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Article: Agonist-induced up-regulation of platelet-activating factor receptor messenger RNA in human monocytes

TitleAgonist-induced up-regulation of platelet-activating factor receptor messenger RNA in human monocytes
Authors
Issue Date1994
Citation
European Journal Of Pharmacology - Molecular Pharmacology Section, 1994, v. 268 n. 2, p. 263-266 How to Cite?
AbstractPlatelet-activating factor (PAF) is a potent inflammatory mediator and it actions are mediated via specific cell surface receptors which are coupled to G-proteins. PAF stimulates several functions in monocytes and may modulate the expression of its own receptor. To investigate the possible modulation of PAF receptor mRNA expression Northern blot analysis of total RNA from human monocytes was performed using the cDNA of human leukocyte PAF receptor as a probe. Following the addition of 100 nM PAF, there was a 2.0-fold increase in PAF receptor mRNA at 60 minutes after the stimulation, which was inhibited by pretreatment with the PAF receptor antagonist WEB 2086. This increase returned to control level at 120 and 180 min. The increase of PAF receptor mRNA was statistically significant for 10 nM to 1 μM of PAF, while 100 nM of lysoPAF did not increase PAF receptor mRNA levels. These results suggest that PAF receptor expression can be regulated by PAF itself at the transcriptional level.
Persistent Identifierhttp://hdl.handle.net/10722/162037
ISSN
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorShirasaki, Hen_US
dc.contributor.authorAdcock, IMen_US
dc.contributor.authorKwon, OJen_US
dc.contributor.authorNishikawa, Men_US
dc.contributor.authorMak, JCen_US
dc.contributor.authorBarnes, PJen_US
dc.date.accessioned2012-09-05T05:16:48Z-
dc.date.available2012-09-05T05:16:48Z-
dc.date.issued1994en_US
dc.identifier.citationEuropean Journal Of Pharmacology - Molecular Pharmacology Section, 1994, v. 268 n. 2, p. 263-266en_US
dc.identifier.issn0922-4106en_US
dc.identifier.urihttp://hdl.handle.net/10722/162037-
dc.description.abstractPlatelet-activating factor (PAF) is a potent inflammatory mediator and it actions are mediated via specific cell surface receptors which are coupled to G-proteins. PAF stimulates several functions in monocytes and may modulate the expression of its own receptor. To investigate the possible modulation of PAF receptor mRNA expression Northern blot analysis of total RNA from human monocytes was performed using the cDNA of human leukocyte PAF receptor as a probe. Following the addition of 100 nM PAF, there was a 2.0-fold increase in PAF receptor mRNA at 60 minutes after the stimulation, which was inhibited by pretreatment with the PAF receptor antagonist WEB 2086. This increase returned to control level at 120 and 180 min. The increase of PAF receptor mRNA was statistically significant for 10 nM to 1 μM of PAF, while 100 nM of lysoPAF did not increase PAF receptor mRNA levels. These results suggest that PAF receptor expression can be regulated by PAF itself at the transcriptional level.en_US
dc.languageengen_US
dc.relation.ispartofEuropean Journal of Pharmacology - Molecular Pharmacology Sectionen_US
dc.subject.meshAdulten_US
dc.subject.meshAzepines - Pharmacologyen_US
dc.subject.meshGene Expression Regulation - Drug Effectsen_US
dc.subject.meshHumansen_US
dc.subject.meshMonocytes - Metabolismen_US
dc.subject.meshPlatelet Activating Factor - Pharmacologyen_US
dc.subject.meshPlatelet Membrane Glycoproteins - Agonists - Geneticsen_US
dc.subject.meshRna, Messenger - Analysisen_US
dc.subject.meshReceptors, Cell Surfaceen_US
dc.subject.meshReceptors, G-Protein-Coupleden_US
dc.subject.meshTriazoles - Pharmacologyen_US
dc.subject.meshUp-Regulationen_US
dc.titleAgonist-induced up-regulation of platelet-activating factor receptor messenger RNA in human monocytesen_US
dc.typeArticleen_US
dc.identifier.emailMak, JC:judymak@hku.hken_US
dc.identifier.authorityMak, JC=rp00352en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0922-4106(94)90197-Xen_US
dc.identifier.pmid7957648-
dc.identifier.scopuseid_2-s2.0-0028169087en_US
dc.identifier.volume268en_US
dc.identifier.issue2en_US
dc.identifier.spage263en_US
dc.identifier.epage266en_US
dc.identifier.isiWOS:A1994NY37800018-
dc.identifier.scopusauthoridShirasaki, H=7004586584en_US
dc.identifier.scopusauthoridAdcock, IM=7007066538en_US
dc.identifier.scopusauthoridKwon, OJ=7402194121en_US
dc.identifier.scopusauthoridNishikawa, M=7402607361en_US
dc.identifier.scopusauthoridMak, JC=7103323094en_US
dc.identifier.scopusauthoridBarnes, PJ=36064679400en_US

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