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Article: Long-term exposure to norepinephrine results in down-regulation and reduced mRNA expression of pulmonary beta-adrenergic receptors in guinea pigs.

TitleLong-term exposure to norepinephrine results in down-regulation and reduced mRNA expression of pulmonary beta-adrenergic receptors in guinea pigs.
Authors
Issue Date1994
PublisherAmerican Thoracic Society. The Journal's web site is located at http://ajrcmb.atsjournals.org
Citation
American Journal Of Respiratory Cell And Molecular Biology, 1994, v. 10 n. 1, p. 91-99 How to Cite?
AbstractWe studied the down-regulation of beta 2-adrenoceptors in guinea pig lung after chronic exposure to a beta-agonist. Guinea pigs were exposed to either norepinephrine (NE) or vehicle for 7 days. The density and affinity of beta-adrenoceptors were determined by Scatchard analysis of [125I]cyanopindolol binding in a lung membrane preparation and beta 1- and beta 2-adrenoceptor subtypes were studied in the presence of 0.1 microM ICI 118,551, a selective beta 2-adrenoceptor antagonist, or 0.1 microM CGP 20712 A, a selective beta 1-adrenoceptor antagonist, respectively. beta 2-Adrenoceptor mRNA was examined by Northern blot analysis. The tissue distribution of beta 2-adrenoceptors and of beta 2-adrenoceptor mRNA in lung after NE infusion were determined using receptor autoradiography and in situ hybridization, respectively. The functional significance of the decrease in beta 2-adrenoceptor was tested by measuring the relaxation response to a beta 2-agonist in isolated parenchymal strips. NE treatment resulted in decreases of 75 +/- 9%, 84 +/- 4%, and 66 +/- 9% of total beta-, beta 1-, and beta 2-adrenoceptors in the lung, compared with control animals. The administration of NE had only minimal effects on the dissociation constant (Kd) of the receptor for the radioligand. beta 2-Adrenoceptors mRNA was decreased by 46 +/- 13% after NE treatment. There was a correspondence between the distribution of beta 2-adrenoceptors and beta 2-adrenoceptor mRNA localization in both control and NE-treated guinea pigs. NE treatment reduced alveolar beta 2-adrenoceptors by 70 +/- 3% and its mRNA expression by 78 +/- 5%.(ABSTRACT TRUNCATED AT 250 WORDS)
Persistent Identifierhttp://hdl.handle.net/10722/162036
ISSN
2015 Impact Factor: 4.082
2015 SCImago Journal Rankings: 1.622
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorNishikawa, Men_US
dc.contributor.authorMak, JCen_US
dc.contributor.authorShirasaki, Hen_US
dc.contributor.authorHarding, SEen_US
dc.contributor.authorBarnes, PJen_US
dc.date.accessioned2012-09-05T05:16:48Z-
dc.date.available2012-09-05T05:16:48Z-
dc.date.issued1994en_US
dc.identifier.citationAmerican Journal Of Respiratory Cell And Molecular Biology, 1994, v. 10 n. 1, p. 91-99en_US
dc.identifier.issn1044-1549en_US
dc.identifier.urihttp://hdl.handle.net/10722/162036-
dc.description.abstractWe studied the down-regulation of beta 2-adrenoceptors in guinea pig lung after chronic exposure to a beta-agonist. Guinea pigs were exposed to either norepinephrine (NE) or vehicle for 7 days. The density and affinity of beta-adrenoceptors were determined by Scatchard analysis of [125I]cyanopindolol binding in a lung membrane preparation and beta 1- and beta 2-adrenoceptor subtypes were studied in the presence of 0.1 microM ICI 118,551, a selective beta 2-adrenoceptor antagonist, or 0.1 microM CGP 20712 A, a selective beta 1-adrenoceptor antagonist, respectively. beta 2-Adrenoceptor mRNA was examined by Northern blot analysis. The tissue distribution of beta 2-adrenoceptors and of beta 2-adrenoceptor mRNA in lung after NE infusion were determined using receptor autoradiography and in situ hybridization, respectively. The functional significance of the decrease in beta 2-adrenoceptor was tested by measuring the relaxation response to a beta 2-agonist in isolated parenchymal strips. NE treatment resulted in decreases of 75 +/- 9%, 84 +/- 4%, and 66 +/- 9% of total beta-, beta 1-, and beta 2-adrenoceptors in the lung, compared with control animals. The administration of NE had only minimal effects on the dissociation constant (Kd) of the receptor for the radioligand. beta 2-Adrenoceptors mRNA was decreased by 46 +/- 13% after NE treatment. There was a correspondence between the distribution of beta 2-adrenoceptors and beta 2-adrenoceptor mRNA localization in both control and NE-treated guinea pigs. NE treatment reduced alveolar beta 2-adrenoceptors by 70 +/- 3% and its mRNA expression by 78 +/- 5%.(ABSTRACT TRUNCATED AT 250 WORDS)en_US
dc.languageengen_US
dc.publisherAmerican Thoracic Society. The Journal's web site is located at http://ajrcmb.atsjournals.orgen_US
dc.relation.ispartofAmerican journal of respiratory cell and molecular biologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBlotting, Northernen_US
dc.subject.meshDown-Regulationen_US
dc.subject.meshGuinea Pigsen_US
dc.subject.meshIn Situ Hybridizationen_US
dc.subject.meshLung - Drug Effects - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshNorepinephrine - Pharmacologyen_US
dc.subject.meshRna, Messenger - Drug Effects - Metabolismen_US
dc.subject.meshReceptors, Adrenergic, Beta-1 - Drug Effects - Metabolismen_US
dc.subject.meshReceptors, Adrenergic, Beta-2 - Drug Effects - Metabolismen_US
dc.subject.meshTime Factorsen_US
dc.titleLong-term exposure to norepinephrine results in down-regulation and reduced mRNA expression of pulmonary beta-adrenergic receptors in guinea pigs.en_US
dc.typeArticleen_US
dc.identifier.emailMak, JC:judymak@hku.hken_US
dc.identifier.authorityMak, JC=rp00352en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1165/ajrcmb.10.1.8292387-
dc.identifier.pmid8292387en_US
dc.identifier.scopuseid_2-s2.0-0028135973en_US
dc.identifier.volume10en_US
dc.identifier.issue1en_US
dc.identifier.spage91en_US
dc.identifier.epage99en_US
dc.identifier.isiWOS:A1994MT98200013-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridNishikawa, M=7402607361en_US
dc.identifier.scopusauthoridMak, JC=7103323094en_US
dc.identifier.scopusauthoridShirasaki, H=7004586584en_US
dc.identifier.scopusauthoridHarding, SE=7202447604en_US
dc.identifier.scopusauthoridBarnes, PJ=36064679400en_US

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