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Article: Modulation of airway smooth muscle β-adrenoceptor function by a muscarinic agonist

TitleModulation of airway smooth muscle β-adrenoceptor function by a muscarinic agonist
Authors
Issue Date1994
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie
Citation
Life Sciences, 1994, v. 54 n. 3, p. 185-191 How to Cite?
AbstractWe have investigated the effect of a muscarinic agonist and protein kinase C (PKC) activation on β-adrenoceptors and their coupling to adenylyl cyclase in bovine tracheal smooth muscle. There was a significant reduction in maximum binding capacity of [125I]iodocyanopindolol ([125I]ICYP) after exposure to carbachol and 4β-phorbol 12β-myristate 13α-acetate (PMA). Similarly both carbachol and PMA inhibited the 5'-guanylylimidodiphosphate-induced shift in [125I]ICYP binding by isoproterenol and significantly decreased isoproterenol-induced cyclic AMP accumulation. A phorbol ester, 4α-phorbol 12,13-didecanoate which does not activate PKC had no effect on β-receptor binding or coupling. These results suggest that PKC activation directly via a phorbol ester and indirectly via muscarinic receptor stimulation may lead to reduction and uncoupling of β-receptors in airway smooth muscle. We suggest that this mechanism may be relevant to the reduction in β-receptor coupling in asthmatic airways as an effect of PKC activation by inflammatory mediators and neurotransmitters.
Persistent Identifierhttp://hdl.handle.net/10722/162027
ISSN
2015 Impact Factor: 2.685
2015 SCImago Journal Rankings: 1.056
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorGrandordy, BMen_US
dc.contributor.authorMak, JCWen_US
dc.contributor.authorBarnes, PJen_US
dc.date.accessioned2012-09-05T05:16:45Z-
dc.date.available2012-09-05T05:16:45Z-
dc.date.issued1994en_US
dc.identifier.citationLife Sciences, 1994, v. 54 n. 3, p. 185-191en_US
dc.identifier.issn0024-3205en_US
dc.identifier.urihttp://hdl.handle.net/10722/162027-
dc.description.abstractWe have investigated the effect of a muscarinic agonist and protein kinase C (PKC) activation on β-adrenoceptors and their coupling to adenylyl cyclase in bovine tracheal smooth muscle. There was a significant reduction in maximum binding capacity of [125I]iodocyanopindolol ([125I]ICYP) after exposure to carbachol and 4β-phorbol 12β-myristate 13α-acetate (PMA). Similarly both carbachol and PMA inhibited the 5'-guanylylimidodiphosphate-induced shift in [125I]ICYP binding by isoproterenol and significantly decreased isoproterenol-induced cyclic AMP accumulation. A phorbol ester, 4α-phorbol 12,13-didecanoate which does not activate PKC had no effect on β-receptor binding or coupling. These results suggest that PKC activation directly via a phorbol ester and indirectly via muscarinic receptor stimulation may lead to reduction and uncoupling of β-receptors in airway smooth muscle. We suggest that this mechanism may be relevant to the reduction in β-receptor coupling in asthmatic airways as an effect of PKC activation by inflammatory mediators and neurotransmitters.en_US
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescieen_US
dc.relation.ispartofLife Sciencesen_US
dc.subject.meshAdrenergic Beta-Agonists - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBinding, Competitiveen_US
dc.subject.meshCarbachol - Pharmacologyen_US
dc.subject.meshCattleen_US
dc.subject.meshCyclic Amp - Metabolismen_US
dc.subject.meshEnzyme Activationen_US
dc.subject.meshGtp-Binding Proteins - Metabolismen_US
dc.subject.meshIsoproterenol - Metabolism - Pharmacologyen_US
dc.subject.meshKineticsen_US
dc.subject.meshMuscle, Smooth - Drug Effects - Metabolism - Ultrastructureen_US
dc.subject.meshProtein Kinase C - Metabolismen_US
dc.subject.meshRadioligand Assayen_US
dc.subject.meshReceptors, Adrenergic, Beta - Drug Effects - Metabolismen_US
dc.subject.meshTetradecanoylphorbol Acetate - Pharmacologyen_US
dc.subject.meshTrachea - Drug Effects - Metabolism - Ultrastructureen_US
dc.titleModulation of airway smooth muscle β-adrenoceptor function by a muscarinic agonisten_US
dc.typeArticleen_US
dc.identifier.emailMak, JCW:judymak@hku.hken_US
dc.identifier.authorityMak, JCW=rp00352en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0024-3205(94)00587-7en_US
dc.identifier.pmid7904709en_US
dc.identifier.scopuseid_2-s2.0-0028009707en_US
dc.identifier.volume54en_US
dc.identifier.issue3en_US
dc.identifier.spage185en_US
dc.identifier.epage191en_US
dc.identifier.isiWOS:A1994MM38300005-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridGrandordy, BM=6701645822en_US
dc.identifier.scopusauthoridMak, JCW=7103323094en_US
dc.identifier.scopusauthoridBarnes, PJ=36064679400en_US

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