. With [ 35S]sulfate and [ 35S]methionine as labeling precursors it was shown that dermatan sulfate rather than chondroitin sulfate were the major glycosaminoglycan chains and that decorin was the predominant species. These data provide the first evidence that human peritoneal mesothelial cells may be the principal source of PGs in the peritoneum. Given the proposed functions of decorin and biglycan, the results suggest that these PGs may be involved in the control of transforming growth factor-β activity and collagen fibril formation in the peritoneum.en_US
dc.languageengen_US
dc.publisherAmerican Society for Investigative Pathology. The Journal's web site is located at http://www.amjpathol.orgen_US
dc.relation.ispartofAmerican Journal of Pathologyen_US
dc.subject.meshAscitic Fluid - Chemistryen_US
dc.subject.meshBiglycanen_US
dc.subject.meshChondroitin Sulfates - Chemistry - Isolation & Purification - Metabolismen_US
dc.subject.meshDecorinen_US
dc.subject.meshDermatan Sulfate - Isolation & Purification - Metabolismen_US
dc.subject.meshEpithelium - Metabolismen_US
dc.subject.meshExtracellular Matrix Proteinsen_US
dc.subject.meshHumansen_US
dc.subject.meshKidney Failure, Chronic - Therapyen_US
dc.subject.meshPeritoneal Dialysis, Continuous Ambulatoryen_US
dc.subject.meshPeritoneum - Metabolismen_US
dc.subject.meshProteoglycans - Chemistry - Isolation & Purification - Metabolismen_US
dc.titleSource of peritoneal proteoglycans: Human peritoneal mesothelial cells synthesize and secrete mainly small dermatan sulfate proteoglycansen_US
dc.typeArticleen_US
dc.identifier.emailYung, S:ssyyung@hku.hken_US
dc.identifier.authorityYung, S=rp00455en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid7856761en_US
dc.identifier.scopuseid_2-s2.0-0028878579en_US
dc.identifier.volume146en_US
dc.identifier.issue2en_US
dc.identifier.spage520en_US
dc.identifier.epage529en_US
dc.identifier.isiWOS:A1995QF42700024-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridYung, S=22636568800en_US
dc.identifier.scopusauthoridThomas, GJ=35464458200en_US
dc.identifier.scopusauthoridStylianou, E=6701765918en_US
dc.identifier.scopusauthoridWilliams, JD=35600499200en_US
dc.identifier.scopusauthoridColes, GA=7102488081en_US
dc.identifier.scopusauthoridDavies, M=7404207291en_US

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