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Article: Use of the polymerase chain reaction in the detection of AML1/ETO fusion transcript in t(8;21)

TitleUse of the polymerase chain reaction in the detection of AML1/ETO fusion transcript in t(8;21)
Authors
Issue Date1995
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/28741
Citation
Cancer, 1995, v. 75 n. 3, p. 821-825 How to Cite?
AbstractBackground. t(8;21)(q22;q22), found in acute myeloid leukemia (AML) and occasionally in myelodysplasia (MDS), results in the fusion of the AML1 gene on 22q22 to the ETO gene on 8q22, generating a chimeric AML1/ETO transcript, which is a molecular marker of the translocation. Methods. Reverse transcription-polymerase chain reaction (RT-PCR), with two pairs of nested AML1 and ETO primers, was used to amplify the AML1/ETO fusion transcript. The Kasumi-1 cell line was used as a positive control. Results. RT-PCR has a sensitivity of 0.0001% (10 -6), corresponding to detection of 0.5 picograms of leukemic RNA in the presence of 0.5 μg of normal RNA. Using this approach, patients with t(8;21) (three patients with de novo AML, one with therapy-related AML, and one patient with myelodysplasia) yielded the same 222 base pair PCR product, suggesting that the breakpoints occurred at the same AML1 and ETO introns as previously reported. Three patients were still PCR-positive when in complete remission after chemotherapy and two experienced relapse. However, in another three patients with t(8;21) who were in remission for 2 months, 2 years, and 3 1/2 years, respectively, PCR was negative. Conclusion. RT-PCR is a sensitive method of detection of t(8;21), and is useful in the monitoring of minimal residual leukemia. As the junction of AML1/ETO appears to be constant, RT-PCR may offer a quick and accurate diagnosis of t(8;21).
Persistent Identifierhttp://hdl.handle.net/10722/162071
ISSN
2015 Impact Factor: 5.649
2015 SCImago Journal Rankings: 3.188
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorKwong, YLen_US
dc.contributor.authorChan, Ven_US
dc.contributor.authorWong, KFen_US
dc.contributor.authorChan, TKen_US
dc.date.accessioned2012-09-05T05:17:04Z-
dc.date.available2012-09-05T05:17:04Z-
dc.date.issued1995en_US
dc.identifier.citationCancer, 1995, v. 75 n. 3, p. 821-825en_US
dc.identifier.issn0008-543Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/162071-
dc.description.abstractBackground. t(8;21)(q22;q22), found in acute myeloid leukemia (AML) and occasionally in myelodysplasia (MDS), results in the fusion of the AML1 gene on 22q22 to the ETO gene on 8q22, generating a chimeric AML1/ETO transcript, which is a molecular marker of the translocation. Methods. Reverse transcription-polymerase chain reaction (RT-PCR), with two pairs of nested AML1 and ETO primers, was used to amplify the AML1/ETO fusion transcript. The Kasumi-1 cell line was used as a positive control. Results. RT-PCR has a sensitivity of 0.0001% (10 -6), corresponding to detection of 0.5 picograms of leukemic RNA in the presence of 0.5 μg of normal RNA. Using this approach, patients with t(8;21) (three patients with de novo AML, one with therapy-related AML, and one patient with myelodysplasia) yielded the same 222 base pair PCR product, suggesting that the breakpoints occurred at the same AML1 and ETO introns as previously reported. Three patients were still PCR-positive when in complete remission after chemotherapy and two experienced relapse. However, in another three patients with t(8;21) who were in remission for 2 months, 2 years, and 3 1/2 years, respectively, PCR was negative. Conclusion. RT-PCR is a sensitive method of detection of t(8;21), and is useful in the monitoring of minimal residual leukemia. As the junction of AML1/ETO appears to be constant, RT-PCR may offer a quick and accurate diagnosis of t(8;21).en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/28741en_US
dc.relation.ispartofCanceren_US
dc.subject.meshAdulten_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshChromosomes, Human, Pair 21en_US
dc.subject.meshChromosomes, Human, Pair 8en_US
dc.subject.meshCore Binding Factor Alpha 2 Subuniten_US
dc.subject.meshDna-Binding Proteins - Geneticsen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshLeukemia, Myeloid, Acute - Genetics - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMyelodysplastic Syndromes - Genetics - Metabolismen_US
dc.subject.meshNeoplasm Proteins - Geneticsen_US
dc.subject.meshNeoplasm, Residualen_US
dc.subject.meshOncogene Proteins, Fusion - Geneticsen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshProto-Oncogene Proteinsen_US
dc.subject.meshRna, Neoplasm - Analysisen_US
dc.subject.meshSensitivity And Specificityen_US
dc.subject.meshTranscription Factors - Geneticsen_US
dc.subject.meshTranscription, Geneticen_US
dc.subject.meshTranslocation, Geneticen_US
dc.titleUse of the polymerase chain reaction in the detection of AML1/ETO fusion transcript in t(8;21)en_US
dc.typeArticleen_US
dc.identifier.emailKwong, YL:ylkwong@hku.hken_US
dc.identifier.emailChan, V:vnychana@hkucc.hku.hken_US
dc.identifier.authorityKwong, YL=rp00358en_US
dc.identifier.authorityChan, V=rp00320en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/1097-0142(19950201)75:3<821::AID-CNCR2820750312>3.0.CO;2-Zen_US
dc.identifier.pmid7828132-
dc.identifier.scopuseid_2-s2.0-0028878575en_US
dc.identifier.hkuros5615-
dc.identifier.volume75en_US
dc.identifier.issue3en_US
dc.identifier.spage821en_US
dc.identifier.epage825en_US
dc.identifier.isiWOS:A1995QC44900011-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridKwong, YL=7102818954en_US
dc.identifier.scopusauthoridChan, V=7202654865en_US
dc.identifier.scopusauthoridWong, KF=7404759860en_US
dc.identifier.scopusauthoridChan, TK=7402687762en_US

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