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Article: Localization of neutral endopeptidase (NEP) mRNA in human bronchi

TitleLocalization of neutral endopeptidase (NEP) mRNA in human bronchi
Authors
Issue Date1995
PublisherEuropean Respiratory Society. The Journal's web site is located at http://erj.ersjournals.com
Citation
European Respiratory Journal, 1995, v. 8 n. 9, p. 1458-1464 How to Cite?
AbstractNeutral endopeptidase (NEP) may regulate peptide-induced inflammation in the respiratory tract. It is of interest to determine which respiratory resident cells express NEP. Trachea and bronchi from seven nonsmoking, nonasthmatic subjects were examined. NEP messenger ribonucleic acid (mRNA) was characterized by Northern blot hybridization of cultured human tracheobronchial epithelial and smooth muscle cells, and reverse transcriptase-polymerase chain reaction (RT-PCR) in trachea and bronchi. In situ hybridization with biotin- and 35S-labelled antisense complementary ribonucleic acid (cRNA) probes was used to determine the distribution of NEP mRNA in human bronchial mucosa. NEP-immunoreactive material was detected using MEK10 murine monoclonal antibodies and the immunogold method with silver enhancement. NEP mRNA was 4.5 kb in size in the cultured human smooth muscle and epithelial cells by Northern blot analysis. No evidence was found by RT-PCR for truncated, alternatively spliced NEP mRNAs, such as del exon 16 or del exons 5-18 in human bronchus. NEP mRNA was detected by in situ hybridization in epithelial cells, submucosal glands, bronchial smooth muscle and endothelium. NEP-immunoreactive material was identified in the epithelium, submucosal glands, bronchial smooth muscle, and endothelium, demonstrating an excellent correlation between the distribution of NEP mRNA and the cell surface protein. NEP mRNA and immunoreactive material were excluded from epithelial goblet cell and submucosal gland mucous cell vacuoles. We conclude that the various sites of NEP protein and mRNA expression correlate with the locations of peptide receptors and NEP enzyme function, and are consistent with the hypothesis that NEP may regulate peptide-induced inflammation in human bronchi.
Persistent Identifierhttp://hdl.handle.net/10722/162064
ISSN
2015 Impact Factor: 8.332
2015 SCImago Journal Rankings: 3.204
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBaraniuk, JNen_US
dc.contributor.authorOhkubo, Ken_US
dc.contributor.authorKwon, OJen_US
dc.contributor.authorMak, Jen_US
dc.contributor.authorAli, Men_US
dc.contributor.authorDavies, Ren_US
dc.contributor.authorTwort, Cen_US
dc.contributor.authorKaliner, Men_US
dc.contributor.authorLetarte, Men_US
dc.contributor.authorBarnes, PJen_US
dc.date.accessioned2012-09-05T05:17:00Z-
dc.date.available2012-09-05T05:17:00Z-
dc.date.issued1995en_US
dc.identifier.citationEuropean Respiratory Journal, 1995, v. 8 n. 9, p. 1458-1464en_US
dc.identifier.issn0903-1936en_US
dc.identifier.urihttp://hdl.handle.net/10722/162064-
dc.description.abstractNeutral endopeptidase (NEP) may regulate peptide-induced inflammation in the respiratory tract. It is of interest to determine which respiratory resident cells express NEP. Trachea and bronchi from seven nonsmoking, nonasthmatic subjects were examined. NEP messenger ribonucleic acid (mRNA) was characterized by Northern blot hybridization of cultured human tracheobronchial epithelial and smooth muscle cells, and reverse transcriptase-polymerase chain reaction (RT-PCR) in trachea and bronchi. In situ hybridization with biotin- and 35S-labelled antisense complementary ribonucleic acid (cRNA) probes was used to determine the distribution of NEP mRNA in human bronchial mucosa. NEP-immunoreactive material was detected using MEK10 murine monoclonal antibodies and the immunogold method with silver enhancement. NEP mRNA was 4.5 kb in size in the cultured human smooth muscle and epithelial cells by Northern blot analysis. No evidence was found by RT-PCR for truncated, alternatively spliced NEP mRNAs, such as del exon 16 or del exons 5-18 in human bronchus. NEP mRNA was detected by in situ hybridization in epithelial cells, submucosal glands, bronchial smooth muscle and endothelium. NEP-immunoreactive material was identified in the epithelium, submucosal glands, bronchial smooth muscle, and endothelium, demonstrating an excellent correlation between the distribution of NEP mRNA and the cell surface protein. NEP mRNA and immunoreactive material were excluded from epithelial goblet cell and submucosal gland mucous cell vacuoles. We conclude that the various sites of NEP protein and mRNA expression correlate with the locations of peptide receptors and NEP enzyme function, and are consistent with the hypothesis that NEP may regulate peptide-induced inflammation in human bronchi.en_US
dc.languageengen_US
dc.publisherEuropean Respiratory Society. The Journal's web site is located at http://erj.ersjournals.comen_US
dc.relation.ispartofEuropean Respiratory Journalen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBlotting, Northernen_US
dc.subject.meshBronchi - Cytology - Enzymologyen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshEpithelium - Enzymologyen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshIn Situ Hybridizationen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMuscle, Smooth - Cytology - Enzymologyen_US
dc.subject.meshNeprilysin - Analysisen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshRna, Messenger - Analysisen_US
dc.subject.meshTrachea - Cytology - Enzymologyen_US
dc.titleLocalization of neutral endopeptidase (NEP) mRNA in human bronchien_US
dc.typeArticleen_US
dc.identifier.emailMak, J:judymak@hku.hken_US
dc.identifier.authorityMak, J=rp00352en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8575569-
dc.identifier.scopuseid_2-s2.0-0028812530en_US
dc.identifier.volume8en_US
dc.identifier.issue9en_US
dc.identifier.spage1458en_US
dc.identifier.epage1464en_US
dc.identifier.isiWOS:A1995TA29800005-
dc.publisher.placeSwitzerlanden_US
dc.identifier.scopusauthoridBaraniuk, JN=7006822080en_US
dc.identifier.scopusauthoridOhkubo, K=7101788972en_US
dc.identifier.scopusauthoridKwon, OJ=7402194121en_US
dc.identifier.scopusauthoridMak, J=7103323094en_US
dc.identifier.scopusauthoridAli, M=7404486427en_US
dc.identifier.scopusauthoridDavies, R=35372726700en_US
dc.identifier.scopusauthoridTwort, C=6701446858en_US
dc.identifier.scopusauthoridKaliner, M=35351998300en_US
dc.identifier.scopusauthoridLetarte, M=7006173454en_US
dc.identifier.scopusauthoridBarnes, PJ=36064679400en_US

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