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Article: Biphasic short-circuit current response to noradrenalin mediated by Ca 2+ and cAMP in cultured rat epididymal epithelium

TitleBiphasic short-circuit current response to noradrenalin mediated by Ca 2+ and cAMP in cultured rat epididymal epithelium
Authors
Issue Date1994
PublisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00424/index.htm
Citation
Pflugers Archiv European Journal Of Physiology, 1994, v. 426 n. 5, p. 396-401 How to Cite?
AbstractA study was carried out to investigate the short-circuit current (I(SC)) response to noradrenaline (NA) and the signal transduction mechanisms involved in cultured rat cauda epididymal epithelium. In normal Krebs-Henseleit solution, NA (10 μmol·l -1) added basolaterally elicited a biphasic I(SC) response consisting of a transient spike followed by a second sustained response. The biphasic response was almost abolished by removing ambient Cl -. Preloading the tissues with a cell permeant Ca 2+ chelator, 1,2-bis(2-aminophenoxy) ethane-N,N,N',N',-tetraacetic acid acetoxymethyl ester (BAPTA/AM), or pretreating them with thapsigargin (Tg), a microsomal adenosine triphosphatase inhibitor abolished the initial spike in the I(SC) response to NA, but had little effect on the second component. Pretreating the tissues with a non-selective β-antagonist, nadolol, reduced the second I(SC) response in a dose-dependent fashion but the initial spike was not affected. Microfluorimetric studies showed that NA (100 μmol·l -1) elicited single Ca 2+ spikes in isolated epididymal cells, which could be abolished by prior treatment with Tg. Biochemical assays showed that NA (10 μmol·l -1) increased intracellular cyclic adenosine monophosphate concentration ([cAMP](i)) and the response was abolished by prior treatment with nadolol (50 μmol·l -1). The results showed that NA elicited a biphasic I(SC) response mediated by a rise in intracellular Ca 2+ concentration ([Ca 2+](i)) followed by a rise in [cAMP](i). The Ca 2+-mediated I(SC) response had a faster onset and more transient action than the cAMP counterpart. It is suggested that NA released from noradrenergic nerve endings regulates transepithelial Cl - secretion in the epididymis thereby providing the specialized milieu vital for sperm storage and maturation.
Persistent Identifierhttp://hdl.handle.net/10722/162050
ISSN
2015 Impact Factor: 3.654
2015 SCImago Journal Rankings: 1.638
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLeung, AYHen_US
dc.contributor.authorWong, PYDen_US
dc.date.accessioned2012-09-05T05:16:54Z-
dc.date.available2012-09-05T05:16:54Z-
dc.date.issued1994en_US
dc.identifier.citationPflugers Archiv European Journal Of Physiology, 1994, v. 426 n. 5, p. 396-401en_US
dc.identifier.issn0031-6768en_US
dc.identifier.urihttp://hdl.handle.net/10722/162050-
dc.description.abstractA study was carried out to investigate the short-circuit current (I(SC)) response to noradrenaline (NA) and the signal transduction mechanisms involved in cultured rat cauda epididymal epithelium. In normal Krebs-Henseleit solution, NA (10 μmol·l -1) added basolaterally elicited a biphasic I(SC) response consisting of a transient spike followed by a second sustained response. The biphasic response was almost abolished by removing ambient Cl -. Preloading the tissues with a cell permeant Ca 2+ chelator, 1,2-bis(2-aminophenoxy) ethane-N,N,N',N',-tetraacetic acid acetoxymethyl ester (BAPTA/AM), or pretreating them with thapsigargin (Tg), a microsomal adenosine triphosphatase inhibitor abolished the initial spike in the I(SC) response to NA, but had little effect on the second component. Pretreating the tissues with a non-selective β-antagonist, nadolol, reduced the second I(SC) response in a dose-dependent fashion but the initial spike was not affected. Microfluorimetric studies showed that NA (100 μmol·l -1) elicited single Ca 2+ spikes in isolated epididymal cells, which could be abolished by prior treatment with Tg. Biochemical assays showed that NA (10 μmol·l -1) increased intracellular cyclic adenosine monophosphate concentration ([cAMP](i)) and the response was abolished by prior treatment with nadolol (50 μmol·l -1). The results showed that NA elicited a biphasic I(SC) response mediated by a rise in intracellular Ca 2+ concentration ([Ca 2+](i)) followed by a rise in [cAMP](i). The Ca 2+-mediated I(SC) response had a faster onset and more transient action than the cAMP counterpart. It is suggested that NA released from noradrenergic nerve endings regulates transepithelial Cl - secretion in the epididymis thereby providing the specialized milieu vital for sperm storage and maturation.en_US
dc.languageengen_US
dc.publisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00424/index.htmen_US
dc.relation.ispartofPflugers Archiv European Journal of Physiologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCalcium - Pharmacologyen_US
dc.subject.meshCalcium-Transporting Atpases - Antagonists & Inhibitorsen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCyclic Amp - Analysis - Pharmacologyen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshEgtazic Acid - Analogs & Derivatives - Pharmacologyen_US
dc.subject.meshEpididymis - Chemistry - Cytology - Physiologyen_US
dc.subject.meshEpithelial Cellsen_US
dc.subject.meshEpithelium - Chemistry - Physiologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMembrane Potentials - Physiologyen_US
dc.subject.meshNorepinephrine - Pharmacologyen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshSignal Transduction - Physiologyen_US
dc.subject.meshTerpenes - Pharmacologyen_US
dc.subject.meshThapsigarginen_US
dc.titleBiphasic short-circuit current response to noradrenalin mediated by Ca 2+ and cAMP in cultured rat epididymal epitheliumen_US
dc.typeArticleen_US
dc.identifier.emailLeung, AYH:ayhleung@hku.hken_US
dc.identifier.authorityLeung, AYH=rp00265en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/BF00388302-
dc.identifier.pmid8015889-
dc.identifier.scopuseid_2-s2.0-0028280798en_US
dc.identifier.volume426en_US
dc.identifier.issue5en_US
dc.identifier.spage396en_US
dc.identifier.epage401en_US
dc.identifier.isiWOS:A1994MY94900005-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridLeung, AYH=7403012668en_US
dc.identifier.scopusauthoridWong, PYD=7403980262en_US

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