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Article: Identification of neutral endopeptidase mRNA in human nasal mucosa

TitleIdentification of neutral endopeptidase mRNA in human nasal mucosa
Authors
Issue Date1993
Citation
Journal Of Applied Physiology, 1993, v. 74 n. 1, p. 272-279 How to Cite?
AbstractNeutral endopeptidase (NEP) may regulate peptide-induced inflammation in the upper respiratory tract. NEP mRNA was detected by Northern blotting in poly(A)+ mRNA prepared from human turbinates. NEP mRNA bands were detected at 3.9 and 1.8 kb. In situ hybridization identified NEP mRNA in epithelial cells, serous cells of submucosal glands, and vessel walls. The vacuoles of goblet and mucous cells were not stained, but it is likely that the cytoplasm of these cells contained some NEP mRNA. Radioactive in situ hybridization with 35S-UTP appeared to be more sensitive than nonradioactive in situ hybridization with biotin-UTP and immunogold detection. The NEP mRNA- containing cells identified by in situ hybridization were the same as those identified by immunohistochemistry to contain immunoreactive NEP. These NEP- containing cells have been previously shown to possess peptide receptors. This is consistent with the hypothesis that NEP on cells bearing peptide receptors may regulate neuropeptide-induced inflammation in human nasal mucosa.
Persistent Identifierhttp://hdl.handle.net/10722/162013
ISSN
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBaraniuk, JNen_US
dc.contributor.authorOhkubo, Ken_US
dc.contributor.authorKwon, OJen_US
dc.contributor.authorMak, Jen_US
dc.contributor.authorRohde, Jen_US
dc.contributor.authorKaliner, MAen_US
dc.contributor.authorDurham, SRen_US
dc.contributor.authorBarnes, PJen_US
dc.date.accessioned2012-09-05T05:16:41Z-
dc.date.available2012-09-05T05:16:41Z-
dc.date.issued1993en_US
dc.identifier.citationJournal Of Applied Physiology, 1993, v. 74 n. 1, p. 272-279en_US
dc.identifier.issn0161-7567en_US
dc.identifier.urihttp://hdl.handle.net/10722/162013-
dc.description.abstractNeutral endopeptidase (NEP) may regulate peptide-induced inflammation in the upper respiratory tract. NEP mRNA was detected by Northern blotting in poly(A)+ mRNA prepared from human turbinates. NEP mRNA bands were detected at 3.9 and 1.8 kb. In situ hybridization identified NEP mRNA in epithelial cells, serous cells of submucosal glands, and vessel walls. The vacuoles of goblet and mucous cells were not stained, but it is likely that the cytoplasm of these cells contained some NEP mRNA. Radioactive in situ hybridization with 35S-UTP appeared to be more sensitive than nonradioactive in situ hybridization with biotin-UTP and immunogold detection. The NEP mRNA- containing cells identified by in situ hybridization were the same as those identified by immunohistochemistry to contain immunoreactive NEP. These NEP- containing cells have been previously shown to possess peptide receptors. This is consistent with the hypothesis that NEP on cells bearing peptide receptors may regulate neuropeptide-induced inflammation in human nasal mucosa.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Applied Physiologyen_US
dc.subject.meshBiotinen_US
dc.subject.meshBlotting, Northernen_US
dc.subject.meshEndopeptidases - Biosynthesisen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshIn Situ Hybridizationen_US
dc.subject.meshNasal Mucosa - Chemistry - Metabolismen_US
dc.subject.meshParaffin Embeddingen_US
dc.subject.meshPlasmidsen_US
dc.subject.meshProtein Biosynthesisen_US
dc.subject.meshRna Probesen_US
dc.subject.meshRna, Antisense - Metabolismen_US
dc.subject.meshRna, Messenger - Analysis - Metabolismen_US
dc.subject.meshTranscription, Geneticen_US
dc.titleIdentification of neutral endopeptidase mRNA in human nasal mucosaen_US
dc.typeArticleen_US
dc.identifier.emailMak, J:judymak@hku.hken_US
dc.identifier.authorityMak, J=rp00352en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8444703-
dc.identifier.scopuseid_2-s2.0-0027536940en_US
dc.identifier.volume74en_US
dc.identifier.issue1en_US
dc.identifier.spage272en_US
dc.identifier.epage279en_US
dc.identifier.isiWOS:A1993KJ48000037-
dc.identifier.scopusauthoridBaraniuk, JN=7006822080en_US
dc.identifier.scopusauthoridOhkubo, K=7101788972en_US
dc.identifier.scopusauthoridKwon, OJ=7402194121en_US
dc.identifier.scopusauthoridMak, J=7103323094en_US
dc.identifier.scopusauthoridRohde, J=7102243322en_US
dc.identifier.scopusauthoridKaliner, MA=35351998300en_US
dc.identifier.scopusauthoridDurham, SR=35351287700en_US
dc.identifier.scopusauthoridBarnes, PJ=36064679400en_US

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